A unique dual recognition hairpin probe mediated fluorescence amplification method for sensitive detection of uracil-DNA glycosylase and endonuclease IV activities

The Analyst ◽  
2016 ◽  
Vol 141 (5) ◽  
pp. 1789-1795 ◽  
Author(s):  
Yushu Wu ◽  
Ping Yan ◽  
Xiaowen Xu ◽  
Wei Jiang
Keyword(s):  
Sensitive Detection ◽  
Dna Glycosylase ◽  
Uracil Dna Glycosylase ◽  
Hairpin Probe ◽  
Amplification Method ◽  
Recognition Probe ◽  
Fluorescence Amplification

A dual recognition probe mediated fluorescence amplification method was developed for sensitively and selectively detecting UDG and Endo IV activities.

A label-free and sensitive fluorescent method for the detection of uracil-DNA glycosylase activity

2015 ◽  
Vol 51 (5) ◽  
pp. 929-932 ◽  
Author(s):  
Jing Tao ◽  
Panshu Song ◽  
Yusuke Sato ◽  
Seiichi Nishizawa ◽  
Norio Teramae ◽  
...  
Keyword(s):  
Sensitive Detection ◽  
Dna Glycosylase ◽  
Label Free ◽  
Uracil Dna Glycosylase ◽  
Fluorescent Method

A label-free fluorescent method has been developed for sensitive detection of uracil-DNA glycosylase activity as well as UDG inhibitors.

Simple and sensitive detection of uracil–DNA glycosylase activity using dsDNA-templated copper nanoclusters as fluorescent probes

2016 ◽  
Vol 8 (22) ◽  
pp. 4319-4323 ◽  
Author(s):  
Manman Cao ◽  
Yan Jin ◽  
Baoxin Li
Keyword(s):  
Fluorescent Probes ◽  
Sensitive Detection ◽  
Dna Glycosylase ◽  
Copper Nanoclusters ◽  
Uracil Dna Glycosylase

DNA-templated copper nanoclusters were used as a fluorescent nano-dye for the uracil–DNA glycosylase (UDG) assay. The proposed method is simple and sensitive.

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