Oral bioavailability and sex specific tissue partitioning of quantum dots in fathead minnows, Pimephales promelas

2015 ◽  
Vol 2 (6) ◽  
pp. 583-593
Author(s):  
C. M. Lavelle ◽  
J. H. Bisesi ◽  
M. A. Hahn ◽  
K. J. Kroll ◽  
T. Sabo-Attwood ◽  
...  

Quantum dots with different surface chemistries display a sex-specific tissue distribution following oral exposure in fathead minnows, an agastric fish.

Author(s):  
Richard L. Leino ◽  
Jon G. Anderson ◽  
J. Howard McCormick

Groups of 12 fathead minnows were exposed for 129 days to Lake Superior water acidified (pH 5.0, 5.5, 6.0 or 6.5) with reagent grade H2SO4 by means of a multichannel toxicant system for flow-through bioassays. Untreated water (pH 7.5) had the following properties: hardness 45.3 ± 0.3 (95% confidence interval) mg/1 as CaCO3; alkalinity 42.6 ± 0.2 mg/1; Cl- 0.03 meq/1; Na+ 0.05 meq/1; K+ 0.01 meq/1; Ca2+ 0.68 meq/1; Mg2+ 0.26 meq/1; dissolved O2 5.8 ± 0.3 mg/1; free CO2 3.2 ± 0.4 mg/1; T= 24.3 ± 0.1°C. The 1st, 2nd and 3rd gills were subsequently processed for LM (methacrylate), TEM and SEM respectively.Three changes involving chloride cells were correlated with increasing acidity: 1) the appearance of apical pits (figs. 2,5 as compared to figs. 1, 3,4) in chloride cells (about 22% of the chloride cells had pits at pH 5.0); 2) increases in their numbers and 3) increases in the % of these cells in the epithelium of the secondary lamellae.


1983 ◽  
Vol 17 (1) ◽  
pp. 47-63 ◽  
Author(s):  
James A. Zischke ◽  
John W. Arthur ◽  
Kathleen J. Nordlie ◽  
Roger O. Hermanutz ◽  
Douglas A. Standen ◽  
...  

Development ◽  
1993 ◽  
Vol 117 (4) ◽  
pp. 1409-1420 ◽  
Author(s):  
R. Moore ◽  
F.S. Walsh

The spatiotemporal distribution of M-cadherin mRNA has been determined by in situ hybridization in the mouse embryo and in adult skeletal muscle following experimental regeneration and denervation. M-cadherin mRNA is highly tissue specific and is found only in developing skeletal muscle. In contrast, N-cadherin mRNA has a broader tissue distribution in the embryo, being found on both neural elements and skeletal and cardiac muscle. M-cadherin is expressed in the myotomes shortly after they form, along with the myogenic regulatory factor myogenin. M-cadherin is expressed in muscles derived from the myotomes and is detected in forelimb bud precursor cells at embryonic day 11.5. In the latter case M-cadherin expression appears co-ordinately with that of myogenin and cardiac alpha-actin. Shortly before birth, M-cadherin expression is down regulated. M-cadherin can, however, be re-expressed following experimental regeneration of skeletal muscle. Here M-cadherin is transiently expressed on regenerating myoblasts but not myotubes. Following muscle denervation no evidence was found for re-expression of M-cadherin under conditions where there was strong expression of the nicotinic acetylcholine receptor on myofibres. The highly specific tissue distribution and unique developmental profile distinguishes M-cadherin from other cadherins and suggests a role in cell surface events during early myogenesis.


2004 ◽  
Vol 23 (12) ◽  
pp. 2900 ◽  
Author(s):  
Katherine L. Sciera ◽  
J. Jeffery Isely ◽  
Joseph R. Tomasso ◽  
Stephen J. Klaine

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