A sensitive whole-cell biosensor for the simultaneous detection of a broad-spectrum of toxic heavy metal ions

2015 ◽  
Vol 51 (27) ◽  
pp. 5917-5920 ◽  
Author(s):  
S. Cerminati ◽  
F. C. Soncini ◽  
S. K. Checa
Keyword(s):  
Heavy Metal Ions ◽  
Simultaneous Detection ◽  
High Sensitivity ◽  
Toxic Heavy Metal ◽  
Whole Cell ◽  
Fluorescent Biosensors ◽  
Lead And Cadmium ◽  
Whole Cell Biosensor ◽  
Bioavailable Mercury ◽  
Design Construction

Design, construction and validation of the first whole-cell fluorescent biosensors reporting, simultaneously and with high sensitivity, bioavailable mercury, lead and cadmium in water samples.

scholarly journals Detection of Bioavailable Cadmium by Double-Color Fluorescence Based on a Dual-Sensing Bioreporter System

2021 ◽  
Vol 12 ◽  
Author(s):  
Chang-ye Hui ◽  
Yan Guo ◽  
Jian Wu ◽  
Lisa Liu ◽  
Xue-qin Yang ◽  
...  
Keyword(s):  
Anthropogenic Activities ◽  
Simultaneous Detection ◽  
Environmental Water ◽  
High Concentration ◽  
Whole Cell ◽  
Specificity And Sensitivity ◽  
Improved Performance ◽  
Whole Cell Biosensor ◽  
Cell Biosensor ◽  
Dual Sensing

Cadmium (Cd) is carcinogenic to humans and can accumulate in the liver, kidneys, and bones. There is widespread presence of cadmium in the environment as a consequence of anthropogenic activities. It is important to detect cadmium in the environment to prevent further exposure to humans. Previous whole-cell biosensor designs were focused on single-sensing constructs but have had difficulty in distinguishing cadmium from other metal ions such as lead (Pb) and mercury (Hg). We developed a dual-sensing bacterial bioreporter system to detect bioavailable cadmium by employing CadC and CadR as separate metal sensory elements and eGFP and mCherry as fluorescent reporters in one genetic construct. The capability of this dual-sensing biosensor was proved to simultaneously detect bioavailable cadmium and its toxic effects using two sets of sensing systems while still maintaining similar specificity and sensitivity of respective signal-sensing biosensors. The productions of double-color fluorescence were directly proportional to the exposure concentration of cadmium, thereby serving as an effective quantitative biosensor to detect bioavailable cadmium. This novel dual-sensing biosensor was then validated to respond to Cd(II) spiked in environmental water samples. This is the first report of the development of a novel dual-sensing, whole-cell biosensor for simultaneous detection of bioavailable cadmium. The application of two biosensing modules provides versatile biosensing signals and improved performance that can make a significant impact on monitoring high concentration of bioavailable Cd(II) in environmental water to reduce human exposure to the harmful effects of cadmium.

scholarly journals Rapid Screening of Quorum-Sensing Signal N-Acyl Homoserine Lactones by an In Vitro Cell-Free Assay

2008 ◽  
Vol 74 (12) ◽  
pp. 3667-3671 ◽  
Author(s):  
Tomohiro Kawaguchi ◽  
Yung Pin Chen ◽  
R. Sean Norman ◽  
Alan W. Decho
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Quorum Sensing ◽  
High Sensitivity ◽  
Homoserine Lactone ◽  
Assay System ◽  
Rapid Screening ◽  
Whole Cell ◽  
Bacterial Quorum ◽  
Whole Cell Biosensor

