scholarly journals Novel intramolecular photoinduced electron transfer-based probe for the Human Ether-a-go-go-Related Gene (hERG) potassium channel

The Analyst ◽  
2015 ◽  
Vol 140 (24) ◽  
pp. 8101-8108 ◽  
Author(s):  
Zhenzhen Liu ◽  
Yubin Zhou ◽  
Lupei Du ◽  
Minyong Li
Keyword(s):  
Electron Transfer ◽  
Potassium Channel ◽  
Photoinduced Electron Transfer ◽  
Herg Channel ◽  
Good Activity ◽  
Related Gene ◽  
Careful Evaluation ◽  
Promising Application

A novel conformation-mediated intramolecular photoinduced electron transfer fluorogenic system based-on naphthalimide fluorophore was established for hERG potassium channel herein. After careful evaluation, probe N4 and N6 showed good activity and may have a promising application in hERG channel imaging and drug cardiotoxicity evaluation.

How to Fluorescently Label the Potassium Channel: A Case in hERG

2020 ◽  
Vol 27 (18) ◽  
pp. 3046-3054
Author(s):  
Xiaomeng Zhang ◽  
Beilei Wang ◽  
Zhenzhen Liu ◽  
Yubin Zhou ◽  
Lupei Du
Keyword(s):  
Potassium Channel ◽  
Herg Channel ◽  
Pore Channel ◽  
Design Rationale ◽  
Related Gene ◽  
Open Conformation ◽  
Cardiac Action ◽  
Qt Syndrome ◽  
Herg Channels ◽  
Action Potential Repolarization

hERG (Human ether-a-go-go-related gene) potassium channel, which plays an essential role in cardiac action potential repolarization, is responsible for inherited and druginduced long QT syndrome. Recently, the Cryo-EM structure capturing the open conformation of hERG channel was determined, thus pushing the study on hERG channel at 3.8 Å resolution. This report focuses primarily on summarizing the design rationale and application of several fluorescent probes that target hERG channels, which enables dynamic and real-time monitoring of potassium pore channel affinity to further advance the understanding of the channels.

scholarly journals NBD-Based Environment-Sensitive Fluorescent Probes for the Human Ether-a-Go-Go–Related Gene Potassium Channel

2021 ◽  
Vol 8 ◽  
Author(s):  
Qi Li ◽  
Lijuan Chai ◽  
Gaopan Dong ◽  
Xiaomeng Zhang ◽  
Lupei Du
Keyword(s):  
Fluorescent Proteins ◽  
Fluorescent Labeling ◽  
Herg Channel ◽  
Screening System ◽  
Related Gene ◽  
Careful Evaluation ◽  
Economical Method ◽  
High Affinity ◽  
Herg Channels

Three environment-sensitive probes were developed for the hERG channel based on the nitrobenzoxadiazole fluorophore herein. After careful evaluation, probes M1 and M3 were found to have a high affinity for imaging the hERG channel in the cell-based experiment. Compared with other fluorescent labeling technologies (such as fluorescent proteins), these probes afford a convenient and economical method to determine hERG channel in vitro and in cellulo. Therefore, these probes are expected to be applicable for usage in physiological and pathological studies of hERG channels and have the potential to establish a screening system for hERG channels.

Computationally Assisted Design of High Signal-to-Noise Photoinduced Electron Transfer-Based Voltage-Sensitive Dyes

2020 ◽  
Author(s):  
Rishikesh Kulkarni ◽  
Anneliese Gest ◽  
Chun Kei Lam ◽  
Benjamin Raliski ◽  
Feroz James ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Dft Calculations ◽  
Photoinduced Electron Transfer ◽  
Density Functional ◽  
Embryonic Stem ◽  
High Sensitivity ◽  
Md Simulations ◽  
High Signal ◽  
Signal To Noise ◽  
Green Fluorescent

<p>High signal-to-noise optical voltage indicators will enable simultaneous interrogation of membrane potential in large ensembles of neurons. However, design principles for voltage sensors with high sensitivity and brightness remain elusive, limiting the applicability of voltage imaging. In this paper, we use molecular dynamics (MD) simulations and density functional theory (DFT) calculations to guide the design of a bright and sensitive green-fluorescent voltage-sensitive fluorophore, or VoltageFluor (VF dye), that uses photoinduced electron transfer (PeT) as a voltage-sensing mechanism. MD simulations predict an 11% increase in sensitivity due to membrane orientation, while DFT calculations predict an increase in fluorescence quantum yield, but a decrease in sensitivity due to a decrease in rate of PeT. We confirm these predictions by synthesizing a new VF dye and demonstrating that it displays the expected improvements by doubling the brightness and retaining similar sensitivity to prior VF dyes. Combining theoretical predictions and experimental validation has resulted in the synthesis of the highest signal-to-noise green VF dye to date. We use this new voltage indicator to monitor the electrophysiological maturation of human embryonic stem cell-derived medium spiny neurons. </p>

Photoinduced electron transfer in mechanically interlocked suit[3]ane systems

2021 ◽  
Author(s):  
Anton J Stasyuk ◽  
Olga A. Stasyuk ◽  
Miquel Solà ◽  
Alexander Voityuk
Keyword(s):  
Electron Transfer ◽  
Photoinduced Electron Transfer ◽  
New Class ◽  
Transfer Processes ◽  
Two Component ◽  
Electron Transfer Processes

Suitanes, a new class of two-component mechanically interlocked systems, have recently been developed. In this work, we report a detailed study of photoinduced electron transfer processes in the suit[3]anes consisting...

scholarly journals A quantum optics approach to photoinduced electron transfer in cavities

2021 ◽  
Vol 154 (5) ◽  
pp. 054104
Author(s):  
D. Wellnitz ◽  
G. Pupillo ◽  
J. Schachenmayer
Keyword(s):  
Electron Transfer ◽  
Quantum Optics ◽  
Photoinduced Electron Transfer
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