1,25-Dihydroxyvitamin D increases the gene expression of enzymes protecting from glucolipotoxicity in peripheral blood mononuclear cells and human primary endothelial cells

2016 ◽  
Vol 7 (6) ◽  
pp. 2537-2543 ◽  
Author(s):  
Katarina Kuricova ◽  
Anna Pleskacova ◽  
Lukas Pacal ◽  
Katerina Kankova
Keyword(s):  
Gene Expression ◽  
Vitamin D ◽  
Endothelial Cells ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Dihydroxyvitamin D ◽  
1,25 Dihydroxyvitamin D ◽  
Blood Mononuclear Cells ◽  
Classical Function ◽  
Calcium And Phosphorus

Besides its classical function as an orchestrator of calcium and phosphorus homeostasis, vitamin D also affects insulin secretion and tissue efficiency.

scholarly journals Plasma 25-Hydroxyvitamin D Levels and VDR Gene Expression in Peripheral Blood Mononuclear Cells of Leukemia Patients and Healthy Subjects in Central Kazakhstan

Nutrients ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1229
Author(s):  
Assel G. Zhumina ◽  
Konstantin Li ◽  
Anna A. Konovalova ◽  
Yelena A. Li ◽  
Margarita Yu. Ishmuratova ◽  
...  
Keyword(s):  
Gene Expression ◽  
Vitamin D ◽  
Healthy Subjects ◽  
Mononuclear Cells ◽  
Vdr Gene ◽  
Peripheral Blood Mononuclear ◽  
Hydroxyvitamin D ◽  
Blood Mononuclear Cells ◽  
25 Hydroxyvitamin D ◽  
Gene Expression Levels

Low blood levels of the vitamin D metabolite 25-hydroxyvitamin D [25(OH)D] have been associated with an increased risk and poorer outcomes of various cancers, including hematological malignancies. The Central Kazakhstan area has a relatively high incidence rate of leukemia. However, the relationship between vitamin D status and leukemia or other types of cancer in Kazakhstan has not yet been addressed. Therefore, in this first pilot single-center study conducted in Central Kazakhstan, we compared plasma levels of 25(OH)D and the vitamin D receptor (VDR) gene expression levels in peripheral blood mononuclear cells of patients with leukemia and demographically matching healthy volunteers. The levels of 25(OH)D in patients were found to be significantly lower (10.8 ± 7.0 ng/mL; n = 31) than in healthy subjects (21.6 ± 7.8 ng/mL; n = 34; p < 0.0001). A similar difference was observed in both younger (<60 years old) and older (>60 years old) participants, though there was no association between 25(OH)D concentration and age within the patient group. In female patients, 25(OH)D levels were significantly lower than in male patients (p = 0.04). No significant seasonal variations of 25(OH)D were observed in either the patient or the control group. VDR gene expression levels appeared to be similar in leukemia patients and healthy subjects, and no correlation between the cellular VDR expression and plasma 25(OH)D concentrations was observed in either group of participants. We did not observe a significant association of 25(OH)D or VDR levels and overall survival of leukemia patients. This observational study conducted for the first time in Kazakhstan supports previous findings demonstrating reduced blood 25(OH)D levels in cancer (leukemia) patients. Larger studies are required to determine whether low 25(OH)D plasma concentrations represent a risk factor for leukemia development and/or progression.

