Synthesis and catalytic, antimicrobial and cytotoxicity evaluation of gold and silver nanoparticles using biodegradable, Π-conjugated polyamic acid

2015 ◽  
Vol 2 (5) ◽  
pp. 518-527 ◽  
Author(s):  
Victor M. Kariuki ◽  
Idris Yazgan ◽  
Ali Akgul ◽  
Andrzej Kowal ◽  
Magdalena Parlinska ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Silver Nanoparticles ◽  
Amic Acid ◽  
Simple Synthesis ◽  
Gold And Silver Nanoparticles ◽  
Polyamic Acid ◽  
Biodegradable Poly

We hereby report a rapid and simple synthesis of silver (AgNPs) and gold nanoparticles (AuNPs) using conductive, electroactive and biodegradable poly(amic)acid (PAA) polymer as both the reductant and stabilizer.

scholarly journals Isolation and Optimization of Culture Conditions of Thraustochytrium kinnei for Biomass Production, Nanoparticle Synthesis, Antioxidant and Antimicrobial Activities

2021 ◽  
Vol 9 (6) ◽  
pp. 678
Author(s):  
Kaliyamoorthy Kalidasan ◽  
Nabikhan Asmathunisha ◽  
Venugopal Gomathi ◽  
Laurent Dufossé ◽  
Kandasamy Kathiresan
Keyword(s):  
Antimicrobial Activity ◽  
Gold Nanoparticles ◽  
Silver Nanoparticles ◽  
Biomass Production ◽  
Petroleum Ether Extract ◽  
Antimicrobial Activities ◽  
Culture Conditions ◽  
Gold And Silver Nanoparticles ◽  
Optimization Of Culture Conditions ◽  
Antioxidant And Antimicrobial Activities

This work deals with the identification of a predominant thraustochytrid strain, the optimization of culture conditions, the synthesis of nanoparticles, and the evaluation of antioxidant and antimicrobial activities in biomass extracts and nanoparticles. Thraustochytrium kinnei was identified as a predominant strain from decomposing mangrove leaves, and its culture conditions were optimized for maximum biomass production of 13.53 g·L−1, with total lipids of 41.33% and DHA of 39.16% of total fatty acids. Furthermore, the strain was shown to synthesize gold and silver nanoparticles in the size ranges of 10–85 nm and 5–90 nm, respectively. Silver nanoparticles exhibited higher total antioxidant and DPPH activities than gold nanoparticles and methanol extract of the strain. The silver nanoparticles showed higher antimicrobial activity than gold nanoparticles and petroleum ether extract of the strain. Thus, Thraustochytrium kinnei is proven to be promising for synthesis of silver nanoparticles with high antioxidant and antimicrobial activity.

scholarly journals Green ultrasonic synthesis, Characterization and Antibacterial activity of Silver and Gold Nanoparticles mediated by Ganoderma lucidum extract

2020 ◽  
Vol 4 (2) ◽  
Keyword(s):  
Gold Nanoparticles ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Metal Nanoparticles ◽  
Cytotoxic Activities ◽  
Gold And Silver Nanoparticles ◽  
Silver And Gold Nanoparticles ◽  
E Coli ◽  
Inhibition Zones

Metal nanoparticles possess an extensive scientific and technological significance due to their unique physiochemical properties and their potential applications in different fields like medicine. Silver and gold nanoparticles have shown to have antibacterial and cytotoxic activities. Conventional methods used in the synthesis of the metal nanoparticles involve use of toxic chemicals making them unsuitable for use in medical field. In our continued effort to explore for simple and eco-friendly methods to synthesize the metal nanoparticles, we here describe synthesis and characterization of gold and silver nanoparticles using Gonaderma lucidum, wild non-edible medicinal mushroom. G. lucidum mushroom contain bioactive compounds which can be involved in the reduction, capping and stabilization of the nanoparticles. Antibacterial activity analysis was done on E. coli and S. aureus. The synthesis was done on ultrasonic bath. Characterization of the metal nanoparticles was done by UV-VIS., High Resolution Transmission Electron Microscope (HRTEM) and FTIR. HRTEM analysis showed that both silver and gold nanoparticles were spherical in shape with an average size of 15.82±3.69 nm for silver and 24.73±5.124nm for gold nanoparticles (AuNPs). FTIR analysis showed OH and -C=C- stretching vibrations, an indication of presence of functional groups of biomolecules capping both gold and silver nanoparticles. AgNPs showed inhibition zones of 15.5±0.09mm and 13.3±0.14mm while AuNPs had inhibition zones of 14.510±0.35 and 13.3±0.50mm on E. coli and S. aureus respectively. The findings indicate the potential use of AgNPs and AuNPs in development of drugs in management of pathogenic bacteria.

