Monoclonal antibody-based cross-reactive sandwich ELISA for the detection of Salmonella spp. in milk samples

2015 ◽  
Vol 7 (21) ◽  
pp. 9047-9053 ◽  
Author(s):  
Xiaoling Wu ◽  
Wenbin Wang ◽  
Liqiang Liu ◽  
Hua Kuang ◽  
Chuanlai Xu
Keyword(s):  
Monoclonal Antibody ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Sandwich Elisa ◽  
Periodate Oxidation ◽  
Salmonella Spp ◽  
Milk Samples

An immunogen consisting ofSalmonellalipopolysaccharide and bovine serum albumin was prepared by periodate oxidation.

Enantio and diastereoselectivity in the periodate oxidation of sulfides catalyzed by bovine serum albumin. 2

1986 ◽  
Vol 51 (6) ◽  
pp. 891-895 ◽  
Author(s):  
Stefano Colonna ◽  
Stefano Banfi ◽  
Rita Annunziata ◽  
Luigi Casella
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Periodate Oxidation ◽  
Oxidation Of Sulfides

scholarly journals Study of the transit of an integral membrane protein from secretory granules through the plasma membrane of secreting rat basophilic leukemia cells using a specific monoclonal antibody.

1986 ◽  
Vol 102 (2) ◽  
pp. 516-522 ◽  
Author(s):  
J S Bonifacino ◽  
P Perez ◽  
R D Klausner ◽  
I V Sandoval
Keyword(s):  
Monoclonal Antibody ◽  
Bovine Serum Albumin ◽  
Membrane Protein ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Secretory Granules ◽  
Integral Membrane Protein ◽  
Rat Basophilic Leukemia Cells ◽  
Rbl Cells ◽  
Basophilic Leukemia

The monoclonal antibody 5G10 reacted specifically with an 80-kD integral membrane protein in rat basophilic leukemia (RBL) cells. Immunofluorescence microscopy studies of RBL cells, fixed and permeabilized, revealed that the 80-kD protein was located in the membrane of cytoplasmic vesicles. The vesicles were identified as secretory granules by their content in immunoreactive serotonin. Expression of the 5G10 antigen on the surface of unstimulated RBL cells was low. However, RBL cells stimulated to secrete with anti-dinitrophenyl IgE followed by dinitrophenyl-bovine serum albumin or with the Ca2+ ionophore A-23187 displayed an increased expression of the antigen on their surface. Surface exposure of the 5G10 antigen was maximal at 5 min after stimulation of secretion. Removal of dinitrophenyl-bovine serum albumin from the incubation medium resulted in internalization of 50% of the antigen within 10 min.

The Synthesis and Identification of a Ciprofloxacin Artificial Antigen

2013 ◽  
Vol 830 ◽  
pp. 314-318
Author(s):  
Long Xu ◽  
Li Xin Zhu ◽  
Ren Rong Liu ◽  
Yan Fan ◽  
Wei Meng
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Sodium Periodate ◽  
Periodate Oxidation ◽  
Uv Absorption ◽  
Absorption Method ◽  
Oxidation Method ◽  
Artificial Antigen

The artificial antigen ciprofloxacin-bovine serum albumin (CPFX-BSA) and ciprofloxacin-ovalbumin (CPFX-OVA) were synthesized by carbodiimide (EDC) method and sodium periodate oxidation method, respectively. These two kinds of antigens were identified by the UV absorption method and the animal immunization test, which showed that they were synthesized successfully. The successful synthesis of ciprofloxacin artificial antigen is important to establish immunoassay of ciprofloxacin.

Pregnancy-blocking progesterone antibody targets specifically the uterus through its progesterone-binding sites

1990 ◽  
Vol 4 (3) ◽  
pp. 283-291 ◽  
Author(s):  
M.-W. Wang ◽  
A. Whyte ◽  
R. B. Heap ◽  
M. J. Taussig
Keyword(s):  
Monoclonal Antibody ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Binding Sites ◽  
Bovine Serum ◽  
Passive Immunization ◽  
Uterine Epithelium ◽  
High Affinity ◽  
Specific Targeting

ABSTRACT Passive immunization with a mouse monoclonal antibody against progesterone, designated DB3, blocks pregnancy in several species. We have previously reported that DB3 localizes in the mouse uterine epithelium shortly before normal implantation. This phenomenon is pregnancy dependent and specific for the progesterone antibody. In this study we demonstrate that DB3 is present in the lumen of the uterus 36 h after an i.p. injection; this correlates with the time of maximum antibody reaction on the uterine epithelium. Incubation of DB3 with free progesterone, progesterone-hemisuccinate or progesterone—bovine serum albumin before administration prevented its localization on the epithelium, indicating that the localization requires free progesterone-binding sites and thus probably depends upon progesterone binding. In addition, studies in vitro show that DB3 can effectively bind to progesterone carried by high-affinity progesterone-binding protein purified from coypu plasma. We suggest that specific targeting of DB3 may be through progesterone associated with a progesterone-binding molecule on the membrane of the uterine epithelia. This may be an important part of the mechanism of antibody action against implantation.

Sign in / Sign up

Export Citation Format

Share Document