scholarly journals Combination of automated solid-phase and enzymatic oligosaccharide synthesis provides access to α(2,3)-sialylated glycans

2015 ◽  
Vol 51 (28) ◽  
pp. 6183-6185 ◽  
Author(s):  
Richard J. Fair ◽  
Heung Sik Hahm ◽  
Peter H. Seeberger
Keyword(s):  
Chemical Synthesis ◽  
Solid Phase ◽  
Oligosaccharide Synthesis ◽  
Synthetic Strategy ◽  
Enzymatic Sialylation ◽  
Phase Chemical

A synthetic strategy combining automated solid-phase chemical synthesis and enzymatic sialylation was developed to access α(2,3)-sialylated glycans.

Efficient Solid-Phase Chemical Synthesis of 5′-Triphosphates of DNA, RNA, and their Analogues

2010 ◽  
Vol 12 (10) ◽  
pp. 2190-2193 ◽  
Author(s):  
Ivan Zlatev ◽  
Thomas Lavergne ◽  
Françoise Debart ◽  
Jean-Jacques Vasseur ◽  
Muthiah Manoharan ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Solid Phase ◽  
Phase Chemical

Solution and solid phase chemical synthesis of arabinonucleotides

1989 ◽  
Vol 67 (5) ◽  
pp. 831-839 ◽  
Author(s):  
Masad José Damha ◽  
Nassim Usman ◽  
Kelvin Kenneth Ogilvie
Keyword(s):  
Chemical Synthesis ◽  
Solid Phase ◽  
Building Blocks ◽  
Convenient Procedure ◽  
Phase Chemical

A fast and convenient procedure for the chemical synthesis of arabinonucleotides, which eliminates the multistep protection of the arabinonucleoside building blocks, is described. The results of these studies were successfully applied to the automated chemical synthesis of the hexanucleotide 5′-aUpaApaUpaApaUpaA-3′. Both solution and solid phase phosphite triester procedures are described. Keywords: arabinonucleotides, arabinophosphoramidites, automated chemical synthesis, protected arabinonucleosides.

Fmoc-based solid phase chemical synthesis of 71-meric neuregulin 1-β1, an epidermal growth factor-like domain

2008 ◽  
Vol 14 (3) ◽  
pp. 261-266
Author(s):  
Taeko Kakizawa ◽  
Shizuyo Koide-Yoshida ◽  
Tooru Kimura ◽  
Hiromasa Uchimura ◽  
Yoshio Hayashi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Chemical Synthesis ◽  
Solid Phase ◽  
Neuregulin 1 ◽  
Epidermal Growth ◽  
Phase Chemical

Solid-Phase Chemical Synthesis of Phosphonoacetate and Thiophosphonoacetate Oligodeoxynucleotides

2003 ◽  
Vol 125 (4) ◽  
pp. 940-950 ◽  
Author(s):  
Douglas J. Dellinger ◽  
David M. Sheehan ◽  
Nanna K. Christensen ◽  
James G. Lindberg ◽  
Marvin H. Caruthers
Keyword(s):  
Chemical Synthesis ◽  
Solid Phase ◽  
Phase Chemical

A NOVEL APPROACH TO SOLID PHASE CHEMICAL SYNTHESIS OF OLIGONUCLEOTIDE mRNA CAP ANALOGS

2005 ◽  
Vol 24 (5-7) ◽  
pp. 601-605 ◽  
Author(s):  
Jacek Jemielity ◽  
Petri Heinonen ◽  
Harri Lönnberg ◽  
Edward Darzynkiewicz
Keyword(s):  
Chemical Synthesis ◽  
Solid Phase ◽  
Novel Approach ◽  
Phase Chemical

scholarly journals Solid-Phase Chemical Synthesis of Stable Isotope-Labeled RNA to Aid Structure and Dynamics Studies by NMR Spectroscopy

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3476 ◽  
Author(s):  
Owen Becette ◽  
Lukasz T. Olenginski ◽  
Theodore K. Dayie
Keyword(s):  
Nmr Spectroscopy ◽  
Stable Isotope ◽  
Chemical Synthesis ◽  
Rna Structure ◽  
Solid Phase ◽  
Rna World ◽  
Dynamic Studies ◽  
Labeled Rna ◽  
Spectroscopy Studies ◽  
Phase Chemical

RNA structure and dynamic studies by NMR spectroscopy suffer from chemical shift overlap and line broadening, both of which become worse as RNA size increases. Incorporation of stable isotope labels into RNA has provided several solutions to these limitations. Nevertheless, the only method to circumvent the problem of spectral overlap completely is the solid-phase chemical synthesis of RNA with labeled RNA phosphoramidites. In this review, we summarize the practical aspects of this methodology for NMR spectroscopy studies of RNA. These types of investigations lie at the intersection of chemistry and biophysics and highlight the need for collaborative efforts to tackle the integrative structural biology problems that exist in the RNA world. Finally, examples of RNA structure and dynamic studies using labeled phosphoramidites are highlighted.

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