A novel multi-biofunctional protein from brown rice hydrolysed by endo/endo-exoproteases

2016 ◽  
Vol 7 (6) ◽  
pp. 2635-2644 ◽  
Author(s):  
Orrapun Selamassakul ◽  
Natta Laohakunjit ◽  
Orapin Kerdchoechuen ◽  
Khanok Ratanakhanokchai
Keyword(s):  
Antioxidant Activities ◽  
Protein Hydrolysate ◽  
Brown Rice ◽  
P Protein

Protein hydrolysate from brown rice is a new source of great multifunctional peptide (biological antioxidant activities, biofunctional properties and bioflavor).

scholarly journals Anti-Atherogenic Effect of 10% Supplementation of Anchovy (Engraulis encrasicolus) Waste Protein Hydrolysates in ApoE-Deficient Mice

Nutrients ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 2137
Author(s):  
Jessica Maria Abbate ◽  
Francesco Macrì ◽  
Francesca Arfuso ◽  
Carmelo Iaria ◽  
Fabiano Capparucci ◽  
...  
Keyword(s):  
Human Health ◽  
Antioxidant Activities ◽  
Protein Hydrolysate ◽  
Protein Hydrolysates ◽  
Metabolic Effects ◽  
Lipid Lowering ◽  
High Frequency Ultrasound ◽  
Aortic Sinus ◽  
Fish Waste ◽  
Plaque Development

Fish protein consumption exerts beneficial metabolic effects on human health, also correlating with a decreased risk for cardiovascular disease. Fish waste contains high amount of proteins and utilization may offer the opportunity for generating compounds advantageous for human health. Especially, fish waste protein hydrolysates beneficially influence pathways involved in body composition, exerting anti-inflammatory and antioxidant activities, making their potential supplementation in human disorders of increased interest. This study assessed the effect of a 10% (w/w) anchovy waste protein hydrolysate (APH) diet for 12 weeks in reducing atherosclerosis in ApoE−/− mice, through histological and immunohistochemical methods. In addition, monitoring of plaque development was performed, using high-frequency ultrasound and magnetic resonance imaging. Overall, the APH diet attenuated atherosclerotic plaque development, producing a regression of arterial lesions over time (p < 0.05). Twelve weeks on an APH diet had an anti-obesity effect, improving lipid metabolism and reducing hepatic enzyme activity. A significant reduction in plaque size and lipid content was observed in the aortic sinus of APH-fed mice, compared to the control (p < 0.001), whereas no differences in the extracellular matrix and macrophage recruitment were observed. Supplementation of APH significantly attenuates atherosclerosis in ApoE−/− mice, exerting a lipid-lowering activity. The opportunity to use fish waste protein hydrolysates as a nutraceutical in atherosclerosis is worthy of future investigations, representing a low cost, sustainable, and nutritional strategy with minimal environmental impact.

scholarly journals Chemical and Cellular Antioxidant Activities of In Vitro Digesta of Tilapia Protein and Its Hydrolysates

Foods ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 833
Author(s):  
Xiaogang Zhang ◽  
Parinya Noisa ◽  
Jirawat Yongsawatdigul
Keyword(s):  
Structural Modification ◽  
Physiological Condition ◽  
Antioxidant Activities ◽  
Protein Hydrolysate ◽  
Principal Component ◽  
Hydrogen Atom Transfer ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Degree Of Hydrolysis ◽  
Trolox Equivalent

Production of protein hydrolysate as nutraceuticals is typically based on the activity of the hydrolysate, which might not yield the optimal activity under physiological condition due to structural modification of peptides upon gastrointestinal (GI) digestion. This study systematically compared the chemical and cellular antioxidant activities of the in vitro digesta of tilapia protein and its hydrolysates prepared with various degree of hydrolysis (DH) by Alcalase. The enzymes used in the in vitro GI digestion analysis significantly contributed to the peptide content, Trolox equivalent antioxidant capacity (TEAC), and oxygen radical absorbance capacity (ORAC). Proteins and all hydrolysates were slightly digested by pepsin but hydrolyzed extensively by pancreatin. Both hydrolysate and digesta predominantly scavenged free radicals via hydrogen atom transfer (HAT). The antioxidant activities of the hydrolysates increased with the increasing DH up to 16 h of hydrolysis. However, the digesta of 10-h hydrolysate displayed the highest chemical and HepG2 cellular antioxidant activities, while the protein digesta displayed the lowest. Principal component analysis (PCA) showed that the TEAC of the digesta was positively correlated with the cellular antioxidant activity (CAA). Therefore, the production of protein hydrolysate should be optimized based on the activity of the hydrolysate digesta rather than that of hydrolysates.

