scholarly journals Selenomethionine, p-cyanophenylalanine pairs provide a convenient, sensitive, non-perturbing fluorescent probe of local helical structure

2016 ◽  
Vol 52 (10) ◽  
pp. 2055-2058 ◽  
Author(s):  
Ivan Peran ◽  
Matthew D. Watson ◽  
Osman Bilsel ◽  
Daniel P. Raleigh
Keyword(s):  
Fluorescent Probe ◽  
Short Range ◽  
Helical Structure ◽  
Specific Probe ◽  
Site Specific ◽  
A Site

Selenomethionine is a short range quencher of p-cyanophenylalanine fluorescence and these residues provide a site-specific probe of protein helical structure.

Using a Genetically Encoded Fluorescent Amino Acid as a Site-Specific Probe to Detect Binding of Low-Molecular-Weight Compounds

2011 ◽  
Vol 9 (1) ◽  
pp. 50-57 ◽  
Author(s):  
Isaac N. Ugwumba ◽  
Kiyoshi Ozawa ◽  
Laura de la Cruz ◽  
Zhi-Qiang Xu ◽  
Anthony J. Herlt ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Amino Acid ◽  
Low Molecular Weight ◽  
Specific Probe ◽  
Site Specific ◽  
Low Molecular Weight Compounds ◽  
Fluorescent Amino Acid ◽  
A Site

Oxygen as a Site Specific Probe of the Structure of Water and Oxide Materials

2011 ◽  
Vol 107 (14) ◽  
Author(s):  
Anita Zeidler ◽  
Philip S. Salmon ◽  
Henry E. Fischer ◽  
Jörg C. Neuefeind ◽  
J. Mike Simonson ◽  
...  
Keyword(s):  
Oxide Materials ◽  
Specific Probe ◽  
Site Specific ◽  
Structure Of Water ◽  
A Site

scholarly journals Short-range homing in a site-specific fish: search and directed movements

2012 ◽  
Vol 215 (16) ◽  
pp. 2751-2759 ◽  
Author(s):  
H. Mitamura ◽  
K. Uchida ◽  
Y. Miyamoto ◽  
T. Kakihara ◽  
A. Miyagi ◽  
...  
Keyword(s):  
Short Range ◽  
Site Specific ◽  
A Site

scholarly journals Functional expression of a yeast mitochondrial intron-encoded protein requires RNA processing at a conserved dodecamer sequence at the 3' end of the gene.

1989 ◽  
Vol 9 (4) ◽  
pp. 1507-1512 ◽  
Author(s):  
H Zhu ◽  
H Conrad-Webb ◽  
X S Liao ◽  
P S Perlman ◽  
R A Butow
Keyword(s):  
Genetic Analysis ◽  
Rna Processing ◽  
Fold Increase ◽  
Functional Expression ◽  
Rrna Gene ◽  
Yeast Mitochondria ◽  
Wild Type ◽  
Site Specific ◽  
Var1 Gene ◽  
A Site

All mRNAs of yeast mitochondria are processed at their 3' ends within a conserved dodecamer sequence, 5'-AAUAAUAUUCUU-3'. A dominant nuclear suppressor, SUV3-I, was previously isolated because it suppresses a dodecamer deletion at the 3' end of the var1 gene. We have tested the effects of SUV3-1 on a mutant containing two adjacent transversions within a dodecamer at the 3' end of fit1, a gene located within the 1,143-base-pair intron of the 21S rRNA gene, whose product is a site-specific endonuclease required in crosses for the quantitative transmission of that intron to 21S alleles that lack it. The fit1 dodecamer mutations blocked both intron transmission and dodecamer cleavage, neither of which was suppressed by SUV3-1 when present in heterozygous or homozygous configurations. Unexpectedly, we found that SUV3-1 completely blocked cleavage of the wild-type fit1 dodecamer and, in SUV3-1 homozygous crosses, intron conversion. In addition, SUV3-1 resulted in at least a 40-fold increase in the amount of excised intron accumulated. Genetic analysis showed that these phenotypes resulted from the same mutation. We conclude that cleavage of a wild-type dodecamer sequence at the 3' end of the fit1 gene is essential for fit1 expression.

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