A lipidomics demonstration of the importance of single cell analysis

2015 ◽  
Vol 7 (9) ◽  
pp. 3668-3670 ◽  
Author(s):  
Mandy S. Phelps ◽  
Guido F. Verbeck
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Droplet Size ◽  
Lipid Droplet ◽  
Analytical Method ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Lipid Droplet Size

A novel single cell analytical method showing adipocyte heterogeneity dependent on lipid droplet size using nanomanipulation-coupled nanoelectrospray mass spectrometry.

Mass Spectrometry for Single-Cell Analysis

2020 ◽  
pp. 1-17
Author(s):  
Dinesh K. Patel ◽  
Sayan Deb Dutta ◽  
Ki-Taek Lim
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis

Single-cell analysis by ambient mass spectrometry

2017 ◽  
Vol 90 ◽  
pp. 14-26 ◽  
Author(s):  
Yunyun Yang ◽  
Yanying Huang ◽  
Junhui Wu ◽  
Ning Liu ◽  
Jiewei Deng ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Ambient Mass Spectrometry ◽  
Cell Analysis

Single Cell Analysis with Probe ESI-Mass Spectrometry: Detection of Metabolites at Cellular and Subcellular Levels

2014 ◽  
Vol 86 (8) ◽  
pp. 3809-3816 ◽  
Author(s):  
Xiaoyun Gong ◽  
Yaoyao Zhao ◽  
Shaoqing Cai ◽  
Shujie Fu ◽  
Chengdui Yang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Mass Spectrometry Detection ◽  
Esi Mass Spectrometry

Mass Spectrometry for Single-Cell Analysis

2021 ◽  
pp. 1033-1049
Author(s):  
Dinesh K. Patel ◽  
Sayan Deb Dutta ◽  
Ki-Taek Lim
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis

Mass spectrometry based detection of glutathione with sensitivity for single-cell analysis

2015 ◽  
Vol 29 (7) ◽  
pp. 681-689 ◽  
Author(s):  
Jia Yu ◽  
Chunyan Li ◽  
Shuijie Shen ◽  
Xiaoqiu Liu ◽  
Ying Peng ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis

scholarly journals Single-cell lipidomics with high structural specificity by mass spectrometry

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Zishuai Li ◽  
Simin Cheng ◽  
Qiaohong Lin ◽  
Wenbo Cao ◽  
Jing Yang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Cancer Cells ◽  
Single Cell Analysis ◽  
Single Cells ◽  
Mass Spectrometry Analysis ◽  
Human Lung Cancer ◽  
Cell Analysis ◽  
Structural Specificity ◽  
Spectrometry Analysis

AbstractSingle-cell analysis is critical to revealing cell-to-cell heterogeneity that would otherwise be lost in ensemble analysis. Detailed lipidome characterization for single cells is still far from mature, especially when considering the highly complex structural diversity of lipids and the limited sample amounts available from a single cell. We report the development of a general strategy enabling single-cell lipidomic analysis with high structural specificity. Cell fixation is applied to retain lipids in the cell during batch treatments prior to single-cell analysis. In addition to tandem mass spectrometry analysis revealing the class and fatty acyl-chain for lipids, batch photochemical derivatization and single-cell droplet treatment are performed to identify the C=C locations and sn-positions of lipids, respectively. Electro-migration combined with droplet-assisted electrospray ionization enables single-cell mass spectrometry analysis with easy operation but high efficiency in sample usage. Four subtypes of human breast cancer cells are correctly classified through quantitative analysis of lipid C=C location or sn-position isomers in ~160 cells. Most importantly, the single-cell deep lipidomics strategy successfully discriminates gefitinib-resistant cells from a population of wild-type human lung cancer cells (HCC827), highlighting its unique capability to promote precision medicine.

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