Strand displacement activated peroxidase activity of hemin for fluorescent DNA sensing

The Analyst ◽  
2015 ◽  
Vol 140 (19) ◽  
pp. 6532-6537 ◽  
Author(s):  
Quanbo Wang ◽  
Nan Xu ◽  
Zhen Gui ◽  
Jianping Lei ◽  
Huangxian Ju ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Peroxidase Activity ◽  
High Specificity ◽  
Dna Sensing ◽  
Strand Displacement ◽  
Peroxidase Mimic ◽  
Dna Strand Displacement ◽  
Dna Strand

The toehold-mediated DNA strand displacement was utilized to regulate the catalytic activity of the peroxidase mimic hemin and design a fluorescent DNA sensing method with high specificity.

scholarly journals Highly specific SNP detection using 2D graphene electronics and DNA strand displacement

2016 ◽  
Vol 113 (26) ◽  
pp. 7088-7093 ◽  
Author(s):  
Michael T. Hwang ◽  
Preston B. Landon ◽  
Joon Lee ◽  
Duyoung Choi ◽  
Alexander H. Mo ◽  
...  
Keyword(s):  
Personalized Medicine ◽  
High Specificity ◽  
Practical Implementation ◽  
Strand Displacement ◽  
Snp Detection ◽  
Single Nucleotide ◽  
Specificity And Sensitivity ◽  
Dna Strand Displacement ◽  
Wide Range ◽  
Dna Strand

Single-nucleotide polymorphisms (SNPs) in a gene sequence are markers for a variety of human diseases. Detection of SNPs with high specificity and sensitivity is essential for effective practical implementation of personalized medicine. Current DNA sequencing, including SNP detection, primarily uses enzyme-based methods or fluorophore-labeled assays that are time-consuming, need laboratory-scale settings, and are expensive. Previously reported electrical charge-based SNP detectors have insufficient specificity and accuracy, limiting their effectiveness. Here, we demonstrate the use of a DNA strand displacement-based probe on a graphene field effect transistor (FET) for high-specificity, single-nucleotide mismatch detection. The single mismatch was detected by measuring strand displacement-induced resistance (and hence current) change and Dirac point shift in a graphene FET. SNP detection in large double-helix DNA strands (e.g., 47 nt) minimize false-positive results. Our electrical sensor-based SNP detection technology, without labeling and without apparent cross-hybridization artifacts, would allow fast, sensitive, and portable SNP detection with single-nucleotide resolution. The technology will have a wide range of applications in digital and implantable biosensors and high-throughput DNA genotyping, with transformative implications for personalized medicine.

scholarly journals Kinetics of DNA Strand Displacement Systems with Locked Nucleic Acids

2017 ◽  
Vol 121 (12) ◽  
pp. 2594-2602 ◽  
Author(s):  
Xiaoping Olson ◽  
Shohei Kotani ◽  
Bernard Yurke ◽  
Elton Graugnard ◽  
William L. Hughes
Keyword(s):  
Nucleic Acids ◽  
Strand Displacement ◽  
Dna Strand Displacement ◽  
Locked Nucleic Acids ◽  
Dna Strand ◽  
Kinetics Of

Biocomputing based on DNA strand displacement reactions

ChemPhysChem ◽  
2021 ◽  
Author(s):  
Hui Lv ◽  
Qian Li ◽  
Jiye Shi ◽  
Fei Wang ◽  
Chunhai Fan
Keyword(s):  
Strand Displacement ◽  
Dna Strand Displacement ◽  
Displacement Reactions ◽  
Dna Strand

scholarly journals Kinetics of Toehold-Mediated DNA Strand Displacement Depend on FeII4L4 Tetrahedron Concentration

Nano Letters ◽  
2021 ◽  
Vol 21 (3) ◽  
pp. 1368-1374
Author(s):  
Jinbo Zhu ◽  
Filip Bošković ◽  
Bao-Nguyen T. Nguyen ◽  
Jonathan R. Nitschke ◽  
Ulrich F. Keyser
Keyword(s):  
Strand Displacement ◽  
Dna Strand Displacement ◽  
Dna Strand ◽  
Kinetics Of

Highly sensitive multiplex detection of microRNA by competitive DNA strand displacement fluorescence assay

Talanta ◽  
2019 ◽  
Vol 200 ◽  
pp. 487-493 ◽  
Author(s):  
Raja Chinnappan ◽  
Rawa Mohammed ◽  
Ahmed Yaqinuddin ◽  
Khalid Abu-Salah ◽  
Mohammed Zourob
Keyword(s):  
Fluorescence Assay ◽  
Multiplex Detection ◽  
Strand Displacement ◽  
Highly Sensitive ◽  
Dna Strand Displacement ◽  
Dna Strand

Development of DNA computing and information processing based on DNA-strand displacement

2015 ◽  
Vol 58 (10) ◽  
pp. 1515-1523 ◽  
Author(s):  
Yafei Dong ◽  
Chen Dong ◽  
Fei Wan ◽  
Jing Yang ◽  
Cheng Zhang
Keyword(s):  
Information Processing ◽  
Dna Computing ◽  
Strand Displacement ◽  
Dna Strand Displacement ◽  
Dna Strand
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