A protective effect of anthocyanins and xanthophylls on UVB-induced damage in retinal pigment epithelial cells

2016 ◽  
Vol 7 (2) ◽  
pp. 1067-1076 ◽  
Author(s):  
Jose Manuel Silván ◽  
Marina Reguero ◽  
Sonia de Pascual-Teresa
Keyword(s):  
Epithelial Cells ◽  
Protective Effect ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Retinal Pigment Epithelial Cells ◽  
Mapk Activation ◽  
Retinal Cells ◽  
Pigment Epithelial ◽  
Pigment Epithelial Cells

Cyanidin-3-glucoside and zeaxanthin, alone or in combination, protect against UVB-induced retinal cells damage throw redox and MAPK activation regulation.

scholarly journals Memantine, Simvastatin, and Epicatechin Inhibit 7-Ketocholesterol-induced Apoptosis in Retinal Pigment Epithelial Cells But Not Neurosensory Retinal Cells In Vitro

2020 ◽  
Author(s):  
Aneesh Neekhra ◽  
Julia Tran ◽  
Khoa Pham ◽  
Ashish Sharma ◽  
Marilyn Chwa ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Retinal Pigment Epithelial ◽  
Caspase 3 ◽  
Retinal Pigment ◽  
Retinal Pigment Epithelial Cells ◽  
Retinal Cells ◽  
Pigment Epithelial ◽  
Change In Mean ◽  
Pigment Epithelial Cells

Purpose: 7-ketocholesterol (7kCh), a natural byproduct of oxidation in lipoprotein deposits, is implicated in the pathogenesis of diabetic retinopathy and age-related macular degeneration (AMD). This study was performed to investigate whether several clinical drugs can inhibit 7kCh-induced caspase activation and mitigate its apoptotic effects on retinal cells in vitro. Method: Two populations of retinal cells, human retinal pigment epithelial cells (ARPE-19) and rat neuroretinal cells (R28), were exposed to 7kCh in the presence of the following inhibitors: Z-VAD-FMK (pan-caspase inhibitor), simvastatin, memantine, epicatechin, and Z-IETD-FMK (caspase-8 inhibitor) or Z-ATAD-FMK (caspase-12 inhibitor). Caspase-3/7, -8, and -12 activity levels were measured by fluorochrome caspase assays to quantify cell death. IncuCyte live-cell microscopic images were obtained to quantify cell counts. Results: Exposure to 7kCh for 24 hours significantly increased caspase activities for both ARPE-19 and R28 cells (P < 0.05). In ARPE cells, pretreatment with various drugs had significantly lower caspase-3/7, -8, and -12 activities, reported in % change in mean signal intensity (msi): Z-VAD-FMK (48% decrease, P < 0.01), memantine (decreased 47.8% at 1 μM, P = 0.0039 and 81.9% at 1 mM, P < 0.001), simvastatin (decreased 85.3% at 0.01 μM, P < 0.001 and 84.8% at 0.05 μM , P < 0.001) or epicatechin (83.6% decrease, P < 0.05), Z-IETD-FMK (68.1% decrease, P < 0.01), and Z-ATAD-FMK (47.7% decrease, P = 0.0017). In contrast, R28 cells exposed to 7kCh continued to have elevated caspase- 3/7, -8, and -12 activities (between 25.7% decrease and 17.5% increase in msi, P > 0.05) regardless of the pretreatment. Conclusion: Several current drugs protect ARPE-19 cells but not R28 cells from 7kChinduced apoptosis, suggesting that a multiple-drug approach is needed to protect both cells types in various retinal diseases.

scholarly journals Retino-protective effect of Bucida buceras against oxidative stress induced by H2O2 in human retinal pigment epithelial cells line

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Simon Bernard Iloki-Assanga ◽  
Lidianys María Lewis-Luján ◽  
Daniela Fernández-Angulo ◽  
Armida Andrea Gil-Salido ◽  
Claudia Lizeth Lara-Espinoza ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Epithelial Cells ◽  
Protective Effect ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Retinal Pigment Epithelial Cells ◽  
Pigment Epithelial ◽  
Pigment Epithelial Cells
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