A fluorescence immunochromatographic assay for rapid and sensitive detection of human prealbumin in serum

2015 ◽  
Vol 7 (20) ◽  
pp. 8683-8688 ◽  
Author(s):  
Yajie Yang ◽  
Chaohui Li ◽  
Wei Wang ◽  
Tingting Dong ◽  
Yonghua Xiong ◽  
...  
Keyword(s):  
Test Strip ◽  
Sensitive Detection ◽  
Immunochromatographic Assay ◽  
Human Prealbumin

Schematic of the sandwich FMs-LFIA test strip.

scholarly journals A Lateral Flow Strip Based on a Truncated Aptamer-Complementary Strand for Detection of Type-B Aflatoxins in Nuts and Dried Figs

Toxins ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 136 ◽  
Author(s):  
Zhilei Zhao ◽  
He Wang ◽  
Wenlei Zhai ◽  
Xiaoyuan Feng ◽  
Xia Fan ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Cdna Probe ◽  
Test Strip ◽  
Lateral Flow ◽  
Sensitive Detection ◽  
Detection Methods ◽  
Complementary Strand ◽  
Type B ◽  
Experimental Conditions ◽  
Lateral Flow Strip

Type-B aflatoxins (AFB1 and AFB2) frequently contaminate food, especially nuts and fried figs, and seriously threaten human health; hence, it is necessary for the newly rapid and sensitive detection methods to prevent the consumption of potentially contaminated food. Here, a lateral flow aptasensor for the detection of type-B aflatoxins was developed. It is based on the use of fluorescent dye Cy5 as a label for the aptamer, and on the competition between type-B aflatoxins and the complementary DNA of the aptamer. This is the first time that the complementary strand of the aptamer has been used as the test line (T-line) to detect type-B aflatoxins. In addition, the truncated aptamer was used to improve the affinity with type-B aflatoxins in our study. Therefore, the lengths of aptamer and cDNA probe were optimized as key parameters for higher sensitivity. In addition, binding buffer and organic solvent were investigated. The results showed that the best pair for achieving improved sensitivity and accuracy in detecting AFB1 was formed by a shorter aptamer (32 bases) coupled with the probe complementary to the AFB1 binding region of the aptamer. Under the optimal experimental conditions, the test strip showed an excellent linear relationship in the range from 0.2 to 20 ng/mL with a limit of detection of 0.16 ng/mL. This aptamer-based strip was successfully applied to the determination of type-B aflatoxins in spiked and commercial peanuts, almonds, and dried figs, and the recoveries of the spiked samples were from 93.3%−112.0%. The aptamer-complementary strand-based lateral flow test strip is a potential alternative tool for the rapid and sensitive detection of type-B aflatoxins in nuts and dried figs. It is of help for monitoring aflatoxins to avoid the consumption of unsafe food.

Hierarchical Flowerlike Gold Nanoparticles Labeled Immunochromatography Test Strip for Highly Sensitive Detection ofEscherichia coliO157:H7

Langmuir ◽  
2015 ◽  
Vol 31 (19) ◽  
pp. 5537-5544 ◽  
Author(s):  
Lei Zhang ◽  
Youju Huang ◽  
Jingyun Wang ◽  
Yun Rong ◽  
Weihua Lai ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Test Strip ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Ultra technically-simple and sensitive detection for Salmonella Enteritidis by immunochromatographic assay based on gold growth

Food Control ◽  
2018 ◽  
Vol 84 ◽  
pp. 536-543 ◽  
Author(s):  
Tong Bu ◽  
Qiong Huang ◽  
Lingzhi Yan ◽  
Lunjie Huang ◽  
Mengyue Zhang ◽  
...  
Keyword(s):  
Salmonella Enteritidis ◽  
Sensitive Detection ◽  
Immunochromatographic Assay

Development of monoclonal antibody and lateral test strip for sensitive detection of clenbuterol and related β2-agonists in urine samples

2015 ◽  
Vol 27 (1) ◽  
pp. 111-127 ◽  
Author(s):  
Gabriel Wafula Khaemba ◽  
Bitange Nipa Tochi ◽  
Daniel Mukunzi ◽  
Isanga Joel ◽  
Lingling Guo ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Test Strip ◽  
Urine Samples ◽  
Sensitive Detection ◽  
Β2 Agonists

scholarly journals Fluorescent fullerene nanoparticle-based lateral flow immunochromatographic assay for rapid quantitative detection of C-reactive protein

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Kyung Mi Park ◽  
Da Jung Chung ◽  
Mijin Choi ◽  
Taejoon Kang ◽  
Jinyoung Jeong
Keyword(s):  
Chemical Species ◽  
Test Strip ◽  
Disease Diagnosis ◽  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Quantitative Detection ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Diagnosis And Prognosis ◽  
Lateral Flow Immunochromatographic Assay

Abstract A fluorescent fullerene nanoparticle (NP)-based lateral flow immunochromatographic assay (LFIA) was developed for the rapid and quantitative detection of C-reactive protein (CRP) in serum. The polyclonal CRP-antibody-conjugated fullerene NPs were simply prepared by 1-ethyl-3-(3-dimethyllaminopropyl)-carbodiimide hydrochloride coupling after carboxylation of fluorescent fullerene NPs. By applying the CRP-antibody-conjugated fullerene NPs to a lateral flow test strip, quantitative analysis of CRP in serum was possible at a concentration range of 0.1–10 ng/ml within 15 min. We anticipate that this novel fluorescent fullerene NP-based LFIA can be useful for the rapid and accurate sensing of biological and chemical species, contributing to the disease diagnosis and prognosis, environmental monitoring, and food safety.

Size-dependent quantum dot nanobeads based immunochromatographic assay for sensitive detection of Ochratoxin A

Toxicon ◽  
2019 ◽  
Vol 158 ◽  
pp. S18-S19
Author(s):  
Yuankui Leng ◽  
Hong Duan ◽  
Xiaolin Huang ◽  
Yanna Shao ◽  
Linyan Zheng ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Ochratoxin A ◽  
Sensitive Detection ◽  
Immunochromatographic Assay ◽  
Size Dependent

Upconversion luminescence nanoparticles-based lateral flow immunochromatographic assay for cephalexin detection

2014 ◽  
Vol 2 (45) ◽  
pp. 9637-9642 ◽  
Author(s):  
Chunyan Liu ◽  
Wei Ma ◽  
Zhenyu Gao ◽  
Jiayi Huang ◽  
Yi Hou ◽  
...  
Keyword(s):  
Upconversion Luminescence ◽  
Lateral Flow ◽  
Sensitive Detection ◽  
Immunochromatographic Assay ◽  
Core Shell ◽  
Shell Nanoparticles ◽  
Core Shell Nanoparticles ◽  
Lateral Flow Immunochromatographic Assay

Upconversion luminescence core–shell nanoparticles were used as antibody labels in a lateral flow immunochromatographic assay for the sensitive detection of cephalexin.

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