scholarly journals Near-IR mediated intracellular uncaging of NO from cell targeted hollow gold nanoparticles

2015 ◽  
Vol 51 (100) ◽  
pp. 17692-17695 ◽  
Author(s):  
Elizabeth S. Levy ◽  
Demosthenes P. Morales ◽  
John V. Garcia ◽  
Norbert O. Reich ◽  
Peter C. Ford
Keyword(s):  
Prostate Cancer ◽  
Gold Nanoparticles ◽  
Gold Nanoshells ◽  
Prostate Cancer Cells ◽  
Nir Light ◽  
Near Ir ◽  
Hollow Gold Nanoshells ◽  
Hollow Gold Nanoparticles ◽  
Spatio Temporal ◽  
Surface Modified

NIR light triggers NO delivery with unprecedented spatio-temporal control inside prostate cancer cells from surface-modified hollow gold nanoshells.

scholarly journals Engineering A11 Minibody-Conjugated, Polypeptide-Based Gold Nanoshells for Prostate Stem Cell Antigen (PSCA)–Targeted Photothermal Therapy

2016 ◽  
Vol 22 (1) ◽  
pp. 26-35 ◽  
Author(s):  
Kristine M. Mayle ◽  
Kathryn R. Dern ◽  
Vincent K. Wong ◽  
Kevin Y. Chen ◽  
Shijun Sung ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Stem Cell ◽  
Cancer Cells ◽  
Photothermal Therapy ◽  
Metastatic Prostate Cancer ◽  
Gold Nanoshells ◽  
Infrared Light ◽  
Prostate Stem Cell Antigen ◽  
Prostate Cancer Cells ◽  
Cell Antigen

Currently, there is no curative treatment for advanced metastatic prostate cancer, and options, such as chemotherapy, are often nonspecific, harming healthy cells and resulting in severe side effects. Attaching targeting ligands to agents used in anticancer therapies has been shown to improve efficacy and reduce nonspecific toxicity. Furthermore, the use of triggered therapies can enable spatial and temporal control over the treatment. Here, we combined an engineered prostate cancer–specific targeting ligand, the A11 minibody, with a novel photothermal therapy agent, polypeptide-based gold nanoshells, which generate heat in response to near-infrared light. We show that the A11 minibody strongly binds to the prostate stem cell antigen that is overexpressed on the surface of metastatic prostate cancer cells. Compared to nonconjugated gold nanoshells, our A11 minibody-conjugated gold nanoshell exhibited significant laser-induced, localized killing of prostate cancer cells in vitro. In addition, we improved upon a comprehensive heat transfer mathematical model that was previously developed by our laboratory. By relaxing some of the assumptions of our earlier model, we were able to generate more accurate predictions for this particular study. Our experimental and theoretical results demonstrate the potential of our novel minibody-conjugated gold nanoshells for metastatic prostate cancer therapy.

Multifunctional targeted therapy system based on99mTc/177Lu-labeled gold nanoparticles-Tat(49-57)-Lys3-bombesin internalized in nuclei of prostate cancer cells

2013 ◽  
Vol 56 (13) ◽  
pp. 663-671 ◽  
Author(s):  
Nallely Jiménez-Mancilla ◽  
Guillermina Ferro-Flores ◽  
Clara Santos-Cuevas ◽  
Blanca Ocampo-García ◽  
Myrna Luna-Gutiérrez ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Gold Nanoparticles ◽  
Targeted Therapy ◽  
Cancer Cells ◽  
Prostate Cancer Cells

scholarly journals Enhanced radiation sensitivity in prostate cancer by gold-nanoparticles

2008 ◽  
Vol 31 (3) ◽  
pp. 160 ◽  
Author(s):  
Xiaojing Zhang ◽  
James Z. Xing ◽  
Jie Chen ◽  
Lawrence Ko ◽  
John Amanie ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Gold Nanoparticles ◽  
Growth Inhibition ◽  
Cancer Cells ◽  
Radiation Sensitivity ◽  
Human Prostate ◽  
In Vivo Studies ◽  
Prostate Cancer Cells ◽  
Nanoparticle Uptake

Purpose: Nanotechnology is an emerging field with significant translational potential in medicine. In this study, we applied gold nanoparticles (GNP) to enhance radiation sensitivity and growth inhibition in radiation-resistant human prostate cancer cells. Methods: Gold nanoparticles (GNPs) were synthesized using HAuCl4 as the gold particle source and NaBH4 as the reductant. Either thio-glucose or sodium citrate was then added to the solution separately to bind the GNPs to form thio-glucose-capped gold nanoparticles (Glu-GNP) and neutral gold nanoparticles (TGS-GNPs). Human prostate carcinoma DU-145 cells were exposed to vehicle, irradiation, 15nM TGS-GNPs, or 15nM Glu-GNPs, or GNPs plus irradiation. The uptake assays of GNP were performed using hemocytometer to count cells and the mass spectrometry was applied to calculate gold mass. The cytotoxicity induced by GNPs, irradiation, or GNPs plus irradiation was measured using a standard colorimetric MTT assay. Results: Exposure to Glu-GNPs resulted in a three times increase of nanoparticle uptake compared to that of TGS-GNPs in each target cell (p < 0.005). Cytoplasmic intracellular uptake of both TGS-GNPs and Glu-GNPs resulted in a growth inhibition by 30.57% and 45.97% respectively, comparing to 15.88% induced by irradiation alone, in prostate cancer cells after exposure to the irradiation. Glu-GNPs showed a greater enhancement, 1.5 to 2 fold increases within 72 hours, on irradiation cytotoxicity compared to TGS-GNPs. Tumour killing, however, did not appear to correlate linearly with nanoparticle uptake concentrations. Conclusion: These results showed that functional glucose-bound gold nanoparticles enhanced radiation sensitivity and toxicity in prostate cancer cells. In vivo studies will be followed to verify our research findings.

scholarly journals Targeting prostate cancer cells with PSMA inhibitor-guided gold nanoparticles

2013 ◽  
Vol 23 (2) ◽  
pp. 565-568 ◽  
Author(s):  
Benjamin B. Kasten ◽  
Tiancheng Liu ◽  
Jessie R. Nedrow-Byers ◽  
Paul D. Benny ◽  
Clifford E. Berkman
Keyword(s):  
Prostate Cancer ◽  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Prostate Cancer Cells ◽  
Psma Inhibitor

Efficacy of Laser-Activated Gold Nanoshells in Ablating Prostate Cancer Cells in Vitro

2007 ◽  
Vol 21 (8) ◽  
pp. 939-943 ◽  
Author(s):  
Joshua M. Stern ◽  
Jennifer Stanfield ◽  
Yair Lotan ◽  
Sangtae Park ◽  
Jer-Tsong Hsieh ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Gold Nanoshells ◽  
Prostate Cancer Cells
Sign in / Sign up

Export Citation Format

Share Document