Multiplex microfluidic system integrating sequential operations of microalgal lipid production

The Analyst ◽  
2016 ◽  
Vol 141 (4) ◽  
pp. 1218-1225 ◽  
Author(s):  
Ho Seok Kwak ◽  
Jaoon Young Hwan Kim ◽  
Sang Cheol Na ◽  
Noo Li Jeon ◽  
Sang Jun Sim
Keyword(s):  
Sample Preparation ◽  
Lipid Production ◽  
Microfluidic System ◽  
Lipid Sample ◽  
Micropillar Arrays

We developed a PDMS-based multiplex microfluidic system with eight chambers and micropillar arrays to expedite multiple steps for lipid sample preparation from different microalgal strains.

An Integrated Micro/Nanofluidic System for Processing of Protein Samples

2011 ◽  
Author(s):  
Khalid Anwar ◽  
Taeheon Han ◽  
Sun Min Kim
Keyword(s):  
Sample Preparation ◽  
Electrical Breakdown ◽  
Protein Analysis ◽  
Microfluidic System ◽  
Pdms Membrane ◽  
Electrical Shock ◽  
Separation Column ◽  
Nanofluidic System

In this study, an integrated micro/nanofluidic system for protein analysis was presented. The device is comprised of a micromixer and a preconcentrator with a separation column. The integrated micromixer based on unbalanced split and cross collision of fluid streams is passive and planar, which is easy to fabricate and integrate to the microfluidic system. The preconcentrator has nanochannels formed by the electrical breakdown of polydimethylsiloxane (PDMS) membrane using a high electrical shock, without any nano-lithographic process. Micromixer and preconcentrator were used for sample preparation (tagging of protein for detection) and concentration of protein, consecutively. Proteins were electrokinetically trapped near the junction of micro/nanochannels.

Parallel sample preparation of proteins, from crude samples to crystals ready for MALDI-MS, in an integrated microfluidic system

2010 ◽  
Vol 878 (28) ◽  
pp. 2803-2810 ◽  
Author(s):  
Tran Thi Thuy ◽  
Mats Inganäs ◽  
Gunnar Ekstrand ◽  
Gunnar Thorsén
Keyword(s):  
Sample Preparation ◽  
Microfluidic System ◽  
Maldi Ms ◽  
Parallel Sample

Miniaturized Blood Sampling System with Integrated Sample Preparation

2021 ◽  
Vol 1016 ◽  
pp. 1280-1284
Author(s):  
Susanne Schlegel ◽  
Erik Krumnow ◽  
Andrea Böhme ◽  
Marilena Minieri ◽  
Andreas H. Foitzik
Keyword(s):  
Sample Preparation ◽  
First Generation ◽  
Microfluidic System ◽  
Sampling Procedure ◽  
Flow Time ◽  
Blood Sampling ◽  
Micro Milling ◽  
Sampling System ◽  
New Device ◽  
Number Of Patients

Blood sampling as well as sample preparation are time consuming and requires a strict procedure, which is generally performed by medical trained personal. Not carrying out the procedure correctly could result in an infection of the patient or contamination of the sample itself. These limitations should be especially considered in case of pandemic outbreaks. In order to handle such a high number of patients a novel sample preparation system paired with modern blood sampling procedure is necessary. For this reason, a new device for blood sampling and preparation is designed containing an integrated microfluidic system. The fabrication is carried out by utilizing micro moulding of PDMS as well as micro milling. A first set of initial experiments as part of a first-generation study shows promising results. However, further steps of optimisation considering flow time and preparation cycle are part of a second-generation study.

scholarly journals An Easily Accessible Microfluidic Chip for High-Throughput Microalgae Screening for Biofuel Production

Energies ◽  
2021 ◽  
Vol 14 (7) ◽  
pp. 1817
Author(s):  
Shubhanvit Mishra ◽  
Yi-Ju Liu ◽  
Chi-Shuo Chen ◽  
Da-Jeng Yao
Keyword(s):  
Lipid Content ◽  
High Efficiency ◽  
Lipid Production ◽  
Microfluidic System ◽  
Green Energy ◽  
Numerical Control ◽  
Biofuel Production ◽  
Micro Milling ◽  
Hanging Drop ◽  
Pump System

Microalgae are important green energy resources. With high efficiency in fixing carbon dioxide, microalgae are broadly applied for biofuel production. Integrating various cultivation parameters, we applied ultraviolet (UV) mutagenesis, one of the most common approaches, to induce genomic mutation in microalgae and thus enhance the production of lipid content, but the screening process is convoluted and labor-intensive. In this study, we aimed to develop an accessible microfluidic platform to optimize the biofuel production of microalgae. Instead of traditional lithography, we designed hanging-drop microfluidic chips that were fabricated using a cheap computer numerical control (CNC) micro-milling technique. On each chip, we cultured in parallel Botryococcus braunii, one of the most common freshwater microalgae for biofuel production, in sets of ten separated hanging drops (~30 µL each); we monitored their growth in each individual drop for more than 14 days. To optimize the culturing conditions, using drops of varied diameter, we first identified the influence of cell density on algae growth and lipid production. After introducing UV-induced random mutations, we quantified the lipid content of the microalgae in situ; the optimized UV-C dosage was determined accordingly. In comparison with wild-type B. braunii, the results showed increased biomass growth (137%) and lipid content (149%) of the microalgae mutated with the desired UV process. Moreover, we showed a capacity to modulate the illumination on an addressed chip area. In summary, without using an external pump system, we developed a hanging-drop microfluidic system for long-term microalgae culturing, which can be easily operated using laboratory pipettes. This microfluidic system is expected to facilitate microalgae mutation breeding, and to be applied for algae cultivation optimization.

Pollutant Identification by Selected Area Electron Diffraction

1974 ◽  
Vol 32 ◽  
pp. 532-533
Author(s):  
R. E. Ferrell ◽  
G. G. Paulson ◽  
C. W. Walker
Keyword(s):  
Thermal Treatment ◽  
Electron Diffraction ◽  
Sample Preparation ◽  
Crystal Structures ◽  
Water Samples ◽  
Environmental Samples ◽  
Short Review ◽  
Selected Area Electron Diffraction ◽  
Multiple Component

Selected area electron diffraction (SAD) has been used successfully to determine crystal structures, identify traces of minerals in rocks, and characterize the phases formed during thermal treatment of micron-sized particles. There is an increased interest in the method because it has the potential capability of identifying micron-sized pollutants in air and water samples. This paper is a short review of the theory behind SAD and a discussion of the sample preparation employed for the analysis of multiple component environmental samples.

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