Biodegradation of different petroleum hydrocarbons by free and immobilized microbial consortia

2015 ◽  
Vol 17 (12) ◽  
pp. 2022-2033 ◽  
Author(s):  
Tiantian Shen ◽  
Yongrui Pi ◽  
Mutai Bao ◽  
Nana Xu ◽  
Yiming Li ◽  
...  
Keyword(s):  
Crude Oil ◽  
Petroleum Hydrocarbons ◽  
Microbial Consortia ◽  
Biodegradation Rate

The biodegradation rate of crude oil by semi-coke immobilized microbial consortia was higher than that by free microbial consortia.

scholarly journals Evolution of the Microbioal Population in a Polluted Soil with Petroleum Hydrocarbons

1970 ◽  
Vol 66 (2) ◽  
Author(s):  
Mariana MARINESCU ◽  
M. DUMITRU ◽  
Anca LĂCĂTUŞU ◽  
Gabriela MIHALACHE
Keyword(s):  
Crude Oil ◽  
Natural Product ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Environmental Problems ◽  
Polluted Soil ◽  
Total Petroleum Hydrocarbons ◽  
Biodegradation Rate ◽  
Green House ◽  
Bacterial Inoculum

Crude oil bioremediation of soils is limited by the bacteria activity in degrading the spills hydrocarbons. The aim of this study is to enhance the bioremediation of soils polluted with crude oil by adding the natural biodegradable product and bacterial inoculum. Biodegradation was quantified by total petroleum hydrocarbons (TPH) analyses. Petroleum hydrocarbon pollution is one of the main environmental problems, not only by the important amounts released but also because of their toxicity. The main objective of this work is to accelerate the biodegradation processes. The enhancement of petroleum hydrocarbons degradation was achieved under natural product treatment and bacterial inoculum. The bacterial inoculum was used to enrich indigenous microbes to enhance biodegradation rate in the green house experiment.

Efficiency and Kinetics of Total Petroleum Hydrocarbons (TPHs) Removal from Crude Oil Polluted Arable Soil using Palm Bunch Ash and Tween 80

2021 ◽  
Author(s):  
Godwin James Udo ◽  
Nnanake-Abasi O. Offiong ◽  
Alfreda Nwadinigwe ◽  
Clement O. Obadimu ◽  
Aniedi E. Nyong ◽  
...  
Keyword(s):  
Crude Oil ◽  
Petroleum Hydrocarbons ◽  
Tween 80 ◽  
Arable Soil ◽  
Total Petroleum Hydrocarbons ◽  
Kinetics Of ◽  
Palm Bunch

scholarly journals Efficiency and Kinetics of Assam Crude Oil Degradation by Pseudomonas Aeruginosa AKS1 and Bacillus Sp. AKS2

2021 ◽  
Author(s):  
Bobby Chettri ◽  
Ningombam Anjana Singha ◽  
Arvind Kumar Singh
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Crude Oil ◽  
Liquid Medium ◽  
Half Life ◽  
Liquid Culture ◽  
Oil Degradation ◽  
Biodegradation Rate ◽  
Emulsification Index ◽  
Crude Oil Degradation ◽  
Kinetics Of

Abstract We report kinetics of Assam crude oil degradation by Pseudomonas aeruginosa AKS1 and Bacillus sp. AKS2, both isolated from Assam refinery sediments. The isolates exhibited appreciable degrees of hydrophobicity, emulsification index and biosurfactant production. Crude oil degradation efficiency of isolates was assessed in (1) liquid medium amended with 1% v/v crude oil and (2) microcosm sediments (125 mg crude oil/ 10 g sand). In liquid culture, the biodegradation rate (k) and half-life (t1/2) values were found to be 0.0383 day -1 and 18.09 days for P. aeruginosa AKS1, and 0.0204 day -1 and 33.97 days in case of Bacillus sp. AKS2. In microcosm sand sediments, the estimated biodegradation rate (k) and half-life (t 1/2) values were 0.0138 day -1 and 50 days for P. aeruginosa AKS1, and 0.0113 day -1 and 61.34 days in case of Bacillus sp. AKS2. The level of nutrient treatment in microcosm sand sediment was 125 µg N & 62.5 µg P/g sediment in case of P. aeruginosa AKS1 and 375 µg N & 37.5 µg P/g sediment in case of Bacillus sp. AKS2. In microcosms without inorganic nutrients, biodegradation rate (k) and half-life (t1/2) values were found to be 0.0069 day -1 and 100 days for P. aeruginosa AKS1 and for Bacillus sp. AKS2, the respective values were found to be 0.0046 day -1 and 150.68 days. Our data provides important information for predictive hydrocarbon degradation in liquid medium and contaminated sediments.

