Adsorption between TC-stabilized AuNPs and the phosphate group: application of the PTP1B activity assay

The Analyst ◽  
2015 ◽  
Vol 140 (23) ◽  
pp. 8017-8022 ◽  
Author(s):  
Jun Lv ◽  
Xiaonan Wang ◽  
Yuanyuan Zhang ◽  
Defeng Li ◽  
Juan Zhang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Magnetic Nanoparticles ◽  
Colorimetric Method ◽  
Phosphate Group ◽  
Activity Assay ◽  
Phosphate Groups

Based on the adsorption between tetracycline (TC) and phosphate groups, a general colorimetric method is explored in this work by using TC-stabilized gold nanoparticles (TC/AuNPs) and 4-aminophenyl phosphate-functionalized Fe3O4 magnetic nanoparticles (APP/MNPs).

A colorimetric method for the determination of xanthine based on the aggregation of gold nanoparticles

2014 ◽  
Vol 182 (1-2) ◽  
pp. 395-400 ◽  
Author(s):  
Wendan Pu ◽  
Huawen Zhao ◽  
Liping Wu ◽  
Xianying Zhao
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Method

A Simple Colorimetric Method for Naked-Eye Detection of Circulating Cell-Free DNA Using Unlabelled Gold Nanoparticles

2018 ◽  
Vol 3 (41) ◽  
pp. 11541-11551 ◽  
Author(s):  
Shweta Khanna ◽  
Prasanta Padhan ◽  
Sourav Das ◽  
Kumar Sagar Jaiswal ◽  
Archana Tripathy ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Method ◽  
Eye Detection ◽  
Cell Free Dna ◽  
Naked Eye ◽  
Free Dna ◽  
Naked Eye Detection ◽  
Circulating Cell Free Dna

scholarly journals A sensitive, accurate, and high-throughput gluco-oligosaccharide oxidase based HRP colorimetric method for lytic polysaccharide monooxygenase activity assay

2021 ◽  
Author(s):  
Gang Liu
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Direct Method ◽  
Total Concentration ◽  
Colorimetric Method ◽  
Industrial Applications ◽  
Assay Method ◽  
Activity Assay ◽  
Lytic Polysaccharide Monooxygenase ◽  
Monooxygenase Activity

Abstract Background The AA9 (auxiliary activities) family of lytic polysaccharide monooxygenases (AA9 LPMOs) are ubiquitous and diverse group of enzymes amongst the fungal kingdom. They catalyze the oxidative cleavage of glycosidic bonds in lignocellulose and exhibit great potential for secondary biorefinery applications. Screening of AA9 LPMOs for desirable properties is crucial for biorefinery industrial applications. However, robust, high-throughput and direct method for AA9 LPMO activity assay, which is prerequisite for screening of LPMOs with excellent properties, is still lacking. Here, we have described a gluco-oligosaccharide oxidase (GOOX) based horseradish peroxidase (HRP) colorimetric method for AA9 LPMO activity assay. Results We cloned and expressed a GOOX gene from Sarocladium strictum in Trichoderma reesei, purified the recombinant SsGOOX, validated its properties, and set up a SsGOOX based HRP colorimetric method for cellobiose concentration assay. Then we expressed two AA9 LPMOs from Thielavia terrestris, TtAA9F and TtAA9G in T. reesei, purified the recombinant proteins, and analyzed their product profiles and regioselectivity towards phosphoric acid swollen cellulose (PASC). TtAA9F was characterized as a C1 type (class 1) LPMO, while TtAA9G was characterized as a C4 type (class 2) LPMO. Finally, the SsGOOX based HRP colorimetric method was used to quantify the total concentration of reducing lytic products from LPMO reaction, and consequently, the activities of both C1 and C4 types of LPMOs were analyzed. These LPMOs could be effectively analyzed with limits of detection (LoDs) lower than 30 nmol/L, and standard curves between A515 and LPMO concentrations with determination coefficients greater than 0.994 were obtained. Conclusions A novel, sensitive and accurate assay method that directly targets the main activity of both C1 and C4 type of AA9 LPMOs was established. This method is easy to use and could be performed on a microtiter plate ready for high-throughput screening of AA9 LPMOs with high properties.

