scholarly journals Physiological concentrations of albumin favor drug binding

2015 ◽  
Vol 17 (35) ◽  
pp. 22678-22685 ◽  
Author(s):  
D. Tatlidil ◽  
M. Ucuncu ◽  
Y. Akdogan
Keyword(s):  
Epr Spectroscopy ◽  
Study Drug ◽  
Drug Binding ◽  
Direct Measurements

We exploit the direct measurements of spin labeled drugs to study drug binding to/release from protein using EPR spectroscopy.

scholarly journals EPR studies of intermolecular interactions and competitive binding of drugs in a drug–BSA binding model

2016 ◽  
Vol 18 (32) ◽  
pp. 22531-22539 ◽  
Author(s):  
Y. Akdogan ◽  
M. Emrullahoglu ◽  
D. Tatlidil ◽  
M. Ucuncu ◽  
G. Cakan-Akdogan
Keyword(s):  
Intermolecular Interactions ◽  
Epr Spectroscopy ◽  
Competitive Binding ◽  
Drug Binding ◽  
Promising Technique ◽  
Binding Model ◽  
Bsa Binding

EPR spectroscopy is a very promising technique to understand the details of drug binding and competitive drug binding to proteins.

DIRECT MEASUREMENTS OF HEMOGLOBIN INTERACTIONS WITH LIPOSOMES USING EPR SPECTROSCOPY

2001 ◽  
Vol 29 (1) ◽  
pp. 5-18 ◽  
Author(s):  
Omoefe O. Abugo ◽  
Chavali Balagopalakrishna ◽  
Joseph M. Rifkind ◽  
Alan S. Rudolph ◽  
John R. Hess ◽  
...  
Keyword(s):  
Epr Spectroscopy ◽  
Direct Measurements

Recent Advances in NMR Diffusion Techniques for Studying Drug Binding

2003 ◽  
Vol 56 (9) ◽  
pp. 855 ◽  
Author(s):  
William S. Price
Keyword(s):  
Drug Development ◽  
Study Drug ◽  
Drug Binding ◽  
Model Systems ◽  
Aqueous Samples ◽  
Recent Advances ◽  
Physiological Relevance ◽  
Nmr Diffusion

Characterizing the binding of ligands to macromolecular receptors in solution is important to many areas of chemistry, biology, and nanobiotechnology, but perhaps most notably to drug development. NMR has proven to be particularly useful for such studies, but the systems studied have generally been restricted to model systems with dubious physiological relevance. This paper reviews the use of NMR diffusion measurements to study drug binding and two recent advances that enable measurements to be conducted in more sensitive higher-field NMR spectrometers in non-deuterated aqueous samples.

amTCO, a new trans-cyclooctene derivative to study drug-target interactions in cells

2021 ◽  
Vol 57 (14) ◽  
pp. 1814-1817
Author(s):  
Cécile Echalier ◽  
Anna Rutkowska ◽  
Ana Kojic ◽  
Douglas W. Thomson ◽  
Lee J. Edwards ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Drug Target ◽  
Study Drug ◽  
Drug Binding ◽  
Binding Assays ◽  
Intact Cells ◽  
In Cells

We provide a new tagging entity for click chemistry to perform improved drug binding assays in intact cells.

Direct measurements of anticrossings of the electron-nuclear energy levels in LiYF4∶Ho3+ with submillimeter EPR spectroscopy

2005 ◽  
Vol 28 (3-4) ◽  
pp. 251-265 ◽  
Author(s):  
G. S. Shakurov ◽  
M. V. Vanyunin ◽  
B. Z. Malkin ◽  
B. Barbara ◽  
R. Yu. Abdulsabirov ◽  
...  
Keyword(s):  
Epr Spectroscopy ◽  
Nuclear Energy ◽  
Energy Levels ◽  
Nuclear Energy Levels ◽  
Direct Measurements

Abstract 5655: Biophysics in the cell—CETSA to study drug binding and cellular processes in cancer therapy

2018 ◽  
Author(s):  
Ying Yu Liang ◽  
Anderson Ramos ◽  
Henriette Laursen ◽  
Olga Surova ◽  
Johan Lengqvist ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Study Drug ◽  
Drug Binding ◽  
Cellular Processes

Effect of piperacillin on morphology and viability of gram-negative bacteria

1984 ◽  
Vol 42 ◽  
pp. 218-219
Author(s):  
R. H. Liss
Keyword(s):  
Inhibitory Concentration ◽  
Cytoplasmic Membrane ◽  
Drug Binding ◽  
Gram Negative Bacteria ◽  
Bacterial Cells ◽  
Drug Induced ◽  
Penicillin Binding Proteins ◽  
Lactam Antibiotic ◽  
Drug Free ◽  
Tube Dilution

Piperacillip (PIP) is b-[D(-)-α-(4-ethy1-2,3-dioxo-l-piperzinylcar-bonylamino)-α-phenylacetamido]-penicillanate. The broad spectrum semisynthetic β-lactam antibiotic is believed to effect bactericidal activity through its affinity for penicillin-binding proteins (PBPs), enzymes on the bacterial cytoplasmic membrane that control elongation and septation during cell growth and division. The purpose of this study was to correlate penetration and binding of 14C-PIP in bacterial cells with drug-induced lethal changes assessed by microscopic, microbiologic and biochemical methods.The bacteria used were clinical isolates of Escherichia coli and Pseudomonas aeruginosa (Figure 1). Sensitivity to the drug was determined by serial tube dilution in Trypticase Soy Broth (BBL) at an inoculum of 104 organisms/ml; the minimum inhibitory concentration of piperacillin for both bacteria was 1 μg/ml. To assess drug binding to PBPs, the bacteria were incubated with 14C-PIP (5 μg/0.09 μCi/ml); controls, in drug-free medium.

Pilot Study: Drug Cut Autism Behavior Scores

2011 ◽  
Vol 45 (8) ◽  
pp. 21
Author(s):  
DAMIAN McNAMARA
Keyword(s):  
Pilot Study ◽  
Study Drug ◽  
Autism Behavior
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