Systematic adjustment of charge densities and size of polyglycerol amines reduces cytotoxic effects and enhances cellular uptake

2015 ◽  
Vol 3 (11) ◽  
pp. 1459-1465 ◽  
Author(s):  
Markus Hellmund ◽  
Katharina Achazi ◽  
Falko Neumann ◽  
Bala N. S. Thota ◽  
Nan Ma ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Charge Density ◽  
Cellular Uptake ◽  
Systematic Study ◽  
Effective Charge ◽  
Cytotoxic Effects ◽  
Charge Densities

Excessive cationic charge density of polyplexes during cellular uptake is still a major hurdle for gene delivery. A systematic study on cytotoxic effects caused by effective charge density related to size showed moderate loaded hPG amines to be higher potential as low/high ones.

Cytotoxic Effect of 5-Fluorouracil-loaded Polymer-coated Magnetite Nanographene Oxide Combined with Radiofrequency

2018 ◽  
Vol 18 (8) ◽  
pp. 1148-1155 ◽  
Author(s):  
Leili Asadi ◽  
Sakine Shirvalilou ◽  
Sepideh Khoee ◽  
Samideh Khoei
Keyword(s):  
Cellular Uptake ◽  
Cancer Cell Line ◽  
Superparamagnetic Iron Oxide ◽  
Colon Cancer Cell Line ◽  
Cytotoxic Effects ◽  
Proliferation Capacity ◽  
Capacity Level ◽  
Colony Number ◽  
Nano Graphene ◽  
Dialysis Bag

Background: Despite the development of conventional therapies including surgery, radiotherapy, chemotherapy and hyperthermia, the prognosis remains very poor. Recently, integration of conventional therapy and multifunctional nanoparticles have attracted a lot of attention because it produces a synergistic effect and better diagnostic and therapeutic efficiency. Objective: This study aimed to investigate the uptake and cytotoxic effects of Polycaprolactone (PCL)/chitosan (CHI)-coated Superparamagnetic Iron Oxide Nano-Graphene Oxide (SPION-NGO) as a carrier of 5-fluorouracil (5-Fu) and Radiofrequency (RF) hyperthermia using an Alternate Magnetic Field (AMF) with 13.56 MHz frequency on the proliferation capacity level of CT26 colon cancer cell line in a monolayer culture. Method: The release of the newly synthesised 5-Fu-loaded PCL/CHI-SPION-NGO was measured in Phosphate Buffered Saline (PBS) using the dialysis bag method. The cellular uptake of 5-Fu-loaded PCL/CHI-SPIONNGO was measured using Atomic Absorption Spectroscopy (AAS). The cytotoxic effects of 5-Fu, 5-Fu- PCL/CHI-SPION-NGO and PCL/CHI-SPION-NGO with and without RF hyperthermia were determined using the colony formation assay. Results: Particle size and zeta potential of 5-Fu-PCL/CHI-SPION-NGO and PCL/CHI-SPION-NGO were 61.2 nm and -1.87 mV and 43.4 nm and -10.19 mV, respectively. Spectroscopy results demonstrated that the cellular uptake of 5-Fu-PCL/CHI-SPION-NGO increased with elevated nanostructure concentrations. The results revealed that the proliferation capacity of the cells decreased with 5-Fu or 5-Fu-PCL/CHI-SPION-NGO in combination with RF hyperthermia. Furthermore, extent of reduction in colony number following treatment with 5-Fu-PCL/CHI-SPION-NGO in combination with AMF was significantly more than 5-Fu + hyperthermia. Conclusion: Therefore, PCL/CHI-SPION-NGO can deliver 5-Fu more efficiently into the CT26 cells.

