Simultaneous determination of rabeprazole enantiomers and their four metabolites after intravenous administration in beagle dogs by a stereoselective HPLC-MS/MS method and its application in pharmacokinetic studies

2016 ◽  
Vol 8 (6) ◽  
pp. 1405-1414 ◽  
Author(s):  
Na Cao ◽  
Lei Liu ◽  
Yuan-bin Hao ◽  
Li-li Sun ◽  
Qiao-gen Zou ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Intravenous Administration ◽  
Pharmacokinetic Studies ◽  
Beagle Dogs ◽  
Beagle Dog

A sensitive, rapid and stable HPLC-MS/MS method has been developed and validated for the determination of rabeprazole enantiomers and their four metabolites in beagle dog.

scholarly journals Quantitation of Apremilast in Beagle Dogs Plasma by UPLC-MS-MS and Its Application to Pharmacokinetic Studies

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Wei Xiong ◽  
Ling Wang ◽  
Haiyan Zhang ◽  
Xiaoqiu Tao ◽  
Xuehua Jiang ◽  
...  
Keyword(s):  
Internal Standard ◽  
Gradient Elution ◽  
Ultra Performance Liquid Chromatography ◽  
Ammonium Formate ◽  
Pharmacokinetic Studies ◽  
Beagle Dogs ◽  
Beagle Dog ◽  
Dog Plasma ◽  
Liquid Chromatography Tandem Mass

A sensitive and selective ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) method for the determination of apremilast in beagle dog plasma has been developed and successfully validated in the current study. Clopidogrel was employed as an internal standard (IS), and liquid-liquid extraction by tert-butylmethyl ether was used for sample preparation. Chromatographic separation was achieved on a UPLC BEH Shield RP18 column (50 mm × 2.1 mm, 1.7 μm) with 5 mM ammonium formate water and 5 mM ammonium formate methanol as the mobile phase with gradient elution. Calibration plots were linear in the range of 2–3000 ng/mL for apremilast in beagle dog plasma. Mean recoveries of apremilast in beagle dogs plasma ranged from 87.4% to 97.4%. The intrarun and interrun precision was less than 6% and 9%, respectively, with the accuracy between 92.4% and 101.1%. The method has also been successfully applied in the pharmacokinetics study of apremilast. The mean t1/2Z was 5.41 h for 30 mg·day−1 for beagle dogs after oral administration. The AUC0-t increased linearly from 3.51 to 1802.13  μ g   L − 1 ∗ h after administration of single doses.

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