Gene Expression Profiling of Human Endothelial Progenitor Cells Differentiated from Peripheral Blood Mononuclear Cells.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 3911-3911
Author(s):  
Beatrice E. Gee ◽  
Jerry J. Manlove-Simmons ◽  
Yao Huang ◽  
Kwaku Asare ◽  
Adel Driss ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Endothelial Cell ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Expression Profiling ◽  
Mononuclear Cells ◽  
Differentially Expressed ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Abstract Endothelial progenitor cells (EPCs) originate in bone marrow and participate in revascularization and angiogenesis. EPCs can be differentiated from bone marrow, umbilical cord, or peripheral blood cells. In order to better understand the genetic regulation of EPC differentiation, we have characterized genes that are differentially expressed by human EPCs after their development from peripheral blood mononuclear cells, and compared them to genes expressed by endothelial cells from arteries, veins, and microvessels. Methods: Peripheral blood mononuclear cells (MNC) were isolated from venous blood of healthy human volunteers by density fractionation. MNC were differentiated into EPCs by culturing on fibronectin-coated plates with EMG2-MV (microvascular endothelial) media (Lonza). Total RNA was collected from freshly isolated MNC and EPCs after 7 days of culture. Gene expression profiling was performed using 44k 60-mer Human Whole Genome oligo array (Agilent). Data were analyzed using GeneSpring and Ingenuity Pathways Analysis Enterprise Edition. MNC- and EPC-expressed genes were compared to gene expression profiles of 53 samples of human artery, vein, and microvessels obtained from the Stanford Microarray database. Data were filtered to identify genes that were more than 2-fold differentially expressed between EPCs or endothelial cells when compared to MNCs. Multiple testing correction was applied to achieve statistical significance p&lt;0.05. Ingenuity Pathways Analysis was used to identify functional categories and canonical networks. Gene expression was validated by quantitative RT-PCR. Results: Six samples of MNC and 4 samples of EPCs were analyzed by microarray. A total of 356 genes were differentially expressed in EPCs in comparison to MNCs (&gt;2-fold, p&lt;0.05). The highest expressed was Glycoprotein nmb (osteoactivin, +1718-fold) and the lowest was the platelet factor 4 (−100-fold). Combined results of EPCs, MNCs, and Stanford endothelial cell samples revealed 6,051 commonly expressed genes. We found 236 genes that were at least 2-fold differentially expressed in EPCs and all endothelial samples when compared to MNC. These genes fall into the following categories: cell growth and proliferation (68 genes), cell-cell signaling and interaction (60), cell death (55), cell signaling (68), hematologic system development and function (50), cardiovascular function (18). As expected, expression of genes with hematopoietic or immune functions, such as platelet factor 4, nuclear factor erythroid-derived (NFE) 2, CD8, and T-cell receptor, were lower in EPCs or endothelial cells versus MNC. EPCs and endothelial cells had higher expression of a number of genes related to cell-cell interaction, including chemokine ligand 18, selenoprotein P (SEPP1), and matrix metalloproteinases (MMP) 7 and 14. Hierarchical clustering of EPCs and all endothelial cell samples showed EPCs to be most similar to skin microvessels (correlation coefficient 0.848). RT-PCR results confirmed differential expression in EPCs versus MNCs in 8 out of 9 genes tested. Conclusions: We have used gene expression profiling to identify genes that may be important in endothelial cell lineage commitment during EPC differentiation from peripheral blood mononuclear cells. These findings may provide valuable insight for the future development of treatment modalities for vessel repair and homeostasis.

scholarly journals The Effect of Sulforaphane on Glyoxalase I Expression and Activity in Peripheral Blood Mononuclear Cells

Nutrients ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1773 ◽  
Author(s):  
Michela Alfarano ◽  
Donato Pastore ◽  
Vincenzo Fogliano ◽  
Casper Schalkwijk ◽  
Teresa Oliviero
Keyword(s):  
Gene Expression ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Limit Of Detection ◽  
Peripheral Blood Mononuclear ◽  
Potential Health ◽  
Age Related ◽  
Blood Mononuclear Cells ◽  
Expression Activity

Studies demonstrate that the potential health-beneficial effect of sulforaphane (SR), a compound formed in broccoli, is the result of a number of mechanisms including upregulation of phase two detoxification enzymes. Recent studies suggest that SR increases expression/activity of glyoxalase 1 (Glo1), an enzyme involved in the degradation of methylglyoxal, is major precursor of advanced glycation end products. Those compounds are associated with diabetes complications and other age-related diseases. In this study, the effect of SR on the expression/activity of Glo1 in peripheral blood mononuclear cells (PBMCs) from 8 healthy volunteers was investigated. PBMCs were isolated and incubated with SR (2.5 μM-concentration achievable by consuming a broccoli portion) for 24 h and 48 h. Glo1 activity/expression, reduced glutathione (GSH), and glutathione-S-transferase gene expression were measured. Glo1 activity was not affected while after 48 h a slight but significant increase of its gene expression (1.03-fold) was observed. GSTP1 expression slightly increased after 24 h incubation (1.08-fold) while the expressions of isoform GSTT2 and GSTM2 were below the limit of detection. GSH sharply decreased, suggesting the formation of GSH-SR adducts that may have an impact SR availability. Those results suggest that a regular exposure to SR by broccoli consumption or SR supplements may enhance Glo1.

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