Development of a colloidal gold-based lateral-flow immunoassay for the rapid detection of phenylethanolamine A in swine urine

2015 ◽  
Vol 7 (10) ◽  
pp. 4130-4137 ◽  
Author(s):  
Mingyan Dai ◽  
Yunfei Gong ◽  
Amin Liu ◽  
Lulu Zhang ◽  
Junxiao Lin ◽  
...  
Keyword(s):  
Colloidal Gold ◽  
Rapid Detection ◽  
Growth Promotion ◽  
Lateral Flow ◽  
Feed Additives ◽  
Lateral Flow Immunoassay ◽  
Adrenergic Agonists ◽  
Swine Urine ◽  
The Family

Phenylethanolamine A (PEAA) is a newly emerged phenethanolamine member of the family of β-adrenergic agonists illegally used as feed additives for growth promotion.

Development of a colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of clenbuterol and ractopamine in swine urine

2009 ◽  
Vol 395 (8) ◽  
pp. 2591-2599 ◽  
Author(s):  
Ming-Zhou Zhang ◽  
Min-Zi Wang ◽  
Zong-Lun Chen ◽  
Jie-Hong Fang ◽  
Mei-Ming Fang ◽  
...  
Keyword(s):  
Colloidal Gold ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Swine Urine

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

scholarly journals A simple and compact smartphone-based device for the quantitative readout of colloidal gold lateral flow immunoassay strips

2018 ◽  
Vol 266 ◽  
pp. 63-70 ◽  
Author(s):  
Wei Xiao ◽  
Caihong Huang ◽  
Fei Xu ◽  
Junjie Yan ◽  
Hongfen Bian ◽  
...  
Keyword(s):  
Colloidal Gold ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

scholarly journals Development of a Prototype Lateral Flow Immunoassay for Rapid Detection of Staphylococcal Protein A in Positive Blood Culture Samples

Diagnostics ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 794
Author(s):  
Arpasiri Srisrattakarn ◽  
Patcharaporn Tippayawat ◽  
Aroonwadee Chanawong ◽  
Ratree Tavichakorntrakool ◽  
Jureerut Daduang ◽  
...  
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Rapid Detection ◽  
Protein A ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Staphylococcal Protein A ◽  
Highly Sensitive ◽  
Sophisticated Equipment ◽  
Higher Sensitivity

Bloodstream infection (BSI) is a major cause of mortality in hospitalized patients worldwide. Staphylococcus aureus is one of the most common pathogens found in BSI. The conventional workflow is time consuming. Therefore, we developed a lateral flow immunoassay (LFIA) for rapid detection of S. aureus-protein A in positive blood culture samples. A total of 90 clinical isolates including 58 S. aureus and 32 non-S. aureus were spiked in simulated blood samples. The antigens were extracted by a simple boiling method and diluted before being tested using the developed LFIA strips. The results were readable by naked eye within 15 min. The sensitivity of the developed LFIA was 87.9% (51/58) and the specificity was 93.8% (30/32). When bacterial colonies were used in the test, the LFIA provided higher sensitivity and specificity (94.8% and 100%, respectively). The detection limit of the LFIA was 107 CFU/mL. Initial evaluation of the LFIA in 20 positive blood culture bottles from hospitals showed 95% agreement with the routine methods. The LFIA is a rapid, simple and highly sensitive method. No sophisticated equipment is required. It has potential for routine detection particularly in low resource settings, contributing an early diagnosis that facilitates effective treatment and reduces disease progression.

scholarly journals Development of Colloidal Gold‐Based Lateral Flow Immunoassay for Rapid Qualitative and SemiQuantitative Analysis of Ustiloxins A and B in Rice Samples

Toxins ◽  
2017 ◽  
Vol 9 (3) ◽  
pp. 79 ◽  
Author(s):  
Xiaoxiang Fu ◽  
Rushan Xie ◽  
Jian Wang ◽  
Xiaojiao Chen ◽  
Xiaohan Wang ◽  
...  
Keyword(s):  
Colloidal Gold ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Semiquantitative Analysis ◽  
Rice Samples

Development of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Olaquindox Residues

2011 ◽  
Vol 59 (17) ◽  
pp. 9319-9326 ◽  
Author(s):  
Chunmei Song ◽  
Qingtang Liu ◽  
Aimin Zhi ◽  
Jifei Yang ◽  
Yubao Zhi ◽  
...  
Keyword(s):  
Colloidal Gold ◽  
Rapid Detection ◽  
Lateral Flow

Development of a nucleic acid lateral flow immunoassay (NALFIA) for reliable, simple and rapid detection of the methicillin resistance genes mecA and mecC

2017 ◽  
Vol 200 ◽  
pp. 101-106 ◽  
Author(s):  
Constanze Seidel ◽  
Sonja Peters ◽  
Erik Eschbach ◽  
Andrea T. Feßler ◽  
Boris Oberheitmann ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Resistance Genes ◽  
Rapid Detection ◽  
Methicillin Resistance ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

A Lateral Flow Immunoassay for the Rapid Detection of Ochratoxin A in Wine and Grape Must

2012 ◽  
Vol 60 (46) ◽  
pp. 11491-11497 ◽  
Author(s):  
Laura Anfossi ◽  
Cristina Giovannoli ◽  
Gianfranco Giraudi ◽  
Flavia Biagioli ◽  
Cinzia Passini ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Rapid Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Grape Must
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