Deformability measurement of red blood cells using a microfluidic channel array and an air cavity in a driving syringe with high throughput and precise detection of subpopulations

The Analyst ◽  
2016 ◽  
Vol 141 (1) ◽  
pp. 319-330 ◽  
Author(s):  
Yang Jun Kang ◽  
Young-Ran Ha ◽  
Sang-Joon Lee
Keyword(s):  
Red Blood Cells ◽  
High Throughput ◽  
Blood Cells ◽  
Microfluidic Channel ◽  
New Method ◽  
Air Cavity ◽  
Blood Samples ◽  
Hematological Disorders

We propose a new method to measure deformability of blood samples containing hematological disorders with high throughput and precise detection of subpopulations.

scholarly journals Diagnoses of Blood Disorder in Different Animal Species Depending on Counting Methods in Blood Cell Images

2018 ◽  
Vol 7 (4.36) ◽  
pp. 660
Author(s):  
Kawther H. Al-khafaji ◽  
Athraa H. Al-khafaji
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Cell Shape ◽  
Animal Species ◽  
Vision System ◽  
Blood Samples ◽  
Hematological Disorders ◽  
Blood Disorder ◽  
The Difference

Counting of red blood cells (RBCs) in microscope blood cell images, can give the pathologists valuable information regarding various hematological disorders, like anemia, leukemia,....etc. in several animal species, in this paper, an automated vision system has been developed which is capable of counting of red blood cells, in blood samples by applying different algorithms, based on red blood cellshape, the difference in the red blood cell shape of animal species make it difficult to use a one algorithm, therefore, for each animal species used specific algorithm which was capable of counting of RBCs effectively. 

Sonoporation enables high-throughput loading of trehalose into red blood cells

Cryobiology ◽  
2020 ◽  
Author(s):  
Brett R. Janis ◽  
Mariah C. Priddy ◽  
Meghan R. Otto ◽  
Jonathan A. Kopechek ◽  
Michael A. Menze
Keyword(s):  
Red Blood Cells ◽  
High Throughput ◽  
Blood Cells

scholarly journals High-throughput assessment of mechanical properties of stem cell derived red blood cells, toward cellular downstream processing

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ewa Guzniczak ◽  
Maryam Mohammad Zadeh ◽  
Fiona Dempsey ◽  
Melanie Jimenez ◽  
Henry Bock ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Stem Cell ◽  
Red Blood Cells ◽  
High Throughput ◽  
Blood Cells ◽  
Downstream Processing

scholarly journals Acetylsalicylic acid and morphology of red blood cells

2010 ◽  
Vol 53 (3) ◽  
pp. 575-582 ◽  
Author(s):  
Jacques Natan Grinapel Frydman ◽  
Adenilson de Souza da Fonseca ◽  
Vanessa Câmara da Rocha ◽  
Monica Oliveira Benarroz ◽  
Gabrielle de Souza Rocha ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Control Group ◽  
Blood Samples ◽  
Morphological Alterations ◽  
Blood Smears ◽  
In Vivo Treatment ◽  
Microscopy Data

This work evaluated the effect of in vitro and in vivo treatment with ASA on the morphology of the red blood cells. Blood samples or Wistar rats were treated with ASA for one hour. Blood samples or animals treated with saline were used as control group. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of red blood cells were evaluated under optical microscopy. Data showed that the in vitro treatment for one hour with ASA at higher dose used significantly (p<0.05) modified the perimeter/area ratio of the red blood cells. No morphological alterations were obtained with the in vivo treatment. ASA use at highest doses could interfere on shape of red blood cells.

scholarly journals High‐throughput adaptation for the quantitation of total folate in human red blood cells by LC‐MS/MS

2006 ◽  
Vol 20 (4) ◽  
Author(s):  
Janel Elizabeth Owens ◽  
Dirk M. Holstege ◽  
Andrew J. Clifford
Keyword(s):  
Red Blood Cells ◽  
High Throughput ◽  
Blood Cells ◽  
Human Red Blood Cells

Blood Volumes of Dairy Calves Comparing Cr51-Tagged Red Blood Cells and T-1824 Plasma Dilution Methods

1958 ◽  
Vol 193 (2) ◽  
pp. 244-248 ◽  
Author(s):  
Perry Ruth Stahl ◽  
Homer E. Dale
Keyword(s):  
Body Weight ◽  
Red Blood Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Dairy Calves ◽  
Computation Method ◽  
Blood Samples ◽  
Whole Blood Samples ◽  
Blood Volumes

In a repeated study on 17 dairy calves, T-1824 dye plasma dilution showed significantly higher blood volumes than were found by any other technique or computation method using Cr51-tagged red blood cells. Five blood samples taken at 20-minute intervals after injection showed consistent decrease in radioactivity count from the first to the last sample, indicating greater accuracy in radioactivity dilution regressed to zero time figures than in average counts of several postinjection samples. In vitro studies suggest a loss of Cr51 from red blood cells to plasma after saline washings are Cr-free. Percentage blood volumes computed from whole blood samples of calves injected with Cr51-tagged red blood cells decreased in a straight line relationship with increase of body weight. Percentage plasma and whole blood volumes estimated with the T-1824 dye technique decreased regularly with body weight increase until a second determination was made when there was a rapid rise nearly to the level of the smallest calves, followed by another regular decrease with increase in weight. It is suggested that repeated dye injections do not always measure the same space. Regressed values of five whole blood samples taken at 20-minute intervals after injection of Cr51 tagged red blood cells gave more consistent blood volume determinations than either the weighed red cells or the plasma dye dilutions of the same samples.

A new method for isolation of purified genomic DNA from haemosporidian parasites inhabiting nucleated red blood cells

2013 ◽  
Vol 133 (3) ◽  
pp. 275-280 ◽  
Author(s):  
Vaidas Palinauskas ◽  
Asta Križanauskienė ◽  
Tatjana A. Iezhova ◽  
Casimir V. Bolshakov ◽  
Jane Jönsson ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Genomic Dna ◽  
New Method ◽  
Nucleated Red Blood Cells ◽  
Haemosporidian Parasites

Standardization of anti-human globulin sera for phenotyping red blood cells using a new method

Vox Sanguinis ◽  
2002 ◽  
Vol 82 (1) ◽  
pp. 52-52
Author(s):  
F. Llopis ◽  
F. Carbonell-Uberos ◽  
M. C. Montero ◽  
I. Terrón
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
New Method

A NEW METHOD OF COUNTING RED BLOOD CELLS

JAMA ◽  
1930 ◽  
Vol 94 (20) ◽  
pp. 1573
Author(s):  
William G. Exton
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
New Method
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