ABSTRACT A simple, sensitive, and rapid cell-free assay system was developed for detection of N-acyl homoserine lactone (AHL) autoinducers involved in bacterial quorum sensing (QS). The present approach improves upon previous whole-cell biosensor-based approaches in its utilization of a cell-free assay approach to conduct bioassays. The cell-free assay was derived from the AHL biosensor bacterium Agrobacterium tumefaciens NTL4(pCF218)(pCF372), allowing the expression of β-galactosidase upon addition of exogenous AHLs. We have shown that β-galactosidase expression is possible in cell-free solution [lysate from Agrobacterium tumefaciens NTL4(pCF218)(pCF372) culture]. Assay detection limits with the use of chromogenic substrate X-Gal (5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside) ranged from approximately 100 nM to 300 nM depending on the specific AHL. Replacement (of X-Gal) with the luminescent substrate Beta-Glo increased sensitivity to AHLs by 10-fold. A major advantage of the cell-free assay system is elimination of time-consuming steps for biosensor cell culture conditioning, which are required prior to whole-cell bioassays. This significantly reduced assay times from greater than 24 h to less than 3 h, while maintaining high sensitivity. Assay lysate may be prepared in bulk and stored (−80°C) over 6 months for future use. Finally, the present protocol may be adapted for use with other biosensor strains and be used in high-throughput AHL screening of bacteria or metagenomic libraries.

scholarly journals Sensitive electrochemical sensor based on poly(l-glutamic acid)/graphene oxide composite material for simultaneous detection of heavy metal ions

RSC Advances ◽  
2019 ◽  
Vol 9 (30) ◽  
pp. 17325-17334 ◽  
Author(s):  
Wei Yi ◽  
Zihua He ◽  
Junjie Fei ◽  
Xiaohua He
Keyword(s):  
Heavy Metal ◽  
Composite Material ◽  
Metal Ions ◽  
Electrochemical Sensor ◽  
Heavy Metal Ions ◽  
High Stability ◽  
Simultaneous Detection ◽  
High Sensitivity ◽  
Good Reproducibility ◽  
Oxide Composite Material

A novel electrochemical sensor with high stability and good reproducibility for the simultaneous detection of heavy metal ions was prepared by using PGA/GO to modify the GCE, showing high sensitivity of superior to most of the reported values.

A highly-sensitive electrochemical sensor for the simultaneous detection of Cd2+ and Pb2+ using liquid phase-exfoliated graphene

2014 ◽  
Vol 6 (15) ◽  
pp. 5760-5765 ◽  
Author(s):  
Guishen Liu ◽  
Jianpeng Chen ◽  
Xiaodong Hou ◽  
Wensheng Huang
Keyword(s):  
Heavy Metal ◽  
Liquid Phase ◽  
Metal Ions ◽  
Electrochemical Sensor ◽  
Analytical Methods ◽  
Heavy Metal Ions ◽  
Simultaneous Detection ◽  
Toxic Heavy Metal ◽  
Exfoliated Graphene ◽  
Highly Sensitive

It is quite important to develop sensitive and simple analytical methods for the detection of toxic heavy metal ions, such as Cd2+ and Pb2+.

scholarly journals Novel nanoscale Yb-MOF used as highly efficient electrode for simultaneous detection of heavy metal ions

2021 ◽  
Vol 56 (13) ◽  
pp. 8172-8185
Author(s):  
Manh B. Nguyen ◽  
Dau Thi Ngoc Nga ◽  
Vu Thi Thu ◽  
Benoît Piro ◽  
Thuan Nguyen Pham Truong ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Heavy Metal Ions ◽  
Simultaneous Detection ◽  
Highly Efficient

scholarly journals A Short Review on Recent Advances of Hydrogel-Based Adsorbents for Heavy Metal Ions

Metals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 864
Author(s):  
Suguna Perumal ◽  
Raji Atchudan ◽  
Thomas Nesakumar Jebakumar Immanuel Edison ◽  
Rajendran Suresh Babu ◽  
Petchimuthu Karpagavinayagam ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Heavy Metal Ions ◽  
Large Scale ◽  
Water Bodies ◽  
Dimensional Structure ◽  
High Porosity ◽  
Adsorption Method ◽  
Toxic Heavy Metal ◽  
Practical Applications

The growth of industry fulfills our necessity and promotes economic development. However, pollutants from such industries pollute water bodies which pose a high risk for living organisms. Thus, researchers have been urged to develop an efficient method to remove toxic heavy metal ions from water bodies. The adsorption method shows promising results for the removal of heavy metal ions and is easy to operate on a large scale, thus can be applied to practical applications. Numerous adsorbents were developed and reported, among them hydrogels, which attract great attention because of the reusability, ease of preparation, and handling. Hydrogels are generally prepared by the cross-linking of polymers that result in a three-dimensional structure, showing high porosity and high functionality. They are hydrophilic in nature because of the functional groups, and are non-toxic. Thus, this review provides various methods of hydrogel adsorbents preparation and summarizes recent progress in the use of hydrogel adsorbents for the removal of heavy metal ions. Further, the mechanism involved in the removal of heavy metal ions is briefly discussed. The most recent studies about the adsorption method for the treatment of heavy metal ions contaminated water are presented.

