Extraction of plant growth regulators present in Kappaphycus alvarezii sap using imidazolium based ionic liquids: detection and quantification by using HPLC-DAD technique

2015 ◽  
Vol 7 (21) ◽  
pp. 9064-9067 ◽  
Author(s):  
Arun Kumar Das ◽  
Kamalesh Prasad
Keyword(s):  
Acetic Acid ◽  
Ionic Liquids ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Kappaphycus Alvarezii ◽  
Hydrophobic Ionic Liquids ◽  
Indole 3 Acetic Acid ◽  
Detection And Quantification

Among three different hydrophobic ionic liquids, [Bmim][PF6] was found to extract selectively trans-zeatin and indole-3-acetic acid from Kappaphycus alvarezii sap.

Monoclonal antibodies to plant growth regulators. II. Indole-3-acetic acid

Planta ◽  
1985 ◽  
Vol 166 (3) ◽  
pp. 389-393 ◽  
Author(s):  
R. Mertens ◽  
J. Eberle ◽  
A. Arnscheidt ◽  
A. Ledebur ◽  
E. W. Weiler
Keyword(s):  
Acetic Acid ◽  
Monoclonal Antibodies ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Indole 3 Acetic Acid

scholarly journals Effect of Gibberellic Acid and Indole 3-Acetic Acid on Seed Germination Performance of Horse gram (Macrotyloma uniflorum) Lam (Verdc)

2016 ◽  
Vol 1 (2) ◽  
pp. 36 ◽  
Author(s):  
J. Lalitha ◽  
Haseena Rafath ◽  
M. Subash
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Plant Growth ◽  
Gibberellic Acid ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Germination Percentage ◽  
Potential Growth ◽  
Macrotyloma Uniflorum ◽  
Indole 3 Acetic Acid

The experiment was undertaken with an objective to investigate the effect of various concentrations of plant growth regulators, i.e., Gibberellic acid (GA3) and Indole 3-acetic acid (IAA) on seed germination of Macrotyloma uniflorum. Seeds were soaked for 12 hours in different concentrations substances (1.0, 2.0, 3.0 and 4.0 mg/L) of IAA, GA3 and control set was soaked only in distilled water.  Three replicates of each treatment with fifty seeds per replicate were arranged for precise physiological analysis. Significant variation was found in all aspects after analysis of variance (ANOVA) of each mean value. After two weeks of seed soaking, it was noted that germination percentages were significantly accelerated by lower concentrations (1 and 2 mg) of used hormones. Amongst the two potential growth regulators, 2 mg/L was found most effective because it showed highest germination percentage for IAA (93%) and GA3 (88%). A great deal of information relating to seed germination practices shows that these plant growth regulators were efficient in overcoming dormancy leading to rapid seed germination. IAA was selected as best hormone in this study, which showed highest seed germination (93%). 

scholarly journals Effect of maize root exudates on indole-3-acetic acid production by rice endophytic bacteria under influence of L-tryptophan

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 112 ◽  
Author(s):  
Arun Karnwal ◽  
Aradhana Dohroo
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Acid Production ◽  
Growth Promotion ◽  
Bacterial Species ◽  
Critical Role ◽  
Acetic Acid Production ◽  
Indole 3 Acetic Acid

Background: It is assumed that plant growth regulators produced by beneficial bacterial species could also influence plant growth. IAA is a major plant growth regulator responsible for stimulation of plant growth. There are several microorganisms which are naturally responsible for L- tryptophan metabolism. Methods: In total, 56 indigenous morphologically distinct isolates from rice roots were selected and subsequently characterized with biochemical tests, 16S rRNA sequencing and plant growth promoting activities. Pseudomonas fluorescens RE1 (GenBank: MF102882.1) and RE17 (GenBank: MF103672.1) endophytes resulted in better PGP activity against the other 54 isolates. Both endophytes were tested to screen indole-3-acetic acid production ability in pure culture conditions with L-tryptophan at 0, 50, 100, 200 and 500µg/ml concentrations. Results: P. fluorescens RE1 was recorded efficient for indole production in comparison to P. fluorescens RE17 at various L-tryptophan concentrations. P. fluorescens RE1 was shown to produce between 0.8 µg/ml and 11.5µg/ml of indole at various tryptophan concentrations, while RE17 produced between 1.2µg/ml and 10.2µg/ml. At 200 and 500µg/ml tryptophan concentration, P. fluorescens RE17 produced 7.4pmol/ml and 9.3pmol/ml IAA, respectively.  Conclusions: Inoculation of maize seed with P. fluorescens RE1 and RE17 showed a significantly higher level of IAA production in comparison to non-inoculated seeds. Current study outcomes proved that plant growth regulators produced by Pseudomonas species could also play a critical role in plant growth promotion.

Deep eutectic solvent-based liquid-phase microextraction for detection of plant growth regulators in edible vegetable oils

2016 ◽  
Vol 8 (17) ◽  
pp. 3511-3516 ◽  
Author(s):  
Ting Tan ◽  
Zhan Li ◽  
Xuejin Mao ◽  
Yiqun Wan ◽  
Hongdeng Qiu
Keyword(s):  
Acetic Acid ◽  
Liquid Phase ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Vegetable Oils ◽  
Growth Regulators ◽  
Deep Eutectic Solvent ◽  
Liquid Phase Microextraction ◽  
Indole 3 Acetic Acid ◽  
Edible Vegetable Oil

A series of tunable DESs were prepared and used for the liquid-phase microextraction of plant growth regulators, including indole-3-acetic acid, indole-3-butyric acid and 4-iodophenoxyacetic acid, from a multi-component edible vegetable oil matrix.

scholarly journals Plant Growth Regulators Improve the Production of Volatile Organic Compounds in Two Rose Varieties

Plants ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 35 ◽  
Author(s):  
Mohammed Ibrahim ◽  
Manjree Agarwal ◽  
Jeong Oh Yang ◽  
Muslim Abdulhussein ◽  
Xin Du ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Volatile Organic Compounds ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Organic Compounds ◽  
Growth Regulators ◽  
Naphthalene Acetic Acid ◽  
Flame Ionization ◽  
Three Phase ◽  
Volatile Organic

The study focused on the influence of the plant growth regulators (PGRs) benzyladenine (BA) and naphthalene acetic acid (NAA) on the production of volatile organic compounds (VOCs) from the flowers of two modern rose varieties, Hybrid Tea and Floribunda. Thirty-six plants of Hybrid Tea and Floribunda were tested. Benzyladenine and naphthalene acetic acid were applied at 0, 100 and 200 mg/L to both rose varieties. Gas chromatography, coupled with flame ionization detection and mass spectrometry, was used to analyze and identify the volatile organic compounds from the flowers. A three-phase fiber 50/30 µm divinylbenzene/carboxen/polydimethylsiloxane was used to capture VOCs, at 2, 4 and 8 weeks, and 4 weeks was selected as it had the highest peak area. In total, 81 and 76 VOCs were detected after treatment of both rose varieties with BA and NAA, respectively. In addition, 20 compounds, which had significant differences between different treatments, were identified from both rose varieties. The majority of VOCs were extracted after the application of 200 mg (BA and NAA) /L of formulation, and four important compounds, cis-muurola-4(141)5-diene, y-candinene, y-muurolene and prenyl acetate, increased significantly compared to the controls. These compounds are commercially important aroma chemicals. This study used the rapid and solvent-free SPME method to show that BA and NAA treatments can result in significant VOC production in the flowers of two rose varieties, enhancing the aromatic value of the flowers. This method has the potential to be applied to other valuable aromatic floricultural plant species.

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