Antitumor properties of platinum(iv) prodrug-loaded silk fibroin nanoparticles

2015 ◽  
Vol 44 (30) ◽  
pp. 13513-13521 ◽  
Author(s):  
A. Abel Lozano-Pérez ◽  
Ana L. Gil ◽  
Sergio A. Pérez ◽  
Natalia Cutillas ◽  
Hajo Meyer ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
High Cell ◽  
Cell Selectivity ◽  
Silk Fibroin Nanoparticles ◽  
Antitumor Properties

We have successfully generated silk nanoparticles loaded with the hydrophobic platinum(iv) antitumor prodrug PtBz achieving high cell selectivity factors.

scholarly journals Silk fibroin nanoparticles enhance quercetin immunomodulatory properties in DSS-induced mouse colitis

2021 ◽  
pp. 120935
Author(s):  
Patricia Diez-Echave ◽  
Antonio Jesús Ruiz-Malagón ◽  
José Alberto Molina-Tijeras ◽  
Laura Hidalgo-García ◽  
Teresa Vezza ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Silk Fibroin Nanoparticles ◽  
Immunomodulatory Properties

scholarly journals Formulation, characterization, and cellular toxicity assessment of tamoxifen-loaded silk fibroin nanoparticles in breast cancer

Drug Delivery ◽  
2021 ◽  
Vol 28 (1) ◽  
pp. 1626-1636
Author(s):  
Afrasim Moin ◽  
Shahid Ud Din Wani ◽  
Riyaz Ali Osmani ◽  
Amr S. Abu Lila ◽  
El-Sayed Khafagy ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Silk Fibroin ◽  
Toxicity Assessment ◽  
Cellular Toxicity ◽  
Silk Fibroin Nanoparticles

Targeted Delivery System Based on Gemcitabine-Loaded Silk Fibroin Nanoparticles for Lung Cancer Therapy

2017 ◽  
Vol 9 (37) ◽  
pp. 31600-31611 ◽  
Author(s):  
Fatemeh Mottaghitalab ◽  
Melika Kiani ◽  
Mehdi Farokhi ◽  
Subhas C. Kundu ◽  
Rui L. Reis ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Therapy ◽  
Silk Fibroin ◽  
Delivery System ◽  
Targeted Delivery ◽  
Silk Fibroin Nanoparticles ◽  
Lung Cancer Therapy ◽  
Targeted Delivery System

Bioconjugation of Neutral Protease on Silk Fibroin Nanoparticles and Application in the Controllable Hydrolysis of Sericin

2011 ◽  
Vol 59 (18) ◽  
pp. 10298-10302 ◽  
Author(s):  
Lin Zhu ◽  
Ren-Ping Hu ◽  
Hai-Yan Wang ◽  
Yuan-Jing Wang ◽  
Yu-Qing Zhang
Keyword(s):  
Silk Fibroin ◽  
Neutral Protease ◽  
Silk Fibroin Nanoparticles ◽  
Hydrolysis Of

scholarly journals Silk fibroin nanoparticles dyeing indocyanine green for imaging-guided photo-thermal therapy of glioblastoma

Drug Delivery ◽  
2018 ◽  
Vol 25 (1) ◽  
pp. 364-375 ◽  
Author(s):  
He-Lin Xu ◽  
De-Li ZhuGe ◽  
Pian-Pian Chen ◽  
Meng-Qi Tong ◽  
Meng-Ting Lin ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Silk Fibroin ◽  
Thermal Therapy ◽  
Silk Fibroin Nanoparticles

scholarly journals Improving Anticancer Therapy with Naringenin-Loaded Silk Fibroin Nanoparticles

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 718 ◽  
Author(s):  
Marta G. Fuster ◽  
Guzmán Carissimi ◽  
Mercedes G. Montalbán ◽  
Gloria Víllora
Keyword(s):  
Silk Fibroin ◽  
Drug Loading ◽  
Drug Release Profile ◽  
Solubility In Water ◽  
Diphenyltetrazolium Bromide ◽  
Wide Range ◽  
Silk Fibroin Nanoparticles ◽  
Ea.Hy926 Cells ◽  
Cytotoxicity Effects

Naringenin (NAR), a flavonoid present in a variety of fruits, vegetables and herbs, exhibits a wide range of pharmacological effects, including anticancer activity. Nevertheless, its application in cancer therapy is limited due to its low bioavailability at the tumour site because of its poor solubility in water and slow dissolution rate. To improve the therapeutic efficacy of NAR, emergent research is looking into using nanocarriers. Silk fibroin (SF), from the Bombyx mori silkworm, is a biocompatible and biodegradable polymer with excellent mechanical properties and an amphiphilic chemistry that make it a promising candidate as a controlled release drug system. The aim of this work is to synthesize naringenin-loaded silk fibroin nanoparticles (NAR-SFNs) by dissolving the SF in the ionic liquid 1-ethyl-3-methylimidazolium acetate, using high-power ultrasounds and rapid desolvation in methanol followed by the adsorption of NAR. The NAR-SFNs were characterized by dynamic light scattering, Fourier transform infrared spectroscopy and thermogravimetric analysis. The drug loading content and encapsulation efficiency were calculated. The drug release profile best fitted a first order equation. The cytotoxicity effects of free NAR, bare silk fibroin nanoparticles (SFNs) and NAR-SFNs were assessed on HeLa and EA.hy926 cells via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results demonstrated the higher in vitro anticancer potential of synthesized NAR-SFNs than that of free NAR in HeLa cancer cells.