scholarly journals Microfluidics-Based Plasmonic Biosensing System Based on Patterned Plasmonic Nanostructure Arrays

Micromachines ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 826
Author(s):  
Yanting Liu ◽  
Xuming Zhang
Keyword(s):  
Plasmon Resonance ◽  
Point Of Care ◽  
Simultaneous Detection ◽  
High Sensitivity ◽  
Microfluidic Chips ◽  
Label Free ◽  
Plasmonic Nanostructure ◽  
Point Of Care Diagnostics ◽  
Surface Enhanced Raman ◽  
Nanostructure Arrays

This review aims to summarize the recent advances and progress of plasmonic biosensors based on patterned plasmonic nanostructure arrays that are integrated with microfluidic chips for various biomedical detection applications. The plasmonic biosensors have made rapid progress in miniaturization sensors with greatly enhanced performance through the continuous advances in plasmon resonance techniques such as surface plasmon resonance (SPR) and localized SPR (LSPR)-based refractive index sensing, SPR imaging (SPRi), and surface-enhanced Raman scattering (SERS). Meanwhile, microfluidic integration promotes multiplexing opportunities for the plasmonic biosensors in the simultaneous detection of multiple analytes. Particularly, different types of microfluidic-integrated plasmonic biosensor systems based on versatile patterned plasmonic nanostructured arrays were reviewed comprehensively, including their methods and relevant typical works. The microfluidics-based plasmonic biosensors provide a high-throughput platform for the biochemical molecular analysis with the advantages such as ultra-high sensitivity, label-free, and real time performance; thus, they continue to benefit the existing and emerging applications of biomedical studies, chemical analyses, and point-of-care diagnostics.

scholarly journals Influence of the luxR Regulatory Gene Dosage and Expression Level on the Sensitivity of the Whole-Cell Biosensor to Acyl-Homoserine Lactone

Biosensors ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 166
Author(s):  
Sergey Bazhenov ◽  
Uliana Novoyatlova ◽  
Ekaterina Scheglova ◽  
Vadim Fomin ◽  
Svetlana Khrulnova ◽  
...  
Keyword(s):  
Gene Dosage ◽  
Regulatory Gene ◽  
Homoserine Lactone ◽  
Threshold Concentration ◽  
Expression Level ◽  
Whole Cell ◽  
Lux Biosensors ◽  
Order Of Magnitude ◽  
Whole Cell Biosensor ◽  
Cell Biosensor

Aliivibrio fischeri LuxR and Aliivibrio logei LuxR1 and LuxR2 regulatory proteins are quorum sensing transcriptional (QS) activators, inducing promoters of luxICDABEG genes in the presence of an autoinducer (3-oxo-hexanoyl-l-homoserine lactone). In the Aliivibrio cells, luxR genes are regulated by HNS, CRP, LitR, etc. Here we investigated the role of the luxR expression level in LuxI/R QS system functionality and improved the whole-cell biosensor for autoinducer detection. Escherichia coli-based bacterial lux-biosensors were used, in which Photorhabdus luminescensluxCDABE genes were controlled by LuxR-dependent promoters and luxR, luxR1, or luxR2 regulatory genes. We varied either the dosage of the regulatory gene in the cells using additional plasmids, or the level of the regulatory gene expression using the lactose operon promoter. It was shown that an increase in expression level, as well as dosage of the regulatory gene in biosensor cells, leads to an increase in sensitivity (the threshold concentration of AI is reduced by one order of magnitude) and to a two to threefold reduction in response time. The best parameters were obtained for a biosensor with an increased dosage of luxRA. fischeri (sensitivity to 3-oxo-hexanoyl-l-homoserine lactone reached 30–100 pM).

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