The first 3′:5′-cyclic nucleotide–amino acid complex:L-His–cIMP

2012 ◽  
Vol 68 (8) ◽  
pp. o311-o316 ◽  
Author(s):  
Katarzyna Ślepokura
Keyword(s):  
Crystal Structure ◽  
Amino Acid ◽  
Cyclic Nucleotide ◽  
Phosphate Group ◽  
Specific Amino Acid ◽  
Π Stacking ◽  
Cyclic Phosphate ◽  
Nucleotide Recognition ◽  
Group Play ◽  
Phosphate Groups

In the crystal structure of the L-His–cIMP complex,i.e.L-histidinium inosine 3′:5′-cyclic phosphate [systematic name: 5-(2-amino-2-carboxyethyl)-1H-imidazol-3-ium 7-hydroxy-2-oxo-6-(6-oxo-6,9-dihydro-1H-purin-9-yl)-4a,6,7,7a-tetrahydro-4H-1,3,5,2λ5-furo[3,2-d][1,3,2λ5]dioxaphosphinin-2-olate], C6H10N3O2+·C10H10N4O7P−, the Hoogsteen edge of the hypoxanthine (Hyp) base of cIMP and the Hyp face are engaged in specific amino acid–nucleotide (His...cIMP) recognition,i.e.by abutting edge-to-edge and by π–π stacking, respectively. The Watson–Crick edge of Hyp and the cIMP phosphate group play a role in nonspecific His...cIMP contacts. The interactions between the cIMP anions (anti/C3′–endo/trans–gauche/chair conformers) are realized mainly between riboses and phosphate groups. The results for this L-His–cIMP complex, compared with those for the previously reported solvated L-His–IMP crystal structure, indicate a different nature of amino acid–nucleotide recognition and interactions upon the 3′:5′-cyclization of the nucleotide phosphate group.

The Position of Phosphate Groups in the Phosphonomannan of Hansenula capsulata, as Determined by Carbon-13 Magnetic Resonance Spectroscopy

1973 ◽  
Vol 51 (13) ◽  
pp. 2105-2109 ◽  
Author(s):  
Philip A. J. Gorin
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Spectral Data ◽  
Resonance Spectrum ◽  
Phosphate Group ◽  
Resonance Spectroscopy ◽  
Previous Assignment ◽  
Phosphate Groups

The carbon-13 magnetic resonance spectrum of a phosphate of 2-O-β-D-mannopyranosyl-α,β-D-mannose was compared with that of the unphosphorylated disaccharide. The positions and number of the signals of C-5's which are coupled to phosphorus-31, together with other spectral data show that the phosphate group is on C-6 of the reducing end-unit (2, Fig. 1). This differs from a previous assignment (1), thus necessitating a revision (4) to the structure 3 proposed for the parent Hansenula capsulata phosphonomannan.

A smart and rapid colorimetric method for the detection of codeine sulphate, using unmodified gold nanoprobe

RSC Advances ◽  
2014 ◽  
Vol 4 (92) ◽  
pp. 50443-50448 ◽  
Author(s):  
Anand Lodha ◽  
Alok Pandya ◽  
Pinkesh G. Sutariya ◽  
Shobhana K. Menon
Keyword(s):  
Gold Nanoparticles ◽  
Electrochemical Properties ◽  
Colorimetric Method ◽  
High Sensitivity ◽  
Toxicological Screening ◽  
Spot Detection ◽  
Gold Nanoprobe

Herein, we reported unique optical and electrochemical properties of citrate-stabilized gold nanoparticles (AuNPs) as a probe for smartphone-assisted, on-spot detection of codeine sulphate in toxicological screening with high sensitivity (0.9 μM).

A novel and environmentally friendly colorimetric method for detection of cystine in human urine using unmodified gold nanoparticles

RSC Advances ◽  
2014 ◽  
Vol 4 (52) ◽  
pp. 27297-27300 ◽  
Author(s):  
Li-Qiang Lu ◽  
Qian Gao ◽  
Chi Song ◽  
Xi-Ke Tian ◽  
An-Wu Xu
Keyword(s):  
Gold Nanoparticles ◽  
Human Urine ◽  
Colorimetric Detection ◽  
Colorimetric Method ◽  
Environmentally Friendly

A novel and environmentally friendly strategy based on unmodified AuNPs was developed for the colorimetric detection of cystine in human urine.

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