scholarly journals Foliar Applications of Protein Hydrolysate, Plant and Seaweed Extracts Increase Yield but Differentially Modulate Fruit Quality of Greenhouse Tomato

HortScience ◽  
2017 ◽  
Vol 52 (9) ◽  
pp. 1214-1220 ◽  
Author(s):  
Giuseppe Colla ◽  
Mariateresa Cardarelli ◽  
Paolo Bonini ◽  
Youssef Rouphael
Keyword(s):  
Antioxidant Activities ◽  
Protein Hydrolysate ◽  
Total Yield ◽  
Economic Benefits ◽  
Soluble Solids ◽  
Total Production ◽  
Marketable Yield ◽  
Soil Culture ◽  
Yield Performance ◽  
Greenhouse Tomato

The current research aimed 1) at evaluating the effects of three biostimulants (legume-derived protein hydrolysate, PH; plant and seaweed extract, PE and SWE) on yield performance and nutritional quality, mineral profiling, antioxidant activities, lycopene, total phenols and ascorbic acid of greenhouse tomato (Solanum lycopersicum L.) under soil culture and 2) to assess the economic profitability of biostimulant applications. Plants were sprayed four times during the growing cycle with a solution containing 1, 3, and 3 mL·L−1 of PE, SWE, and PH, respectively. Foliar applications of biostimulants improved the early and total marketable yield of fresh tomato. The increase of total yield by PE, SWE, and PH was 11.7%, 6.6% and 7.0%, respectively, in comparison with untreated plants. Legume-derived PH increased lycopene, total soluble solids, and K and Mg contents, thereby increasing the nutritional value of the fruits. The applications of SWE, and to a lesser degree PH, enhanced the Ca concentration in the fruit tissue. Our findings indicated that the three tested biostimulants, although they increased the total production cost, improved the nutrient status and yield performance of the crop to a level resulting in net economic benefits.

Purification, identification, and in vitro antioxidant activities of selenium-containing proteins from selenium-enriched brown rice

2011 ◽  
Vol 234 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Kunlun Liu ◽  
Yan Zhao ◽  
Fusheng Chen ◽  
Zhenxin Gu ◽  
Guanhao Bu
Keyword(s):  
Antioxidant Activities ◽  
Brown Rice

scholarly journals Angiotensin-I-Converting Enzyme Inhibitory and Antioxidant Activities of Protein Hydrolysate from Muscle of Barbel (Barbus callensis)

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Assaad Sila ◽  
Anissa Haddar ◽  
Oscar Martinez-Alvarez ◽  
Ali Bougatef
Keyword(s):  
Antioxidant Activities ◽  
Muscle Protein ◽  
Protein Hydrolysate ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Converting Enzyme ◽  
Angiotensin I ◽  
Angiotensin I Converting Enzyme ◽  
Ace Inhibitory

The present study investigated angiotensin-I-converting enzyme (ACE) inhibitory and antioxidant activities of barbel muscle protein hydrolysate prepared with Alcalase. The barbel muscle protein hydrolysate displayed a high ACE inhibitory activity (CI50=0.92 mg/mL). The antioxidant activities of protein hydrolysate at different concentrations were evaluated using variousin vitroantioxidant assays, including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical method and reducing power assay. The barbel muscle protein hydrolysate exhibited an important radical scavenging effect and reducing power. These results obtained byin vitrosystems obviously established the antioxidant potency of barbel hydrolysate to donate electron or hydrogen atom to reduce the free radical. Furthermore, these bioactive substances can be exploited into functional foods or used as source of nutraceuticals.

Anti-inflammatory and antioxidant activities, functional properties and mutagenicity studies of protein and protein hydrolysate obtained from Prosopis alba seed flour

2014 ◽  
Vol 161 ◽  
pp. 391-399 ◽  
Author(s):  
Florencia Cattaneo ◽  
Jorge Esteban Sayago ◽  
María Rosa Alberto ◽  
Iris Catiana Zampini ◽  
Roxana Mabel Ordoñez ◽  
...  
Keyword(s):  
Functional Properties ◽  
Antioxidant Activities ◽  
Protein Hydrolysate ◽  
Prosopis Alba ◽  
Seed Flour ◽  
Anti Inflammatory

Germinated Brown Rice Enhances Antioxidant Activities and Immune Functions in Aged Mice

2013 ◽  
Vol 90 (6) ◽  
pp. 601-607 ◽  
Author(s):  
Fengfeng Wu ◽  
Haiying Chen ◽  
Na Yang ◽  
Xiang Duan ◽  
Zhengyu Jin ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Brown Rice ◽  
Immune Functions ◽  
Germinated Brown Rice ◽  
Aged Mice
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