A novel hybrid mesostructured silica for the solid-phase extraction of estrogenic hormones from waters

2015 ◽  
Vol 7 (11) ◽  
pp. 4740-4749 ◽  
Author(s):  
Judith Gañán ◽  
Sonia Morante-Zarcero ◽  
Damián Pérez-Quintanilla ◽  
Isabel Sierra
Keyword(s):  
Mesoporous Silica ◽  
Solid Phase Extraction ◽  
Stationary Phase ◽  
Solid Phase ◽  
Phase Extraction ◽  
One Pot ◽  
Mesostructured Silica ◽  
One Pot Synthesis ◽  
First Time

SBA-15-C18 mesoporous silica was prepared by one-pot synthesis. The material was applied for the first time as a stationary phase for SPE. Good recoveries were obtained for the determination of twelve EDCs in waters by HPLC.

A multidrug LC–MS/MS method for the determination of five immunosuppressants in oral fluid

Bioanalysis ◽  
2019 ◽  
Vol 11 (16) ◽  
pp. 1509-1521 ◽  
Author(s):  
Lucía Paniagua-González ◽  
Elena Lendoiro ◽  
Esteban Otero-Antón ◽  
Esther Molina-Pérez ◽  
Evaristo Varo-Pérez ◽  
...  
Keyword(s):  
Therapeutic Drug Monitoring ◽  
Solid Phase Extraction ◽  
Mobile Phase ◽  
Oral Fluid ◽  
Solid Phase ◽  
Therapeutic Drug ◽  
Phase Extraction ◽  
Trough Concentrations ◽  
First Time

Aim: This study aimed: to develop and validate an LC–MS/MS method for mycophenolic acid, tacrolimus, sirolimus, everolimus and cyclosporin A in oral fluid (OF), as an essential tool to study the usefulness of OF as an alternative matrix for immunossuppressants’ therapeutic drug monitoring; and to find the best OF collector for these analytes. Materials & Methods: Chromatographic separation was achieved using an XBridge® Shield RP18 analytical column maintained at 65ºC, using 2 mM ammonium formate and 0.1% formic acid in water (A) and acetonitrile (B) as mobile phase. OF sample was extracted with solid phase extraction after sonication and protein precipitation. Results & Conclusions: Method validation met all the acceptance criteria. LODs were 0.05–1 ng/ml, and LOQs 0.1–5 ng/ml. Silanized tubes offered the best recoveries. The method was successfully applied to 31 OF specimens, describing everolimus detection in OF for the first time. Conclusion: The proposed method is sensitive enough for the detection of OF trough concentrations in patients receiving immunosuppressants when using an appropriate OF collector.

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