Monitoring the clandestine synthesis of methamphetamine in real-time with ambient sampling, portable mass spectrometry

2015 ◽  
Vol 7 (17) ◽  
pp. 7156-7163 ◽  
Author(s):  
Adam E. O'Leary ◽  
Seth E. Hall ◽  
Kyle E. Vircks ◽  
Christopher C. Mulligan
Keyword(s):  
Mass Spectrometry ◽  
Real Time ◽  
Mass Spectrometer ◽  
Ambient Ionization ◽  
Reaction Monitoring ◽  
Portable Mass Spectrometer ◽  
Ionization Methods

In this paper, an ambient sampling, portable mass spectrometer coupled to a selection on ambient ionization methods was used to perform real-time reaction monitoring of clandestine methamphetamine syntheses.

scholarly journals 662. Identification of Clinically Relevant Microbes with the MasSpec Pen

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S386-S387
Author(s):  
Sydney C Povilaitis ◽  
Ashish D Chakraborty ◽  
Rachel D Downey ◽  
Sarmistha Bhaduri Hauger ◽  
Livia Eberlin
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometer ◽  
Model Performance ◽  
Sample Surface ◽  
Clinical Analysis ◽  
Ambient Ionization ◽  
Rapid Identification ◽  
Negative Ion ◽  
Ionization Mass ◽  
Molecular Features

Abstract Background In the age of antimicrobial resistance, rapid identification of infectious agents is critical for antimicrobial stewardship and effective therapy. To this end, ambient ionization mass spectrometry techniques have been applied for rapid identification of microbes directly from culture isolates. We have developed a handheld, mass spectrometry-based device, the MasSpec Pen, that permits direct molecular analysis of a biological sample in seconds (Scheme 1). Here, we employ the MasSpec Pen to identify clinically relevant microbes directly from culture isolates. Methods Staphylococcus aureus, Staphylococcus epidermidis, Group A and B Streptococcus, Kingella kingae (K.k), and Pseudomonas aeruginosa (P.a) were cultured on 5% sheep’s blood nutrient agar at 37 °C overnight. Colonies were transferred to a glass slide where they were analyzed directly with the MasSpec Pen coupled to a Q Exactive mass spectrometer (Thermo Scientific) in negative ion mode. For MasSpec Pen analysis, a 10 µL droplet of water was held in contact with the sample surface for 3 seconds and then aspirated to the mass spectrometer for analysis. Data was normalized and the molecular features resulting from the analysis solvent and nutrient medium were removed. The least absolute shrinkage and selection operator (lasso) statistical method was used to build classification models for prediction of bacterial identity. Model performance was evaluated by leave-one-out cross-validation and a validation set of samples. Scheme 1: MasSpec Pen workflow Results Various small molecules were detected including metabolites and glycerophospholipid species. The mass spectral profiles for each species exhibited qualitative differences among them (Figure 1). Additionally, several quorum-sensing molecules were observed in P.a. including hydroxy-heptyl-quinoline (m/z 242.155). Lasso statistical classifiers were created to differentiate organisms at the level of Gram type, genus, and species with each model comprised of a sparse set of molecular features. Accuracies of 90% or greater were achieved for all lasso models and as high as 98% for the differentiation of Staphylococcus (Staph.) and Streptococcus (Strep.). Figure 1: Molecular profiles of species analyzed Figure 2: Statistical classification results Conclusion These results demonstrate the potential of the MasSpec Pen as a tool for clinical analysis of infected biospecimens. Disclosures Sydney C. Povilaitis, BA, MS Pen Technologies, Inc. (Other Financial or Material Support, Patent) Livia Eberlin, PhD, MS Pen Technolpogies, Inc. (Board Member, Shareholder)

The applicability of proton transfer reaction-mass spectrometry (PTR-MS) for determination of isocyanic acid (ICA) in work room atmospheres

2014 ◽  
Vol 16 (10) ◽  
pp. 2423-2431 ◽  
Author(s):  
Mikolaj Jan Jankowski ◽  
Raymond Olsen ◽  
Claus Jørgen Nielsen ◽  
Yngvar Thomassen ◽  
Paal Molander
Keyword(s):  
Mass Spectrometry ◽  
Proton Transfer ◽  
Real Time ◽  
Mass Spectrometer ◽  
Transfer Reaction ◽  
Proton Transfer Reaction ◽  
Reaction Mass ◽  
Isocyanic Acid

This study presents a real-time method to quantitatively determine isocyanic acid (ICA) in workroom air using a proton transfer reaction-mass spectrometer (PTR-MS).

scholarly journals Mobile mass spectrometry for water quality monitoring of organic species present in nuclear waste ponds

2018 ◽  
Vol 10 (48) ◽  
pp. 5827-5833 ◽  
Author(s):  
Boris Brkić ◽  
Stamatios Giannoukos ◽  
Stephen Taylor ◽  
Darren F. Lee
Keyword(s):  
Mass Spectrometry ◽  
Water Quality ◽  
Organic Compounds ◽  
Mass Spectrometer ◽  
Nuclear Waste ◽  
Water Quality Monitoring ◽  
Quality Monitoring ◽  
Organic Species ◽  
Portable Mass Spectrometer ◽  
Mobile Mass

This work uses a portable mass spectrometer (MS) for lab-based water quality monitoring of organic compounds, which are present in highly toxic nuclear waste ponds.

More than just heat: ambient ionization mass spectrometry for determination of the species of origin of processed commercial products—application to psychoactive pepper supplements

2016 ◽  
Vol 8 (7) ◽  
pp. 1646-1658 ◽  
Author(s):  
Ashton D. Lesiak ◽  
Rabi A. Musah
Keyword(s):  
Mass Spectrometry ◽  
Statistical Analysis ◽  
Real Time ◽  
Plant Species ◽  
Direct Analysis ◽  
Ambient Ionization ◽  
Ionization Mass ◽  
Commercial Products ◽  
Ambient Ionization Mass Spectrometry

Direct analysis in real time mass spectrometry coupled with statistical analysis tools were applied to deduce the plant species from which a variety of commercial products were derived.

Sign in / Sign up

Export Citation Format

Share Document