Green Synthesis, Characterization and Cytotoxic Activity of Gold and Silver Nanoparticles Using Kaempferol-3′-sulfonate

2020 ◽  
Vol 42 (3) ◽  
pp. 440-440
Author(s):  
Xin Bin Yang Xin Bin Yang ◽  
Chun Mei Wang Chun Mei Wang ◽  
Yu Huang Yu Huang
Keyword(s):  
Gold Nanoparticles ◽  
Silver Nanoparticles ◽  
Cytotoxic Activity ◽  
Room Temperature ◽  
Water Soluble ◽  
Ftir Analysis ◽  
Spherical Nanoparticles ◽  
Gold And Silver Nanoparticles ◽  
Stabilizing Agents ◽  
Mcf 7

Gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs) were synthesized by using water-soluble Kaempferol–3′–sulfonate acid sodium (KS) alone as the reducing agent. The UV-vis spectra confirmed the formation of the ks-AuNPs and ks-AgNPs which were stable for up to 3 months without any other stabilizing agents at room temperature. The TEM studies exhibited monodispersed and mainly spherical nanoparticles with the size in the range of 15-30 nm and 20-50 nm for ks-AuNPs and ks-AgNPs, respectively. The XRD revealed crystallinity of nanoparticles. The chemical state of Au and Ag on the surface of nanoparticles was analyzed by XPS. The FTIR analysis indicated that the hydroxyl of KS were responsible for the reduction of Au3+ and Ag+ to ks-AuNPs and ks-AgNPs, respectively. Cytotoxic activity of ks-AuNPs and ks-AgNPs on MCF-7 cells is higher than the KS.

Morphological analysis of the antimicrobial action of silver and gold nanoparticles stabilized with ceftriaxone on Escherichia coli using atomic force microscopy

2014 ◽  
Vol 38 (11) ◽  
pp. 5633-5640 ◽  
Author(s):  
Muhammad Raza Shah ◽  
Shujat Ali ◽  
Muhammad Ateeq ◽  
Samina Perveen ◽  
Shakil Ahmed ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gold Nanoparticles ◽  
Atomic Force Microscopy ◽  
Silver Nanoparticles ◽  
Morphological Analysis ◽  
Antimicrobial Action ◽  
Antibacterial Action ◽  
Gold And Silver Nanoparticles ◽  
Force Microscopy ◽  
Atomic Force

Monitoring the antibacterial action of gold and silver nanoparticlesviaAFM.

scholarly journals SERS Characterization of the Indocyanine-Type Dye IR-820 on Gold and Silver Nanoparticles in the Near Infrared

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Tatiana B. V. Neves ◽  
Gustavo F. S. Andrade
Keyword(s):  
Gold Nanoparticles ◽  
Silver Nanoparticles ◽  
Raman Spectrum ◽  
High Performance ◽  
Near Infrared ◽  
Cyanine Dyes ◽  
Metallic Surface ◽  
Gold And Silver Nanoparticles ◽  
Biological Compatibility

The Raman spectrum of the indocyanine-type dye IR-820 has been assigned for both solid and solution. SERS spectra of IR-820 on both silver and gold nanoparticles suspensions excited at 1064 nm were obtained. AgNPs allowed the detection of the dye through SERS down to 0.1 micromoles per liter; for the AuNPs the lowest concentration of the dye detectable was 10 micromoles per liter. Changes in the SERS relative intensities compared to the Raman spectrum in solution are subtle, mostly due to the preresonance effect of the dye. However, a perpendicular orientation relative to the metallic surface was inferred for the dye on both AgNPs and AuNPs. The easily distinguishable SERS spectra of the dye excited at 1064 nm, together with the high biological compatibility of cyanine dyes, are both indicative that IR-820 could be used as a high-performance probe molecule for SERS.