scholarly journals Revealing the Influence of the Degumming Process in the Properties of Silk Fibroin Nanoparticles

Polymers ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 2045 ◽  
Author(s):  
Guzmán Carissimi ◽  
A. Abel Lozano-Pérez ◽  
Mercedes G. Montalbán ◽  
Salvador D. Aznar-Cervantes ◽  
José Luis Cenis ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Nanoparticle Synthesis ◽  
Biochemical Properties ◽  
Strong Impact ◽  
Lack Of Information ◽  
Sds Page ◽  
Silk Fibroin Nanoparticles ◽  
Mean Size ◽  
Degumming Process ◽  
The Impact

Several studies have stated that the process used for sericin removal, or degumming, from silk cocoons has a strong impact in the silk fibroin integrity and consequently in their mechanical or biochemical properties after processing it into several biomaterials (e.g. fibers, films or scaffolds) but still, there is a lack of information of the impact on the features of silk nanoparticles. In this work, silk cocoons were degummed following four standard methods: autoclaving, short alkaline (Na2CO3) boiling, long alkaline (Na2CO3) boiling and ultrasounds. The resultant silk fibroin fibers were dissolved in the ionic liquid 1-ethyl-3-methylimidazolium acetate and used for nanoparticle synthesis by rapid desolvation in polar organic solvents. The relative efficiencies of the degumming processes and the integrity of the resulting fibroin fibers obtained were analyzed by mass loss, optical microscopy, thermogravimetric analysis, infrared spectroscopy and SDS-PAGE. Particle sizes and morphology were analyzed by Dynamic Light Scattering and Field Emission Scanning Electronic Microscopy. The results showed that the different treatments had a remarkable impact on the integrity of the silk fibroin chains, as confirmed by gel electrophoresis, which can be correlated with particle mean size and size distribution changes. The smallest nanoparticles (156 ± 3 nm) and the most negative Z potential (−30.2 ± 1.8 mV) were obtained with the combination of long treatment (2 h) of boiling in alkaline solution (Na2CO3 0.02 eq/L). The study confirms that parameters of the process, such as composition of the solution and time of the degumming step, must be controlled in order to reach an optimum reproducibility of the nanoparticle production.

scholarly journals Oral Bioavailability Evaluation of Celastrol-Encapsulated Silk Fibroin Nanoparticles Using an Optimized LC-MS/MS Method

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3422
Author(s):  
Shuyu Zhan ◽  
Amy Paik ◽  
Felicia Onyeabor ◽  
Baoyue Ding ◽  
Sunil Prabhu ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Oral Bioavailability ◽  
Rat Plasma ◽  
Absolute Bioavailability ◽  
Limit Of Quantification ◽  
Extraction Solvent ◽  
Liquid Liquid Extraction ◽  
Silk Fibroin Nanoparticles

Celastrol (CL), a compound isolated from Tripterygium wilfordii, possesses various bioactivities such as antitumor, anti-inflammatory and anti-obesity effects. In previous studies, we developed CL-encapsulated silk fibroin nanoparticles (CL-SFNP) with satisfactory formulation properties and in vitro cancer cytotoxicity effect. For further in vivo oral bioavailability evaluation, in this study, a simple and reliable LC-MS/MS method was optimized and validated to determine CL concentration in rat plasma. The separation of CL was performed on a C18 column (150 by 2 mm, 5 µm) following sample preparation using liquid–liquid extraction with the optimized extraction solvent of tert-butyl methylether. The assay exhibited a good linearity in the concentration range of 0.5–500 ng/mL with the lower limit of quantification (LLOQ) of 0.5 ng/mL. The method was validated to meet the requirements for bioassay with accuracy of 91.1–110.0%, precision (RSD%) less than 9.1%, extraction recovery of 63.5–74.7% and matrix effect of 87.3–101.2%. The developed method was successfully applied to the oral bioavailability evaluation of CL-SFNP. The pharmacokinetic results indicated the AUC0-∞ values of CL were both significantly (p < 0.05) higher than those for pure CL after intravenous (IV) or oral (PO) administration of equivalent CL in rats. The oral absolute bioavailability (F, %) of CL significantly (p < 0.05) increased from 3.14% for pure CL to 7.56% for CL-SFNP after dosage normalization. This study provides valuable information for future CL product development.

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