Quantitative mass spectrometry imaging of small-molecule neurotransmitters in rat brain tissue sections using nanospray desorption electrospray ionization

The Analyst ◽  
2016 ◽  
Vol 141 (12) ◽  
pp. 3686-3695 ◽  
Author(s):  
Hilde-Marléne Bergman ◽  
Erik Lundin ◽  
Malin Andersson ◽  
Ingela Lanekoff
Keyword(s):  
Mass Spectrometry ◽  
Nervous System ◽  
Small Molecule ◽  
Mass Spectrometry Imaging ◽  
Quantitative Imaging ◽  
Desorption Electrospray Ionization ◽  
Quantitative Mass Spectrometry ◽  
Tissue Sections ◽  
System P ◽  
Rat Brain Tissue

Nano-DESI mass spectrometry imaging enables quantitative imaging of small-molecule neurotransmitters which are essential to the function of the nervous system.

scholarly journals Quantitative mass spectrometry imaging of glutathione in healthy and cancerous hen ovarian tissue sections by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

The Analyst ◽  
2018 ◽  
Vol 143 (3) ◽  
pp. 654-661 ◽  
Author(s):  
Milad Nazari ◽  
Mark T. Bokhart ◽  
Philip L. Loziuk ◽  
David C. Muddiman
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Mass Spectrometry Imaging ◽  
Ovarian Tissue ◽  
Desorption Electrospray Ionization ◽  
Laser Desorption ◽  
Quantitative Mass Spectrometry ◽  
Tissue Sections ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

IR-MALDESI quantitative mass spectrometry imaging of glutathione in healthy and cancerous hen ovarian tissues.

scholarly journals Polarity switching mass spectrometry imaging of healthy and cancerous hen ovarian tissue sections by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

The Analyst ◽  
2016 ◽  
Vol 141 (2) ◽  
pp. 595-605 ◽  
Author(s):  
Milad Nazari ◽  
David C. Muddiman
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Ovarian Tissue ◽  
Desorption Electrospray Ionization ◽  
Laser Desorption ◽  
Cancer Tissue ◽  
Ovarian Cancer Tissue ◽  
Tissue Sections ◽  
Polarity Switching ◽  
Matrix Assisted Laser

IR-MALDESI polarity switching mass spectrometry imaging reveals differences in lipid distribution in hen ovarian cancer tissue.

scholarly journals Desorption Electrospray Ionization then MALDI Mass Spectrometry Imaging of Lipid and Protein Distributions in Single Tissue Sections

2011 ◽  
Vol 83 (22) ◽  
pp. 8366-8371 ◽  
Author(s):  
Livia S. Eberlin ◽  
Xiaohui Liu ◽  
Christina R. Ferreira ◽  
Sandro Santagata ◽  
Nathalie Y.R. Agar ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Mass Spectrometry Imaging ◽  
Desorption Electrospray Ionization ◽  
Maldi Mass Spectrometry ◽  
Tissue Sections ◽  
Maldi Mass Spectrometry Imaging

scholarly journals Bedaquiline has potential for targeting tuberculosis reservoirs in the central nervous system

RSC Advances ◽  
2018 ◽  
Vol 8 (22) ◽  
pp. 11902-11907 ◽  
Author(s):  
Annapurna Pamreddy ◽  
Sooraj Baijnath ◽  
Tricia Naicker ◽  
Sphamandla Ntshangase ◽  
Sipho Mdanda ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Central Nervous System ◽  
Nervous System ◽  
Mass Spectrometry Imaging ◽  
Sprague Dawley ◽  
System P ◽  
Sprague Dawley Rats ◽  
The Central Nervous System ◽  
The Brain

Bedaquiline (BDQ) was administered to healthy Sprague-Dawley rats in order to determine its localisation in the brain using mass spectrometry imaging (MSI). This study shows that BDQ has the potential for targeting TB reservoirs in the CNS.

scholarly journals High Spatial Resolution Ambient Ionization Mass Spectrometry Imaging Using Microscopy Image Fusion Determines Tumor Margins

2019 ◽  
Author(s):  
Li-En Lin ◽  
Chih-Lin Chen ◽  
Ying-Chen Huang ◽  
Hsin-Hsiang Chung ◽  
Chiao-Wei Lin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Molecular Imaging ◽  
Image Fusion ◽  
Electrospray Ionization ◽  
Spatial Resolution ◽  
Mass Spectrometry Imaging ◽  
Desorption Electrospray Ionization ◽  
Ambient Ionization ◽  
Tumor Margins ◽  
Tissue Sections

AbstractMass spectrometry imaging (MSI) using ambient ionization technique enables a direct chemical investigation of biological samples with minimal sample pretreatment. However, detailed morphological information of the sample is often lost due to its limited spatial resolution. In this study, predictive high-resolution molecular imaging was produced by the fusion of ambient ionization MSI with optical microscopy of routine hematoxylin and eosin (H&E) staining produces. Specifically, desorption electrospray ionization (DESI) and nanospray desorption electrospray ionization (nanoDESI) mass spectrometry are employed to visualize lipid and protein species on mice tissue sections. The resulting molecular distributions obtained by ambient ionization MSI-microscopy fusion are verified with matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MSI and immunohistochemistry (IHC) staining. Label-free molecular imaging with 5-μm spatial resolution can be acquired using DESI and nanoDESI, whereas the typical spatial resolution of ambient ionization MSI is ~100 μm. In this regard, sharpened molecular histology of tissue sections is achieved, providing complementary references to the pathology. Such a multimodality integration enables the discovery of potential tumor biomarkers. After image fusion, more than a dozen of potential biomarkers that could be used to determine the tumor margins on a metastatic mouse lung tissue section and Luminal B breast tumor tissue section are identified.

scholarly journals Visualising Cholesterol in Brain by On-Tissue Derivatisation and Quantitative Mass Spectrometry Imaging

2020 ◽  
Author(s):  
Roberto Angelini ◽  
Eylan Yutuc ◽  
Mark F Wyatt ◽  
Jillian Newton ◽  
Fowzi Adam Yusuf ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Brain Structures ◽  
Brain Regions ◽  
Null Mouse ◽  
Brain Health ◽  
Quantitative Mass Spectrometry ◽  
Tissue Sections ◽  
Niemann Pick ◽  
The Brain

SummaryDespite being a critical molecule for neurobiology and brain health, mass spectrometry imaging (MSI) of cholesterol has been under reported compared to other lipids, due to the difficulty in ionising the sterol molecule. In the present work we have employed an on-tissue enzyme-assisted derivatisation strategy to improve detection of cholesterol in brain tissue sections. We report distribution and levels of cholesterol across specific brain structures of the mouse brain, in a model of Niemann-Pick type C1 (NPC1) disease, and during brain development. MSI revealed how cholesterol changes during development and that in the adult is highest in pons and medulla of the brain stem. Cholesterol was significantly reduced in the corpus callosum and other brain regions in the Npc1 null mouse, confirming hypomyelination at the molecular level. Our study demonstrates the potential of MSI to the study of sterols in neuroscience.

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