An aptasensor based on fluorescence resonance energy transfer for multiplexed pathogenic bacteria determination

2016 ◽  
Vol 8 (6) ◽  
pp. 1390-1395 ◽  
Author(s):  
Nuo Duan ◽  
Wenhui Gong ◽  
Zhouping Wang ◽  
Shijia Wu
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Pathogenic Bacteria ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Bacteria Detection ◽  
Fluorescence Resonance

A multiplexed FRET-based aptasensor was developed for simultaneous bacteria detection using multicolor dyes as donors and CNPs as the sole acceptor.

scholarly journals A High-Throughput Fluorescence Resonance Energy Transfer-Based Assay for DNA Ligase

2011 ◽  
Vol 16 (5) ◽  
pp. 486-493 ◽  
Author(s):  
Adam B. Shapiro ◽  
Ann E. Eakin ◽  
Grant K. Walkup ◽  
Olga Rivin
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
High Throughput ◽  
High Throughput Screening ◽  
Pathogenic Bacteria ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Dna Ligase ◽  
Phosphodiester Bond ◽  
Fluorescence Resonance

DNA ligase is the enzyme that catalyzes the formation of the backbone phosphodiester bond between the 5′-PO4 and 3′-OH of adjacent DNA nucleotides at single-stranded nicks. These nicks occur between Okazaki fragments during replication of the lagging strand of the DNA as well as during DNA repair and recombination. As essential enzymes for DNA replication, the NAD+-dependent DNA ligases of pathogenic bacteria are potential targets for the development of antibacterial drugs. For the purposes of drug discovery, a high-throughput assay for DNA ligase activity is invaluable. This article describes a straightforward, fluorescence resonance energy transfer–based DNA ligase assay that is well suited for high-throughput screening for DNA ligase inhibitors as well as for use in enzyme kinetics studies. Its use is demonstrated for measurement of the steady-state kinetic constants of Haemophilus influenzae NAD+-dependent DNA ligase and for measurement of the potency of an inhibitor of this enzyme.

Detection of Pathogenic Bacteria in Milk and Whey Samples using A Fluorescence Resonance Energy Transfer Aptasensor based on Cerium Oxide Nanoparticles

2022 ◽  
Author(s):  
Sena Ghayyem ◽  
Farnoush Faridbod
Keyword(s):  
Energy Transfer ◽  
Cerium Oxide ◽  
Fluorescence Resonance Energy Transfer ◽  
Pathogenic Bacteria ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Energy Donor ◽  
Fluorescence Resonance

Herein, we present a facile and sensitive fluorescence resonance energy transfer (FRET) aptasensor for detection of pathogenic bacteria, where Antibiotic-functionalized cerium oxide nanoparticles were served as an energy donor and...

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