Split aptamer mediated endonuclease amplification for small-molecule detection

2015 ◽  
Vol 51 (20) ◽  
pp. 4196-4199 ◽  
Author(s):  
Qing Li ◽  
Yan-Dan Wang ◽  
Guo-Li Shen ◽  
Hao Tang ◽  
Ru-Qin Yu ◽  
...  
Keyword(s):  
Small Molecule ◽  
Highly Sensitive ◽  
Small Molecule Detection ◽  
Amplification Strategy

A novel, highly sensitive split aptamer mediated endonuclease amplification strategy for the construction of aptameric sensors is reported.

scholarly journals High-sensitivity small-molecule detection of microcystin-LR cyano-toxin using a terahertz-aptamer biosensor

The Analyst ◽  
2021 ◽  
Author(s):  
Ahmed Mohamed ◽  
Ryan Walsh ◽  
Mohamed Cherif ◽  
Hassan A. Hafez ◽  
Xavier Ropagnol ◽  
...  
Keyword(s):  
Small Molecule ◽  
High Sensitivity ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Small Molecule Detection

We demonstrate the rapid and highly sensitive detection of a small molecule, microcystin-LR (MC-LR) toxin using an aptasensor based on a terahertz (THz) emission technique named the terahertz chemical microscope (TCM).

A highly sensitive and widely adaptable plasmonic aptasensor using berberine for small-molecule detection

2017 ◽  
Vol 97 ◽  
pp. 292-298 ◽  
Author(s):  
Jin-Ho Park ◽  
Ju-Young Byun ◽  
Hyungjun Jang ◽  
Donggu Hong ◽  
Min-Gon Kim
Keyword(s):  
Small Molecule ◽  
Highly Sensitive ◽  
Small Molecule Detection

Binding affinity-guided design of a highly sensitive noncompetitive immunoassay for small molecule detection

2021 ◽  
Vol 351 ◽  
pp. 129270
Author(s):  
Yuchen Bai ◽  
Yahui Wang ◽  
Qiang Li ◽  
Leina Dou ◽  
Minggang Liu ◽  
...  
Keyword(s):  
Binding Affinity ◽  
Small Molecule ◽  
Highly Sensitive ◽  
Small Molecule Detection

A highly sensitive electrochemical microRNA-21 biosensor based on intercalating methylene blue signal amplification and a highly dispersed gold nanoparticles/graphene/polypyrrole composite

The Analyst ◽  
2021 ◽  
Vol 146 (8) ◽  
pp. 2679-2688
Author(s):  
Chammari Pothipor ◽  
Noppadol Aroonyadet ◽  
Suwussa Bamrungsap ◽  
Jaroon Jakmunee ◽  
Kontad Ounnunkad
Keyword(s):  
Methylene Blue ◽  
Gold Nanoparticles ◽  
Signal Amplification ◽  
Electrochemical Biosensor ◽  
Highly Sensitive ◽  
Highly Dispersed ◽  
Polypyrrole Composite ◽  
Amplification Strategy ◽  
Dispersed Gold ◽  
Potential Tool

An ultrasensitive electrochemical biosensor based on a gold nanoparticles/graphene/polypyrrole composite modified electrode and a signal amplification strategy employing methylene blue is developed as a potential tool for the detection of miRNA-21.

Target-responsive dumbbell probe-mediated rolling circle amplification strategy for highly sensitive Hg2+ detection

RSC Advances ◽  
2014 ◽  
Vol 4 (51) ◽  
pp. 27091-27097 ◽  
Author(s):  
Qingwang Xue ◽  
Yanqin Lv ◽  
Yuanfu Zhang ◽  
Shuling Xu ◽  
Qiaoli Yue ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Selective Detection ◽  
Label Free ◽  
Rolling Circle ◽  
Fluorescent Sensing ◽  
Highly Sensitive ◽  
Mercuric Ions ◽  
Amplification Strategy

A novel label-free amplified fluorescent sensing scheme based on target-responsive dumbbell probe-mediated rolling circle amplification (D-RCA) has been developed for sensitive and selective detection of mercuric ions.

scholarly journals A Simple Displacement Aptamer Assay on Resistive Pulse Sensor for Small Molecule Detection

2020 ◽  
Author(s):  
Rhushabh Maugi ◽  
bernadette gamble ◽  
david bunka ◽  
Mark Platt
Keyword(s):  
Small Molecules ◽  
Small Molecule ◽  
Label Free ◽  
Resistive Pulse ◽  
Pulse Sensor ◽  
Label Free Detection ◽  
Ssdna Aptamer ◽  
Aptamer Assay ◽  
Small Molecule Detection ◽  
Surface Changes

A universal aptamer-based sensing strategy is proposed using DNA modified nanocarriers and Resistive Pulse Sensing for the rapid and label free detection of small molecules. The surface of a magnetic nanocarrier was first modified with a ssDNA aka linker which is designed to be partially complimentary in sequence to a ssDNA aptamer. The aptamer and linker form a stable dsDNA complex on the nanocarriers surface. Upon the addition of the target molecule, a conformational change takes place where the aptamer preferentially binds to the target over the linker; causing the aptamer to be released into solution. The RPS measures the change in velocity of the nanocarrier as its surface changes from dsDNA to ssDNA, and its velocity is used as a proxy for the concentration of the target. We illustrate the versatility of the assay by demonstrating the detection of the antibiotic Moxifloxacin, and chemotherapeutics Imatinib and Irinotecan.

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