A potentiometric magnetic immunoassay for rapid detection of Salmonella typhimurium

2015 ◽  
Vol 7 (9) ◽  
pp. 4008-4011 ◽  
Author(s):  
Nádia F. D. Silva ◽  
Júlia M. C. S. Magalhães ◽  
M. Teresa Oliva-Teles ◽  
Cristina Delerue-Matos
Keyword(s):  
Salmonella Typhimurium ◽  
Rapid Detection ◽  
Limit Of Detection ◽  
Potentiometric Detection ◽  
Rapid Assay ◽  
Assay Time ◽  
Immunomagnetic Assay

We propose an immunomagnetic assay coupled to potentiometric detection suitable for sensitive (limit-of-detection 20 cells per mL) and rapid (assay time of 75 minutes) detection ofSalmonella typhimurium.

Application of Monoclonal Antibodies to Dulcitol 1-Phosphate Dehydrogenase for Rapid Detection of Salmonella

1995 ◽  
Vol 58 (8) ◽  
pp. 847-852 ◽  
Author(s):  
TAKAHISA MIYAMOTO ◽  
HUAIZE TIAN ◽  
KIYOSHI MATSUNO ◽  
RYOJI TAKATA ◽  
SHOJI HATANO
Keyword(s):  
Monoclonal Antibodies ◽  
Salmonella Typhimurium ◽  
Magnesium Chloride ◽  
Rapid Detection ◽  
Limit Of Detection ◽  
Sandwich Elisa ◽  
Enzyme Linked Immunosorbent Assay ◽  
The Novel ◽  
Inoculum Level ◽  
E Coli

Monoclonal antibodies raised against dulcitol 1-phosphate dehydrogenase of Salmonella typhimurium IFO 12529 were screened against 20 serotypes of Salmonella and 13 non-Salmonella bacteria. A sandwich-capture, enzyme-linked immunosorbent assay (sandwich ELISA) was developed for detection of Salmonella in food. The assay utilizes two monoclonal antibodies (DUI2 and DU28) which show no cross-reactions with non-Salmonella bacteria. The limit of detection of the sandwich ELISA was about 1 × 107 CPU/ml. After cultivation in a medium containing dulcitol at 37°C for 18 h followed by the sandwich ELISA. 1 CPU of Salmonella was detected. Although a high inoculum level of E. coli interfered with the detection of Salmonella, the interference was minimized by using a selective dulcitol-magnesium chloride-pyridinesulfonic acid medium for enrichment. The novel ELISA procedure detected Salmonella in chicken filtrates inoculated with 1.4 CPU/50 m1 and 1.3 × 107 CPU/50 ml of E. coli within 25 h.

scholarly journals Rapid immunoassays for detection of anabolic nortestosterone in dietary supplements

2013 ◽  
Vol 31 (No. 5) ◽  
pp. 514-519 ◽  
Author(s):  
B. Holubová ◽  
S. Göselová ◽  
L. Ševčíková ◽  
M. Vlach ◽  
M. Blažková ◽  
...  
Keyword(s):  
Dietary Supplements ◽  
Rapid Detection ◽  
Visual Detection ◽  
Limit Of Detection ◽  
Polyclonal Antibodies ◽  
Cross Reactivity ◽  
Rapid Analysis ◽  
Experimental Conditions ◽  
Immunochromatographic Strip ◽  
Strip Test

An enzyme immunoassay (ELISA) and an immunochromatographic strip were designed for a rapid detection of nortestosterone in dietary supplements. Two polyclonal antibodies and two types of nortestosterone-protein coating conjugates were tested to develop the most appropriate method. Under optimal experimental conditions, the most sensitive ELISA achieved the IC<sub>50 </sub>and the limit of detection values of 6.41 and 0.09 ng/ml, respectively. The assay specificity was tested measuring cross-reactivity of several steroids. The interference with the assay was negligible (&lt; 0.1%), except for cross-reactivity with another frequently abused steroid testosterone (23%). The optimised gold particle-based immunochromatographic strip provided in semi-quantitative test a visual detection limit of 1&nbsp;ng/ml. None of these methods showed the interference using a filtrate of the suspension of non-contaminated sample. After the validation for particular matrices, the ELISA and the strip test could be useful tools for a rapid analysis of nortestosterone in crude extracts of dietary supplements.

Optical Biosensor for Rapid Detection of Salmonella typhimurium Based on Porous Gold@Platinum Nanocatalysts and a 3D Fluidic Chip

ACS Sensors ◽  
2019 ◽  
Vol 5 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Lingyan Zheng ◽  
Gaozhe Cai ◽  
Wuzhen Qi ◽  
Siyuan Wang ◽  
Maohua Wang ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Rapid Detection ◽  
Optical Biosensor ◽  
Porous Gold ◽  
Gold Platinum

scholarly journals Handheld Enzymatic Luminescent Biosensor for Rapid Detection of Heavy Metals in Water Samples

Chemosensors ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 16 ◽  
Author(s):  
Kirill Lukyanenko ◽  
Ivan Denisov ◽  
Vladimir Sorokin ◽  
Anton Yakimov ◽  
Elena Esimbekova ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Rapid Detection ◽  
Limit Of Detection ◽  
Detection System ◽  
Test Procedure ◽  
Enzymatic Reaction ◽  
Water Quality Assessment ◽  
Micro Milling ◽  
Microfluidic Chips ◽  
Silicon Photomultiplier

Enzymatic luminescent systems are a promising tool for rapid detection of heavy metals ions for water quality assessment. Nevertheless, their widespread use is limited by the lack of test procedure automation and available sensitive handheld luminometers. Herein we describe integration of disposable microfluidic chips for bioluminescent enzyme-inhibition based assay with a handheld luminometer, which detection system is based on a thermally stabilized silicon photomultiplier (SiPM). Microfluidic chips were made of poly(methyl methacrylate) by micro-milling method and sealed using a solvent bonding technique. The composition of the bioluminescent system in microfluidic chip was optimized to achieve higher luminescence intensity and storage time. Results indicate that developed device provided comparable sensitivity with bench-scale PMT-based commercial luminometers. Limit of detection for copper (II) sulfate reached 2.5 mg/L for developed biosensor. Hereby we proved the concept of handheld enzymatic optical biosensors with disposable chips for bioassay. The proposed biosensor can be used as an early warning field-deployable system for rapid detection of heavy metals salts and other toxic chemicals, which affect bioluminescent signal of enzymatic reaction.

scholarly journals Rapid detection and differentiation of Salmonella species, Salmonella Typhimurium and Salmonella Enteritidis by multiplex quantitative PCR

PLoS ONE ◽  
2018 ◽  
Vol 13 (10) ◽  
pp. e0206316 ◽  
Author(s):  
Raymond Heymans ◽  
Amir Vila ◽  
Caroliene A. M. van Heerwaarden ◽  
Claudia C. C. Jansen ◽  
Greetje A. A. Castelijn ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Quantitative Pcr ◽  
Rapid Detection ◽  
Salmonella Enteritidis
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