scholarly journals Emulsion technologies for multicellular tumour spheroid radiation assays

The Analyst ◽  
2016 ◽  
Vol 141 (1) ◽  
pp. 100-110 ◽  
Author(s):  
Kay S. McMillan ◽  
Anthony G. McCluskey ◽  
Annette Sorensen ◽  
Marie Boyd ◽  
Michele Zagnoni
Keyword(s):  
Cytotoxic Effect ◽  
Tumour Growth ◽  
Tumour Spheroid ◽  
Multicellular Tumour Spheroid

Emulsion technology is used to assess the cytotoxic effect of radiation on spheroids by mimicking tumour growth and tumour quiescence.

scholarly journals Evaluation of the PSMA-Binding Ligand 212Pb-NG001 in Multicellular Tumour Spheroid and Mouse Models of Prostate Cancer

2021 ◽  
Vol 22 (9) ◽  
pp. 4815
Author(s):  
Vilde Yuli Stenberg ◽  
Roy Hartvig Larsen ◽  
Li-Wei Ma ◽  
Qian Peng ◽  
Petras Juzenas ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mouse Models ◽  
Cytotoxic Effect ◽  
Therapeutic Potential ◽  
Therapeutic Effects ◽  
Radiation Toxicity ◽  
Castration Resistant Prostate Cancer ◽  
Tumour Spheroid ◽  
Multicellular Tumour Spheroid

Radioligand therapy targeting the prostate-specific membrane antigen (PSMA) is rapidly evolving as a promising treatment for metastatic castration-resistant prostate cancer. The PSMA-targeting ligand p-SCN-Bn-TCMC-PSMA (NG001) labelled with 212Pb efficiently targets PSMA-positive cells in vitro and in vivo. The aim of this preclinical study was to evaluate the therapeutic potential of 212Pb-NG001 in multicellular tumour spheroid and mouse models of prostate cancer. The cytotoxic effect of 212Pb-NG001 was tested in human prostate C4-2 spheroids. Biodistribution at various time points and therapeutic effects of different activities of the radioligand were investigated in male athymic nude mice bearing C4-2 tumours, while long-term toxicity was studied in immunocompetent BALB/c mice. The radioligand induced a selective cytotoxic effect in spheroids at activity concentrations of 3–10 kBq/mL. In mice, the radioligand accumulated rapidly in tumours and was retained over 24 h, while it rapidly cleared from nontargeted tissues. Treatment with 0.25, 0.30 or 0.40 MBq of 212Pb-NG001 significantly inhibited tumour growth and improved median survival with therapeutic indexes of 1.5, 2.3 and 2.7, respectively. In BALB/c mice, no signs of long-term radiation toxicity were observed at activities of 0.05 and 0.33 MBq. The obtained results warrant clinical studies to evaluate the biodistribution, therapeutic efficacy and toxicity of 212Pb-NG001.

Microdevice arrays of high aspect ratio poly(dimethylsiloxane) pillars for the investigation of multicellular tumour spheroid mechanical properties

Lab on a Chip ◽  
2014 ◽  
Vol 14 (13) ◽  
pp. 2344-2353 ◽  
Author(s):  
Laurène Aoun ◽  
Pierre Weiss ◽  
Adrian Laborde ◽  
Bernard Ducommun ◽  
Valérie Lobjois ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Aspect Ratio ◽  
High Aspect Ratio ◽  
Force Sensors ◽  
Tumour Spheroid ◽  
Multicellular Tumour Spheroid

A PDMS microdevice made of a circle of high aspect ratio micropillars, acting as flexible force sensors, dedicated to the investigation of mechanical properties on the tissue scale.

scholarly journals Modelling of tumour growth and cytotoxic effect of docetaxel in xenografts

2014 ◽  
Vol 114 (3) ◽  
pp. e3-e13 ◽  
Author(s):  
Neil D. Evans ◽  
Richard J. Dimelow ◽  
James W.T. Yates
Keyword(s):  
Cytotoxic Effect ◽  
Tumour Growth

Modelling of tumour growth and cytotoxic effect of taxotere in xenografts

2012 ◽  
Vol 45 (18) ◽  
pp. 278-283
Author(s):  
Neil D. Evans ◽  
Richard Dimelow ◽  
James W.T. Yates
Keyword(s):  
Cytotoxic Effect ◽  
Tumour Growth

Is there any similarity between tumour growth and thermal expansion?

2011 ◽  
Vol 226 (1) ◽  
pp. 192-201 ◽  
Author(s):  
A A Golneshan ◽  
H Nemati
Keyword(s):  
Thermal Expansion ◽  
Soft Tissue ◽  
Solid Tumour ◽  
Tumour Growth ◽  
Coefficient Of Thermal Expansion ◽  
Mathematical Proof ◽  
The Other ◽  
Balance Equations ◽  
Tumour Spheroid ◽  
Independent Variable

To model growth in a soft tissue, the tissue is considered as an open system wherein mass is not conserved. This leads to modifying the basic balance equations to accommodate this mass change. On the other hand, a number of researchers have assumed that there exists an analogy between tumour growth and thermal expansion phenomena. However, no mathematical proof yet has supported this analogy. In this paper, based on a new explanation of growth, the set of modified basic balance equations is closed using a new constitutive equation in conjunction with the Clausius–Duhem inequality to model the residual stresses in a growing solid tumour. Applying the model to two popular tumour types (carcinoma and tumour spheroid), which can be examined experimentally, revealed a perfect similarity between the resulting stresses in the growing tumour and those predicted by thermal expansion analogy, although the predicted displacements are different in these two models. Moreover, it is shown that the coefficient of growth expansion, analogous to the coefficient of thermal expansion, is not an independent variable and is related to the tissue measurable known quantities: Poisson’s ratio and density.

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