A Study on Understanding Potential Gold and Silver Nanoparticle : An Overview

2020 ◽  
pp. 2150009
Author(s):  
Shadeeb Hossain
Keyword(s):  
Gold Nanoparticles ◽  
Silver Nanoparticles ◽  
Optical Property ◽  
Severe Acute Respiratory Syndrome ◽  
Silver Nanoparticle ◽  
Diagnostic Tool ◽  
Transmission Rate ◽  
Electrochemical Techniques ◽  
Gold And Silver Nanoparticles ◽  
Rapid Transmission

This paper highlights on the coronavirus outbreak caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). At the time of writing this paper, there has been over 6 million confirmed cases worldwide. It is a person–person transmittable infection but there have been cases of asymptomatic carriers. Hence, development of an effective biosensing diagnostic tool can curb its rapid transmission rate. The first part of the paper highlights on the SARS-CoV-2 structure and its resemblance to SARS-CoV. The second part of the paper analyzes on the potential application of gold and silver nanoparticles to generate a red shift that had enhanced the calorimetric property of the MERS-CoV analysis due to transition in its optical property. Other electrochemical techniques that utilized the application of gold nanoparticles are also reviewed. Gold and silver nanoparticles (AuNP and Ag NP) can accelerate the sensitivity upon electrodeposition on the diagnostic tool.

Sensitivity Enhancement in the Colorimetric/Spectroscopic Determination of Lysozyme Concentration in Nanomolar Level with Colloidal Citrate Capped Au@Ag Core-Shell Nanoparticles

2020 ◽  
Vol 10 ◽  
Author(s):  
Tapanendu Kamilya ◽  
Amit Kumar Bhunia ◽  
Pijus K. Samanta ◽  
Satyajit Saha ◽  
Rudra N. Mondal ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Silver Nanoparticles ◽  
Core Shell ◽  
Shell Nanoparticles ◽  
Gold And Silver Nanoparticles ◽  
Gold Silver ◽  
Lysozyme Concentration ◽  
Core Shell Nanoparticles ◽  
Silver Core ◽  
Higher Sensitivity

Background: Lysozyme level in body fluids is a significant indicator of various diseases. Cheap and simple colorimetric detection of lysozyme, in biological sample, by gold and silver nanoparticles is a field of interest of nanoparticle research for more than a decade Objective: We report here an attempt to improve the sensitivity part of the colorimetric lysozyme detection process by using citrate capped gold-silver core-shell nanoparticles without any functionalization Methods: Performance of gold-silver core-shell nanoparticles in determining of lysozyme concentration in water has been done using colorimetric/spectroscopic technique. Studies have also been done with gold nanoparticles, silver nanoparticles and mixed colloidal suspension of gold and silver nanoparticles, for comparison. Selectivity study has been done through spectroscopic analysis, red-green-blue colour component analysis and fractal dimension analysis of the nanoparticles interacted with several low and high isoelectric proteins Results: Gold-silver core-shell nanoparticles showed higher sensitivity for a wider range of lysozyme concentration compared to gold and silver nanoparticles prepared by us or reported in literatures. Gold nanoparticles showed higher sensitivity compared to the core-shell nanoparticles, but for a narrow concentration range of lysozyme. For silver nanoparticles and the mixed nanoparticle system, both sensitivity and range of determination of lysozyme concentration were much smaller compared to the core-shell nanoparticles. Core-shell nanoparticles showed better selectivity compared to gold nanoparticles in identifying aquatic solution of lysozyme from that of other proteins Conclusion: Gold-silver core-shell nanoparticles have higher sensitivity in determining wide range of lysozyme concentration in water compared to gold and silver nanoparticles reported in literatures

FOLIC ACID-FUNCTIONALIZED GOLD AND SILVER NANOPARTICLES: THEIR CYTOTOXIC EFFECT ON CANCEROUS MYELOID CELLS WITH MICROWAVE IRRADIATION

Nano LIFE ◽  
2013 ◽  
Vol 03 (02) ◽  
pp. 1350003 ◽  
Author(s):  
SEYEDHOSSEIN HEKMATIMOGHADDAM ◽  
ALI JEBALI ◽  
MANDANA DARGAHI
Keyword(s):  
Gold Nanoparticles ◽  
Silver Nanoparticles ◽  
Folic Acid ◽  
Microwave Irradiation ◽  
Cytotoxic Effect ◽  
Cell Cytotoxicity ◽  
Functionalized Nanoparticles ◽  
Gold And Silver Nanoparticles ◽  
Normal Cells ◽  
Functionalized Gold Nanoparticles

