FTIR imaging of the 3D extracellular matrix used to grow colonies of breast cancer cell lines

The Analyst ◽  
2016 ◽  
Vol 141 (2) ◽  
pp. 620-629 ◽  
Author(s):  
Margarita Smolina ◽  
Erik Goormaghtigh
Keyword(s):  
Breast Cancer ◽  
Extracellular Matrix ◽  
Basement Membrane ◽  
Cell Lines ◽  
Breast Cancer Cell ◽  
Cell Cultures ◽  
Infrared Imaging ◽  
Three Dimensional ◽  
Breast Cancer Cell Lines ◽  
Dimensional Cell

Infrared imaging was applied to investigate a reconstituted basement membrane, known as Matrigel, in three-dimensional cell cultures.

Three-dimensional extracellular matrix culture models of EGFR signalling and drug response

2007 ◽  
Vol 35 (4) ◽  
pp. 665-668 ◽  
Author(s):  
P.A. Kenny
Keyword(s):  
Breast Cancer ◽  
Extracellular Matrix ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Drug Response ◽  
Three Dimensional ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Culture Models

Three-dimensional extracellular matrix culture, on substrata such as Matrigel, restores many aspects of the differentiated state to non-malignant cells from a variety of tissues. We have adapted these techniques to study EGFR (epidermal growth factor receptor) signalling and drug response in breast cancer cell lines. EGFR-dependent breast cancer cell lines undergo a striking reversion of the malignant phenotype upon treatment with inhibitors targeting the receptor, or downstream signalling intermediates such as mitogen-activated protein kinase and PI3K (phosphoinositide 3-kinase). Using this approach, we have recently reported that EGFR signalling in breast cancer can be effectively inhibited by blocking the activity of a key protease, TACE [TNFα (tumour necrosis factor α)-converting enzyme], which regulates the bioavailability of EGFR ligands. These results suggest a new way to target EGFR signalling in tumours of the breast and other epithelial tissues and underline the value of three-dimensional extracellular matrix culture models for exploring cancer-relevant signalling processes ex vivo.

scholarly journals The Efficiency of Gene Electrotransfer in Breast-Cancer Cell Lines Cultured on a Novel Collagen-Free 3D Scaffold

Cancers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 1043 ◽  
Author(s):  
Elisabetta Sieni ◽  
Monica Dettin ◽  
Mariangela De Robertis ◽  
Bianca Bazzolo ◽  
Maria Teresa Conconi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Breast Cancer Cell ◽  
Cell Cultures ◽  
Transfection Efficiency ◽  
Breast Cancer Cell Lines ◽  
The Novel ◽  
3D Scaffold

Gene Electro-Transfer (GET) is a powerful method of DNA delivery with great potential for medical applications. Although GET has been extensively studied in vitro and in vivo, the optimal parameters remain controversial. 2D cell cultures have been widely used to investigate GET protocols, but have intrinsic limitations, whereas 3D cultures may represent a more reliable model thanks to the capacity of reproducing the tumor architecture. Here we applied two GET protocols, using a plate or linear electrode, on 3D-cultured HCC1954 and MDA-MB231 breast cancer cell lines grown on a novel collagen-free 3D scaffold and compared results with conventional 2D cultures. To evaluate the electrotransfer efficiency, we used the plasmid pEGFP-C3 encoding the enhanced green fluorescent protein (EGFP) reporter gene. The novel 3D scaffold promoted extracellular matrix deposition, which particularly influences cell behavior in both in vitro cell cultures and in vivo tumor tissue. While the transfection efficiency was similar in the 2D-cultures, we observed significant differences in the 3D-model. The transfection efficiency in the 3D vs 2D model was 44% versus 15% (p < 0.01) and 24% versus 17% (p < 0.01) in HCC1954 and MDA-MB231 cell cultures, respectively. These findings suggest that the novel 3D scaffold allows reproducing, at least partially, the peculiar morphology of the original tumor tissues, thus allowing us to detect meaningful differences between the two cell lines. Following GET with plate electrodes, cell viability was higher in 3D-cultured HCC1954 (66%) and MDA-MB231 (96%) cell lines compared to their 2D counterpart (53% and 63%, respectively, p < 0.001). Based on these results, we propose the novel 3D scaffold as a reliable support for the preparation of cell cultures in GET studies. It may increase the reliability of in vitro assays and allow the optimization of GET parameters of in vivo protocols.

scholarly journals The morphologies of breast cancer cell lines in three-dimensional assays correlate with their profiles of gene expression

2007 ◽  
Vol 1 (1) ◽  
pp. 84-96 ◽  
Author(s):  
Paraic A. Kenny ◽  
Genee Y. Lee ◽  
Connie A. Myers ◽  
Richard M. Neve ◽  
Jeremy R. Semeiks ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Three Dimensional ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines
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