Chromatographic study of crude oil

2020 ◽  
pp. 47-50
Author(s):  
T.S. Idrisov ◽  
Keyword(s):  
Crude Oil ◽  
Aromatic Compounds ◽  
Petroleum Hydrocarbons ◽  
Chromatographic Analysis ◽  
Scientific Research ◽  
Chromatographic Study ◽  
Column Temperature ◽  
Tof Ms

The paper deals with the methods of preparation of crude oil samples and chromatographic analysis of petroleum hydrocarbons (alkanes, monoaromatic compounds, policyclic aromatic compounds), as well as the results of the analysis (chromatograms of the samples and concentrates of hydrocarbons). The analysis have been carried out on Shimadzu GC 2010-plus and PEGASUS 4D GCxGC TOF-MS chromatographs. In the analysis of alkanes, monoaromatic and policyclic aromatic compounds the column temperature comprised 60−330 оС, 40−120 оС and 70−300 оС correspondingly. Silicagel of 100−200 mehs size was used as a sorbent. Developed methods may be applied for the analytical purposes in petrochemistry and corresponding scientific research surveys as well.

NOVEL STRAINS OF MICROORGANISMS DEGRADING PETROLEUM HYDROCARBONS

2020 ◽  
pp. 311-313
Author(s):  
N. Tyschenko ◽  
D. Ivasenko ◽  
А. Kosov ◽  
D. Rybkin ◽  
Е. Lukjanova ◽  
...  
Keyword(s):  
Crude Oil ◽  
Bottom Sediments ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Biological Products

Novel strains of hydrocarbons degrading microorganisms were isolated from oil contaminated soil and bottom sediments and identified. Isolates oxidize components of crude oil in presence of oxygen and can be used as a part of commercial biological products for oil destruction.

scholarly journals Production of CO2 in crude oil bioremediation in clay soil

2005 ◽  
Vol 48 (spe) ◽  
pp. 249-255 ◽  
Author(s):  
Sandro José Baptista ◽  
Magali Christe Cammarota ◽  
Denize Dias de Carvalho Freire
Keyword(s):  
Crude Oil ◽  
Petroleum Hydrocarbons ◽  
Clay Soil ◽  
Fixed Bed ◽  
Bacterial Count ◽  
Fixed Bed Reactor ◽  
Total Petroleum Hydrocarbons ◽  
Co2 Production ◽  
Oil And Grease ◽  
Before And After

The aim of the present work was to evaluate the biodegradation of petroleum hydrocarbons in clay soil a 45-days experiment. The experiment was conducted using an aerobic fixed bed reactor, containing 300g of contaminated soil at room temperature with an air rate of 6 L/h. The growth medium was supplemented with 2.5% (w/w) (NH4)2SO4 and 0.035% (w/w) KH2PO4. Biodegradation of the crude oil in the contaminated clay soil was monitored by measuring CO2 production and removal of organic matter (OM), oil and grease (OandG), and total petroleum hydrocarbons (TPH), measured before and after the 45-days experiment, together with total heterotrophic and hydrocarbon-degrading bacterial count. The best removals of OM (50%), OandG (37%) and TPH (45%) were obtained in the bioreactors in which the highest CO2 production was achieved.

Toxicity Assessment of Groundwater Contaminated by Petroleum Hydrocarbons at a Well-Characterized, Aged, Crude Oil Release Site

2018 ◽  
Vol 52 (21) ◽  
pp. 12172-12178 ◽  
Author(s):  
Jennifer T. McGuire ◽  
Isabelle M. Cozzarelli ◽  
Barbara A. Bekins ◽  
Hannah Link ◽  
Dalma Martinović-Weigelt
Keyword(s):  
Crude Oil ◽  
Petroleum Hydrocarbons ◽  
Release Site ◽  
Toxicity Assessment
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