Elucidating the relationship between superoxide anion levels and lifespan using an enhanced two-photon fluorescence imaging probe

2015 ◽  
Vol 51 (47) ◽  
pp. 9710-9713 ◽  
Author(s):  
Wen Zhang ◽  
Xin Wang ◽  
Ping Li ◽  
Fang Huang ◽  
Hui Wang ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Cross Section ◽  
Absorption Cross Section ◽  
Superoxide Anion ◽  
Absorption Cross ◽  
Fluorescence Response ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
The Relationship ◽  
Photon Fluorescence

We report a new reversible fluorescent two-photon (TP) probe (PY-CA) with high TP absorption cross section and pH-independent fluorescence response, which allow monitoring of O2˙− fluxes dynamically, selectively and sensitively.

scholarly journals Engineering a FRET strategy to achieve a ratiometric two-photon fluorescence response with a large emission shift and its application to fluorescence imaging

2015 ◽  
Vol 6 (4) ◽  
pp. 2360-2365 ◽  
Author(s):  
Lin Yuan ◽  
Fangping Jin ◽  
Zebing Zeng ◽  
Chengbin Liu ◽  
Shenglian Luo ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Fluorescence Response ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence ◽  
In Cells

A FRET strategy was applied to develop a ratiometric two-photon fluorescent probe with a large emission shift for imaging in cells and tissues.

scholarly journals Two-photon fluorescence imaging reveals a Golgi apparatus superoxide anion-mediated hepatic ischaemia-reperfusion signalling pathway

2019 ◽  
Vol 10 (3) ◽  
pp. 879-883 ◽  
Author(s):  
Wen Zhang ◽  
Jiao Zhang ◽  
Ping Li ◽  
Jihong Liu ◽  
Di Su ◽  
...  
Keyword(s):  
Golgi Apparatus ◽  
Fluorescence Imaging ◽  
Superoxide Anion ◽  
Fluorescence Probe ◽  
Signalling Pathway ◽  
Two Photon ◽  
Ischaemia Reperfusion ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

We developed a two-photon fluorescence probe for the dynamic and reversible detection of O2˙− levels in the Golgi apparatus.

PEGYLATED CONJUGATED OLIGOMERS FOR TARGETED TWO-PHOTON FLUORESCENCE IMAGING OF CANCER CELLS

2013 ◽  
Vol 01 (03) ◽  
pp. 1340011
Author(s):  
GUAN WANG ◽  
JUNLONG GENG ◽  
XINHAI ZHANG ◽  
GUICHUAN XING ◽  
TZE CHIEN SUM ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Fluorescence Imaging ◽  
Cross Section ◽  
Fluorescence Quantum Yield ◽  
Photon Absorption ◽  
Fluorescence Measurement ◽  
Experimental Conditions ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

A water-dispersible PEGylated conjugated oligomer (PEGTVFVBN) is synthesized for two-photon fluorescence imaging applications. PEGTVFVBN could aggregate into nanoparticles of ~ 110 nm size in H 2 O , which show a fluorescence quantum yield of 0.12, 20 times higher than that for its cationic counterpart (TVFVBC). Two-photon fluorescence measurement reveals a maximum two-photon absorption (TPA) cross-section of 263 GM at 780 nm based on molecules for PEGTVFVBN nanoparticles in H 2 O , while the TPA spectra of TVFVBC are barely detectable under the same experimental conditions. The N , N' -diacetylchitobiotic acid conjugated derivative (ChPEGTVFVBN) could also aggregate into nanoparticles of ~ 90 nm sizes, showing a slightly smaller fluorescence quantum yield of 0.08 and a maximum TPA cross-section of 213 GM at 780 nm. ChPEGTVFVBN could be specifically internalized by vimentin over expressing Hela cells through a receptor mediated endocytosis, giving two-photon fluorescence images in a bright and high contrast manner. In addition, both PEGTVFVBN and ChPEGTVFVBN show low cytotoxicity, which is essential for cell imaging applications. This study thus highlights the molecular engineering strategy of PEGylation to construct robust TPA materials for two-photon fluorescence imaging applications.

Lysosomal targeted NIR photosensitizer for photodynamic therapy and two-photon fluorescent imaging

2021 ◽  
Author(s):  
Ruiyuan Liu ◽  
Yuping Zhou ◽  
Di Zhang ◽  
Genghan He ◽  
Chuang Liu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Fluorescent Imaging ◽  
High Fidelity ◽  
Design And Synthesis ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Design and synthesis of near-infrared (NIR) emissive fluorophore for imaging of organelle and photodynamic therapy has received enormous attention. Hence, NIR emissive fluorophore of high-fidelity lysosome targeting, two-photon fluorescence imaging,...

Enhanced Two-Photon Fluorescence Imaging and Therapy of Cancer Cells via Gold@Bridged Silsesquioxane Nanoparticles

Small ◽  
2014 ◽  
Vol 11 (3) ◽  
pp. 295-299 ◽  
Author(s):  
Jonas Croissant ◽  
Marie Maynadier ◽  
Olivier Mongin ◽  
Vincent Hugues ◽  
Mireille Blanchard-Desce ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Two Photon ◽  
Bridged Silsesquioxane ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence
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