Introduction: Metal nanoparticles such as gold and silver nanoparticles have attracted much interest during the last decades for their special chemical and physical properties. Gold and silver nanoparticles can be functionalized with active biologic moieties like antibodies, drugs and chemicals, enabling them to react with specific cells. Furthermore, penetration and cytotoxic effects of nanoparticles can be increased by electromagnetic waves such as infrared, ultraviolet, radiofrequency and microwave. Aim: The aim of this study was to evaluate the rate of cell cytotoxicity induced by folic acid-functionalized gold and silver nanoparticles with and without microwave irradiation on cancer cells from patients with acute myeloid leukemia (AML). Method: Patients with known AML (M1, M2, M3 and M4), all recently diagnosed by histopathology, special stains and immunohistochemistry, and 4 normal persons were enrolled in the study. The blood mononuclear cell fraction was separated, so that the final concentration of neoplastic myeloid cells and normal mononuclear cells in each tube was adjusted to about 400 cells/μL. For preparation of folate-functionalized gold and silver nanoparticles, folic acid was dissolved in deionized water, added to 1 mM HAuCl4 and 1 mM AgNO3 solution, and incubated at 50°C for 8 h. Scanning electron micrographs, ultraviolet-visible spectrophotometer and Fourier transform infrared (FTIR) were used for confirmation of the synthesis of functionalized nanoparticles. After preparation, nanoparticles were added to cancerous and normal cell suspensions, and then incubated at 37°C for 1 h. Another experiment was carried out in the same way but with exposure to microwave irradiation for 10 s so that its temperature reached at 50°C, and then incubated at 37°C for 1 h, after which cell cytotoxicity was evaluated with MTT test. All of the tests were duplicated, and paired t-test was used to compare the mean absorbance read-out in each of the above-mentioned groups of wells. Results: The sizes of functionalized gold and silver nanoparticles were approximately 25 nm to 32 nm. After synthesis of functionalized nanoparticles, the tubes containing HAuCl4 turned to red color, and the peak absorbance for gold nanoparticles was at 520 nm. For AgNo3 , it turned to yellow color with a peak absorbance at 420 nm. FTIR test showed connection of folic acid moieties to gold and silver surfaces. This study showed that functionalized gold nanoparticles were more toxic than functionalized silver nanoparticles on cancer and normal cells. Also, microwave irradiation was more synergic with functionalized gold nanoparticles. Furthermore, the most effectiveness score was 2.87 for functionalized silver nanoparticles without microwave irradiation and the minimum effectiveness score was 2.20 for functionalized silver nanoparticles with microwave. Conclusion: This study clearly demonstrated that although functionalized gold nanoparticles have high toxicity to cells, but silver nanoparticles without microwave irradiation are more effective because of less cytotoxic effect on normal cells.

scholarly journals Knowledge and awareness on the use of gold and silver nanoparticles in endodontics practice - A survey

2020 ◽  
Vol 11 (SPL3) ◽  
pp. 1780-1785
Author(s):  
Jaya vabushana V ◽  
Lakshmi T ◽  
Gayathri R
Keyword(s):  
Gold Nanoparticles ◽  
Silver Nanoparticles ◽  
Dental Caries ◽  
Web Search ◽  
Absolute Number ◽  
Bond Quality ◽  
Gold And Silver Nanoparticles ◽  
Dispersed Particles ◽  
Silver Nps ◽  
The Impact

To make mindfulness and teach professionals about the impact of consolidating gold and silver NPs on the shear bond quality in endodontics. Silver and Gold nanoparticles have uncommon attributes that settle on them as great decisions as fillers for dental caries filling. They could remain in nanoscale in the nearness of appropriate stabilizers which implies little collection or bunching and consistently dispersed particles. They have high synthetic dependability and make them simple to be integrated and controlled. Biocompatibility makes them nontoxic for living beings. Some considered the nearness of nanoparticles could redirect the miniaturized scale split and viably increment the protection from the applied force to diminish the clinical disappointments because of less bond quality composite. This study will be helpful. This overview assesses the information and mindfulness on the utilization of gold and silver nanoparticles in endodontic practice. An absolute number of 20 articles were chosen—period (or) term considered for reference articles 2000 to 2020. The information for this article was gathered from web search tools like PUBMED, GOOGLE SCHOLAR, MESH, COCHRANE, SEMANTIC SCHOLAR. Through this examination, we have talked about the mindfulness utilization of gold and silver nanoparticles in endodontic practice. It makes professionals know about the benefits of gold and silver nanoparticles.

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