Boosting transfection efficiency: A systematic study using layer-by-layer based gene delivery platform

2021 ◽  
pp. 112161
Author(s):  
Yana V. Tarakanchikova ◽  
Dmitrii S. Linnik ◽  
Tatiana Mashel ◽  
Albert R. Muslimov ◽  
Sergey Pavlov ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Systematic Study ◽  
Transfection Efficiency ◽  
Layer By Layer ◽  
Delivery Platform

Penetration and preferential binding of charged nanoparticles to mixed lipid monolayers: interplay of lipid packing and charge density

Soft Matter ◽  
2020 ◽  
Author(s):  
Anurag Chaudhury ◽  
Koushik Debnath ◽  
Wei Bu ◽  
Nikhil R. Jana ◽  
Jaydeep Kumar Basu
Keyword(s):  
Charge Density ◽  
Biomedical Applications ◽  
Cell Membranes ◽  
Lipid Monolayers ◽  
Cytotoxic Effects ◽  
Lipid Packing ◽  
Preferential Binding ◽  
Charged Nanoparticles

Designing of nanoparticles (NPs) for biomedical applications or mitigating their cytotoxic effects require microscopic understanding of their interactions with cell membranes. Such insight is best obtained by studying model biomembranes...

Brushlike Cationic Polymers with Low Charge Density for Gene Delivery

2017 ◽  
Vol 19 (5) ◽  
pp. 1410-1415 ◽  
Author(s):  
Jonathan O’Keeffe Ahern ◽  
Sigen A ◽  
Dezhong Zhou ◽  
Yongsheng Gao ◽  
Jing Lyu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Charge Density ◽  
Cationic Polymers ◽  
Low Charge

Cationic long-chain hyperbranched poly(ethylene glycol)s with low charge density for gene delivery

2013 ◽  
Vol 4 (2) ◽  
pp. 393-401 ◽  
Author(s):  
Chunlai Tu ◽  
Nan Li ◽  
Lijuan Zhu ◽  
Linzhu Zhou ◽  
Yue Su ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Ethylene Glycol ◽  
Charge Density ◽  
Poly Ethylene Glycol ◽  
Hyperbranched Poly ◽  
Poly Ethylene ◽  
Low Charge ◽  
Long Chain

scholarly journals DNA–DNA Interactions in Tight Supercoils Are Described by a Small Effective Charge Density

2010 ◽  
Vol 105 (15) ◽  
Author(s):  
Christopher Maffeo ◽  
Robert Schöpflin ◽  
Hergen Brutzer ◽  
René Stehr ◽  
Aleksei Aksimentiev ◽  
...  
Keyword(s):  
Charge Density ◽  
Effective Charge ◽  
Dna Interactions

Cellular uptake and gene delivery using layered double hydroxide nanoparticles

2013 ◽  
Vol 1 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Shuangde Li ◽  
Jinghuan Li ◽  
Chengle J. Wang ◽  
Qiang Wang ◽  
M. Zameel Cader ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Layered Double Hydroxide ◽  
Cellular Uptake ◽  
Double Hydroxide ◽  
Layered Double Hydroxide Nanoparticles

Charge density studies on methylene blue – a potential anti-Alzheimer agent

2018 ◽  
Vol 73 (2) ◽  
pp. 99-108 ◽  
Author(s):  
Peter Luger ◽  
Birger Dittrich ◽  
Leonard Benecke ◽  
Hannes Sterzel
Keyword(s):  
Methylene Blue ◽  
Charge Density ◽  
Molecular Electrostatic Potential ◽  
Positive Charge ◽  
Atomic Charges ◽  
Charge Densities ◽  
Density Distributions ◽  
Atomic Properties ◽  
Counter Ions ◽  
Medical Interest

AbstractMotivated by the medical interest in methylene blue as potential anti-Alzheimer agent, the charge densities of three salt structures containing the methylene blue cation with nitrate (as dihydrate), chloride (as pentahydrate) and thiocyanate counter-ions were generated by application of the invariom formalism and examined. The so-obtained charge density distributions were analyzed using the QTAIM formalism to yield bond topological and atomic properties. The atomic charges on the methylene blue cation indicate a delocalized charge distribution; only a small positive charge on the sulfur atom was found. Electrostatic potentials mapped onto iso-surfaces of electron density for the cations, and for the methylene blue cations with anions, were compared. The effect of hydrogen disorder on the molecular electrostatic potential was investigated for the thiocyanate structure.

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