scholarly journals Effect of H bond removal and changes in the position of the iron–sulphur head domain on the spin–lattice relaxation properties of the [2Fe–2S]2+Rieske cluster in cytochrome bc1

2015 ◽  
Vol 17 (38) ◽  
pp. 25297-25308 ◽  
Author(s):  
Marcin Sarewicz ◽  
Małgorzata Dutka ◽  
Rafał Pietras ◽  
Arkadiusz Borek ◽  
Artur Osyczka
Keyword(s):  
Membrane Protein ◽  
Electron Spin ◽  
Protein Complex ◽  
Lattice Relaxation ◽  
Spin Lattice Relaxation ◽  
Membrane Protein Complex ◽  
Relaxation Properties ◽  
Spin Lattice ◽  
Relaxation Studies ◽  
Comparative Electron

Here, comparative electron spin–lattice relaxation studies of the 2Fe–2S iron–sulphur (Fe–S) cluster embedded in a large membrane protein complex – cytochromebc1– are reported.

Pulse Shaped Dynamic Nuclear Polarization Under Magic Angle Spinning

2019 ◽  
Author(s):  
ASIF EQUBAL ◽  
Kan Tagami ◽  
Songi Han
Keyword(s):  
Electron Spin ◽  
Lattice Relaxation ◽  
Magic Angle Spinning ◽  
Spin Lattice Relaxation ◽  
Magic Angle ◽  
Total Electron ◽  
Spin Lattice ◽  
Maximum Benefit ◽  
Selective Saturation ◽  
Angle Spinning

In this paper, we report on an entirely novel way of improving the MAS-DNP efficiency by shaped μw pulse train irradiation for fast and broad-banded (FAB) saturation of the electron spin resonance. FAB-DNP achieved with Arbitrary Wave Generated shaped μw pulse trains facilitates effective and selective saturation of a defined fraction of the total electron spins, and provides superior control over the DNP efficiency under MAS. Experimental and quantum-mechanics based numerically simulated results together demonstrate that FAB-DNP significantly outperforms CW-DNP when the EPR-line of PAs is broadened by conformational distribution and exchange coupling. We demonstrate that the maximum benefit of FAB DNP is achieved when the electron spin-lattice relaxation is fast relative to the MAS frequency, i.e. at higher temperatures and/or when employing metals as PAs. Calculations predict that under short T<sub>1e </sub>conditions AWG-DNP can achieve as much as ~4-fold greater enhancement compared to CW-DNP.

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