Hydrophilic interaction liquid chromatography for separation and determination of pyrrolidinium ionic liquid cations

2016 ◽  
Vol 8 (4) ◽  
pp. 840-845 ◽  
Author(s):  
Chao Guan ◽  
Hong Yu
Keyword(s):  
Ionic Liquid ◽  
Liquid Chromatography ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Hydrophilic Interaction ◽  
Ultraviolet Detection ◽  
Pyrrolidinium Ionic Liquid

The separation and detection of pyrrolidinium cations by hydrophilic interaction liquid chromatography with indirect ultraviolet detection.

scholarly journals Determination of Ascorbic Acid, Total Ascorbic Acid, and Dehydroascorbic Acid in Bee Pollen Using Hydrophilic Interaction Liquid Chromatography-Ultraviolet Detection

Molecules ◽  
2020 ◽  
Vol 25 (23) ◽  
pp. 5696
Author(s):  
Meifei Zhu ◽  
Jian Tang ◽  
Xijuan Tu ◽  
Wenbin Chen
Keyword(s):  
Ascorbic Acid ◽  
Liquid Chromatography ◽  
Dehydroascorbic Acid ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Hydrophilic Interaction ◽  
Metaphosphoric Acid ◽  
Ultraviolet Detection ◽  
Bee Pollen ◽  
Nutritional Analysis

Ascorbic acid (AA) is one of the essential nutrients in bee pollen, however, it is unstable and likely to be oxidized. Generally, the oxidation form (dehydroascorbic acid (DHA)) is considered to have equivalent biological activity as the reduction form. Thus, determination of the total content of AA and DHA would be more accurate for the nutritional analysis of bee pollen. Here we present a simple, sensitive, and reliable method for the determination of AA, total ascorbic acids (TAA), and DHA in rape (Brassica campestris), lotus (Nelumbo nucifera), and camellia (Camellia japonica) bee pollen, which is based on ultrasonic extraction in metaphosphoric acid solution, and analysis using hydrophilic interaction liquid chromatography (HILIC)-ultraviolet detection. Analytical performance of the method was evaluated and validated, then the proposed method was successfully applied in twenty-one bee pollen samples. Results indicated that contents of AA were in the range of 17.54 to 94.01 µg/g, 66.01 to 111.66 µg/g, and 90.04 to 313.02 µg/g for rape, lotus, and camellia bee pollen, respectively. In addition, percentages of DHA in TAA showed good intra-species consistency, with values of 13.7%, 16.5%, and 7.6% in rape, lotus, and camellia bee pollen, respectively. This is the first report on the discriminative determination between AA and DHA in bee pollen matrices. The proposed method would be valuable for the nutritional analysis of bee pollen.

Determination of alkyl ammonium ionic liquid cations by hydrophilic interaction liquid chromatography and its application in analysis of environmental water

2018 ◽  
Vol 10 (24) ◽  
pp. 2812-2820 ◽  
Author(s):  
Zi-qiang Fan ◽  
Hong Yu ◽  
Ya-qi Cai
Keyword(s):  
Ionic Liquid ◽  
Liquid Chromatography ◽  
Environmental Water ◽  
Reversed Phase ◽  
Ion Pair ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Hydrophilic Interaction ◽  
Alkyl Ammonium

Analysis of alkyl ammonium ionic liquid cations in environmental water by the reversed phase ion-pair SPE combined with HILIC-IUV. (a) The chromatogram of alkyl ammonium cations; (b) the chromatogram of alkyl ammonium cations after enrichment.

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