scholarly journals Interaction of europium and curium with alpha-amylase

2016 ◽  
Vol 45 (21) ◽  
pp. 8724-8733 ◽  
Author(s):  
Astrid Barkleit ◽  
Anne Heller ◽  
Atsushi Ikeda-Ohno ◽  
Gert Bernhard
Keyword(s):  
Strong Interaction ◽  
Digestive Enzyme ◽  
Alpha Amylase ◽  
Batch Sorption ◽  
Spectroscopic Titration

Batch sorption experiments, potentiometric and spectroscopic titration investigations revealed a fast and strong interaction of Eu(iii) and Cm(iii) with the digestive enzyme α-amylase.

Endocrine secretion of alpha-amylase by the pancreas

1981 ◽  
Vol 241 (2) ◽  
pp. G170-G175 ◽  
Author(s):  
K. Miyasaka ◽  
S. S. Rothman
Keyword(s):  
Acinar Cell ◽  
Digestive Enzyme ◽  
Alpha Amylase ◽  
Gastrointestinal Hormone ◽  
Amylase Secretion ◽  
Amylase Release ◽  
Applied Dose ◽  
Rabbit Pancreas ◽  
Order Of Magnitude ◽  
Endocrine Secretion

The effect of the gastrointestinal hormone, cholecystokinin-pancreozymin (CCK-PZ), on the flux of alpha-amylase across the basolateral surface of the pancreas into interstitial fluid was measured by following its appearance in the medium bathing whole rabbit pancreas in organ culture. CCK-PZ increased the rate of amylase release by about an order of magnitude for the maximum applied dose. The response was only observed at concentrations of CCK-PZ that were supramaximal for ductal enzyme secretion (320 pmol/l to 10 nmol/l). Over this range, amylase secretion into the bath varied widely with dose, whereas that into the duct remained relatively unchanged. These observations, in conjunction with others, suggest that the acinar cell, and not the duct system, is the direct source of this amylase and that there is a natural secretion of digestive enzyme from the acinar cell in the endocrine direction that is augmented by CCK-PZ or a homologous peptide.

scholarly journals Jasmonate-independent regulation of digestive enzyme activity in the carnivorous butterwort Pinguicula × Tina

2020 ◽  
Vol 71 (12) ◽  
pp. 3749-3758 ◽  
Author(s):  
Ondřej Kocáb ◽  
Jana Jakšová ◽  
Ondřej Novák ◽  
Ivan Petřík ◽  
René Lenobel ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Enzyme Activities ◽  
Digestive Enzymes ◽  
Defense Mechanisms ◽  
Prey Capture ◽  
Digestive Enzyme ◽  
Carnivorous Plants ◽  
Alpha Amylase ◽  
Jasmonate Signaling ◽  
Digestive Enzyme Activities

Abstract Carnivorous plants within the order Caryophyllales use jasmonates, a class of phytohormone, in the regulation of digestive enzyme activities. We used the carnivorous butterwort Pinguicula × Tina from the order Lamiales to investigate whether jasmonate signaling is a universal and ubiquitous signaling pathway that exists outside the order Caryophyllales. We measured the electrical signals, enzyme activities, and phytohormone tissue levels in response to prey capture. Mass spectrometry was used to identify proteins in the digestive secretion. We identified eight enzymes in the digestive secretion, many of which were previously found in other genera of carnivorous plants. Among them, alpha-amylase is unique in carnivorous plants. Enzymatic activities increased in response to prey capture; however, the tissue content of jasmonic acid and its isoleucine conjugate remained rather low in contrast to the jasmonate response to wounding. Enzyme activities did not increase in response to the exogenous application of jasmonic acid or coronatine. Whereas similar digestive enzymes were co-opted from plant defense mechanisms among carnivorous plants, the mode of their regulation differs. The butterwort has not co-opted jasmonate signaling for the induction of enzyme activities in response to prey capture. Moreover, the presence of alpha-amylase in digestive fluid of P. × Tina, which has not been found in other genera of carnivorous plants, might indicate that non-defense-related genes have also been co-opted for carnivory.

Alpha-Amylase/Trypsin Inhibitors, novel activators of innate immunity in ex-vivo tissue explants

2013 ◽  
Vol 51 (08) ◽  
Author(s):  
V Zevallos ◽  
P Olinga ◽  
Y Junker ◽  
PB Tung ◽  
N Volz ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Ex Vivo ◽  
Trypsin Inhibitors ◽  
Alpha Amylase ◽  
Tissue Explants ◽  
Ex Vivo Tissue
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