Gracilaria edulis exhibit antiproliferative activity against human lung adenocarcinoma cell line A549 without causing adverse toxic effect in vitro and in vivo

2016 ◽  
Vol 7 (2) ◽  
pp. 1155-1165 ◽  
Author(s):  
Ravi Sakthivel ◽  
Samuthirapandi Muniasamy ◽  
Govindaraju Archunan ◽  
Kasi Pandima Devi
Keyword(s):  
Lung Adenocarcinoma ◽  
Antiproliferative Activity ◽  
Human Lung ◽  
Adenocarcinoma Cell Line ◽  
Gracilaria Edulis ◽  
Adenocarcinoma Cell ◽  
Lung Adenocarcinoma Cell Line ◽  
Human Lung Adenocarcinoma Cell

In the present study, the antiproliferative potential of various solvent extracts ofGracilaria edulis(GE) was tested against various cancer cell lines.

CHARACTERISATION OF PLATELET AGGREGATING MATERIAL EXTRACTED FROM HUMAN LUNG ADENOCARCINOMA CELL LINE WHICH METASTASIZED IN NUDE MICE S,C, IMPLANTATION

1987 ◽  
Author(s):  
H Inufusa ◽  
N Sagara ◽  
K Nakano ◽  
M Yasutomi
Keyword(s):  
Platelet Aggregation ◽  
Lung Adenocarcinoma ◽  
Human Lung ◽  
Human Cancer ◽  
Metastatic Potential ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Lung Adenocarcinoma Cell Line ◽  
Aggregation Activity ◽  
Human Lung Adenocarcinoma Cell

It has been reported in animal experimental system that Platelet Aggregating Material (PAM) of cancer cell play important role in cancer metastasis. Many human cancer cell lines has been also studied the platelet aggregation activity of PAM. But correlation between the platelet aggregation activity and metastatic potential of human cancer cells were usually unkncwn. We established a human lung adenocarcinoma cell line KUM-LK-2 which produce spontaneous lung metastasis when cells implanted into subcutaneous of nude mice. PAM was extracted from KUM-LK-2 cells following the method of D.Mbhanty and P.Hilgard. Platelet aggregation study of PAM was performed by human Heparinized platelet rich plasma useing NIKO Hematracer PACT2D. Character of PAM were examined by physical and chemical treatment. KUM-LK-2 PAM shew 80% of platelet aggregation in maximum after 150 sec lag time, and aggregation was not found by Citrated PRP.It is concluded that PAM is high moleculer protein and contain Phospholipid and aggregation activity is concerning with ATP composition of platelet. PAM dose not contain Fibrinogen and Sialic acid, and not concern with Throrriboxan composition. This study is first case of platelet aggregation activity of PAM extracted human canoer cells which contain metastatic potential.

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