Multilayer and multichannel membrane filtration for separation and preconcentration of trace analytes and its application in spectral analysis

2016 ◽  
Vol 8 (1) ◽  
pp. 129-135 ◽  
Author(s):  
Wei Li ◽  
Haiting Wang ◽  
Wei Jiang ◽  
Lei Wang ◽  
Ruoqiu Zhang ◽  
...  
Keyword(s):  
Spectral Analysis ◽  
Membrane Filtration ◽  
Enrichment Method ◽  
Filtration Enrichment ◽  
Separation And Preconcentration ◽  
Mixture Samples

A multilayer and multichannel membrane filtration-enrichment method was developed to simultaneously separate and enrich trace analytes in mixture samples.

Detection of Vibrio cholerae and Vibrio parahaemolyticus by molecular and culture based methods from source water to household container-stored water at the point-of-use in South African rural communities

2010 ◽  
Vol 61 (12) ◽  
pp. 3091-3101 ◽  
Author(s):  
V. M. Ntema ◽  
N. Potgieter ◽  
T. G. Barnard
Keyword(s):  
16S Rrna ◽  
Vibrio Cholerae ◽  
Vibrio Parahaemolyticus ◽  
Pcr Detection ◽  
Toxin Gene ◽  
Specific Method ◽  
Culturable Bacteria ◽  
Source Water ◽  
Enrichment Method ◽  
Filtration Enrichment

Detection methods for Vibrio cholerae and Vibrio parahaemolyticus which included the culture based approach with polymerase chain reaction (PCR) confirmation, PCR detection without enrichment and PCR with a pre-enrichment were developed and their performance evaluated. PCR assays targeted the SodB (V. cholerae species), Flae (V. parahaemolyticus species), 16S rRNA (Vibrio and Enterobacteriacea species) genes (Multiplex 1) and V. cholerae O1 and V. cholerae O139 rfb genes, ctxA (cholera toxin) gene and 16S rRNA gene (Multiplex 2). These methods were used to determine the occurrence of selected Vibrios in source water as well as in household container-stored water. The combination of filtration, enrichment and PCR method provided a sensitive and specific method for the detection of selected Vibrios in water samples. The PCR with a pre-enrichment method detected as few as 4–10 cfu/100 mL of selected Vibrios and PCR detection without the enrichment method detected as few as 40–100 cfu/100 mL of selected Vibrios. The inclusion of an enrichment period allows detection of culturable bacteria. As an application of the developed methods, V. cholerae and V. parahaemolyticus were detected in the source water used by the population and in the water-storage containers. The results indicate that Vibrio species in the containers could have originated from the source water and survive in biofilms inside the containers.

COCHLIOBOLUS SATIVUS: VI. ISOLATION OF NUTRITIONALLY EXACTING MUTANTS BY FILTRATION ENRICHMENT

1962 ◽  
Vol 40 (10) ◽  
pp. 1293-1297 ◽  
Author(s):  
R. D. Tinline
Keyword(s):  
Mass Transfer ◽  
Comparative Studies ◽  
Minimal Medium ◽  
Cochliobolus Sativus ◽  
Complete Medium ◽  
Differential Growth ◽  
Enrichment Method ◽  
Filtration Enrichment ◽  
Hyphal Tip ◽  
Methods Of Isolation

Nutritionally exacting mutants of Cochliobolus sativus, a fungus with multinucleate, multicellular spores, were readily isolated by a filtration enrichment method. The method is similar to one described by Fries and is based upon the differential growth of auxotrophs and prototrophs in minimal medium. Most of the prototrophic propagules were removed by filtration. Propagules in the filtrate were concentrated by centrifugation, plated on complete medium, and subsequently tested for nutritional requirements.In comparative studies on four methods of isolation, namely, total isolation, mass transfer; total isolation, hyphal-tip transfer; filtration enrichment, mass transfer; and filtration enrichment, hyphal-tip transfer, the yield of auxotrophic mutants was 0, 0.04, 1.16, and 1.83%, respectively.

Separation and Preconcentration of Sudan Blue II Using Membrane Filtration and UV-Visible Spectrophotometric Determination in River Water and Industrial Wastewater Samples

2015 ◽  
Vol 98 (1) ◽  
pp. 213-217 ◽  
Author(s):  
Yunus Emre Unsal ◽  
Mustafa Tuzen ◽  
Mustafa Soylak
Keyword(s):  
Spectrophotometric Determination ◽  
Industrial Wastewater ◽  
Membrane Filtration ◽  
Matrix Effects ◽  
Membrane Filter ◽  
Cellulose Acetate Membrane ◽  
Separation And Preconcentration ◽  
Wastewater Samples ◽  
Working Media

Abstract A new separation and preconcentration method based on adsorption on a cellulose acetate membrane filter and elution with ethanol was established for the UV-Vis spectrophotometric determination of Sudan blue II. Various analytical parameters such as pH of working media, flow rates of solutions, and sample volumes were optimized. Matrix effects of concomitants were investigated for the quantitative recovery values of Sudan blue II. The preconcentration factor was200. LOD was calculated as 0.96 μg/L. RSD was5.1%. The optimized procedure was applied to the spectrophotometric determination of Sudan blue II in river and industrial wastewater samples from oil and dye products.

Separation and Preconcentration of Actinides by a Water-Soluble Oxine Polymer Using Membrane Filtration

1989 ◽  
Vol 46 (1) ◽  
Author(s):  
A. P. NOVIKOV ◽  
V. M. SHKINEV ◽  
B. YA. SPIVAKOV ◽  
B. F. MYASOEDOV ◽  
Κ. E. GECKELER ◽  
...  
Keyword(s):  
Membrane Filtration ◽  
Water Soluble ◽  
Separation And Preconcentration

scholarly journals Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum rifai

1999 ◽  
Vol 30 (1) ◽  
pp. 43-46
Author(s):  
Ana Maria R. Cassiolato ◽  
Itamar Soares de Melo
Keyword(s):  
Growth Rate ◽  
Trichoderma Harzianum ◽  
Genetic Research ◽  
Wild Type ◽  
Enrichment Method ◽  
Isolation Technique ◽  
Auxotrophic Mutants ◽  
Enrichment Technique ◽  
Filtration Enrichment ◽  
Similar Growth

The isolation of genetic markers, like drug resistance and auxotrophy, is a laborious but important step in genetic research. The isolation of auxotrophic mutants of Trichoderma harzianum using the filtration enrichment technique was more effective than using the total isolation technique. Most of 12 auxotrophic mutants exhibited similar growth rate and higher sporulation when compared with the wild type, but only two mutants (TWS-410 and TW5-523) could grow in 500µg/L of benomyl.

Digital image colorimetry coupled with a multichannel membrane filtration-enrichment technique to detect low concentration dyes

2016 ◽  
Vol 8 (14) ◽  
pp. 2887-2894 ◽  
Author(s):  
Wei Li ◽  
Ruoqiu Zhang ◽  
Haiting Wang ◽  
Wei Jiang ◽  
Lei Wang ◽  
...  
Keyword(s):  
Mobile Phone ◽  
Digital Image ◽  
Membrane Filtration ◽  
Low Concentration ◽  
Enrichment Technique ◽  
Filtration Enrichment

A sensitive and accessible digital image colorimetry method was developed based on the multichannel membrane filtration-enrichment technique utilizing a mobile phone as the analytical detector.

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