Comparing equilibrium and kinetic protein unfolding using time-resolved electrospray-coupled ion mobility mass spectrometry

The Analyst ◽  
2015 ◽  
Vol 140 (20) ◽  
pp. 6973-6979 ◽  
Author(s):  
Peter Liuni ◽  
Bin Deng ◽  
Derek J. Wilson
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Protein Unfolding ◽  
Ion Mobility Mass Spectrometry ◽  
Time Resolved

We apply a new hyphenated method, TRESI-IMS-MS, to compare equilibrium and kinetic unfolding intermediates of cytochrome c.

Time Resolved Native Ion-Mobility Mass Spectrometry to Monitor Dynamics of IgG4 Fab Arm Exchange and “Bispecific” Monoclonal Antibody Formation

2013 ◽  
Vol 85 (20) ◽  
pp. 9785-9792 ◽  
Author(s):  
François Debaene ◽  
Elsa Wagner-Rousset ◽  
Olivier Colas ◽  
Daniel Ayoub ◽  
Nathalie Corvaïa ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Monoclonal Antibody ◽  
Ion Mobility ◽  
Antibody Formation ◽  
Ion Mobility Mass Spectrometry ◽  
Time Resolved ◽  
Bispecific Monoclonal Antibody

Reliable Tracking In-Solution Protein Unfolding via Ultrafast Thermal Unfolding/Ion Mobility-Mass Spectrometry

2018 ◽  
Vol 90 (13) ◽  
pp. 7997-8001 ◽  
Author(s):  
Gongyu Li ◽  
Shihui Zheng ◽  
Yuting Chen ◽  
Zhuanghao Hou ◽  
Guangming Huang
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Protein Unfolding ◽  
Thermal Unfolding ◽  
Ion Mobility Mass Spectrometry

scholarly journals Ion mobility-mass spectrometry shows stepwise protein unfolding under alkaline conditions

2021 ◽  
Author(s):  
Cagla Sahin ◽  
Nicklas Österlund ◽  
Axel Leppert ◽  
Janne Johansson ◽  
Erik Marklund ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Protein Unfolding ◽  
Protein Denaturation ◽  
Native Mass Spectrometry ◽  
Ion Mobility Mass Spectrometry ◽  
Alkaline Conditions

Although native mass spectrometry is widely applied to monitor chemical or thermal protein denaturation, it is not clear to what extent it can inform about alkali-induced unfolding. Here, we probe...

Rapid Diagnosis of Parkinson’s Disease from Sebum using Paper Spray Ionisation Ion Mobility Mass Spectrometry

2020 ◽  
Author(s):  
Depanjan Sarkar ◽  
Drupad Trivedi ◽  
Eleanor Sinclair ◽  
Sze Hway Lim ◽  
Caitlin Walton-Doyle ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Ion Mobility ◽  
Neurodegenerative Disorder ◽  
Treatment Options ◽  
Ion Mobility Mass Spectrometry ◽  
Paper Spray ◽  
Molecular Features ◽  
Validation Set

Parkinson’s disease (PD) is the second most common neurodegenerative disorder for which identification of robust biomarkers to complement clinical PD diagnosis would accelerate treatment options and help to stratify disease progression. Here we demonstrate the use of paper spray ionisation coupled with ion mobility mass spectrometry (PSI IM-MS) to determine diagnostic molecular features of PD in sebum. PSI IM-MS was performed directly from skin swabs, collected from 34 people with PD and 30 matched control subjects as a training set and a further 91 samples from 5 different collection sites as a validation set. PSI IM-MS elucidates ~ 4200 features from each individual and we report two classes of lipids (namely phosphatidylcholine and cardiolipin) that differ significantly in the sebum of people with PD. Putative metabolite annotations are obtained using tandem mass spectrometry experiments combined with accurate mass measurements. Sample preparation and PSI IM-MS analysis and diagnosis can be performed ~5 minutes per sample offering a new route to for rapid and inexpensive confirmatory diagnosis of this disease.

P‐97: Separation of Isomeric OLED Materials Using Ion Mobility‐Mass Spectrometry (IM‐MS)

2021 ◽  
Vol 52 (1) ◽  
pp. 1444-1447
Author(s):  
Hirotaka Shioji ◽  
Azusa Uematsu ◽  
Motoshi Onoda ◽  
Keiko Matsuda ◽  
Keisuke Sawada ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Ion Mobility Mass Spectrometry

scholarly journals Ion Mobility–Mass Spectrometry for Bioanalysis

Separations ◽  
2021 ◽  
Vol 8 (3) ◽  
pp. 33
Author(s):  
Xavier Garcia ◽  
Maria del Mar Sabaté ◽  
Jorge Aubets ◽  
Josep Maria Jansat ◽  
Sonia Sentellas
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
Biological Samples ◽  
Ion Mobility Mass Spectrometry ◽  
Exogenous Compounds ◽  
Analytical Separation

This paper aims to cover the main strategies based on ion mobility spectrometry (IMS) for the analysis of biological samples. The determination of endogenous and exogenous compounds in such samples is important for the understanding of the health status of individuals. For this reason, the development of new approaches that can be complementary to the ones already established (mainly based on liquid chromatography coupled to mass spectrometry) is welcomed. In this regard, ion mobility spectrometry has appeared in the analytical scenario as a powerful technique for the separation and characterization of compounds based on their mobility. IMS has been used in several areas taking advantage of its orthogonality with other analytical separation techniques, such as liquid chromatography, gas chromatography, capillary electrophoresis, or supercritical fluid chromatography. Bioanalysis is not one of the areas where IMS has been more extensively applied. However, over the last years, the interest in using this approach for the analysis of biological samples has clearly increased. This paper introduces the reader to the principles controlling the separation in IMS and reviews recent applications using this technique in the field of bioanalysis.

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