Selective enrichment of metal-binding proteins based on magnetic core/shell microspheres functionalized with metal cations

The Analyst ◽  
2015 ◽  
Vol 140 (12) ◽  
pp. 4197-4205 ◽  
Author(s):  
Caiyun Fang ◽  
Lei Zhang ◽  
Xiaoqin Zhang ◽  
Haojie Lu
Keyword(s):  
Metal Ions ◽  
Metal Binding ◽  
Binding Proteins ◽  
Metal Cations ◽  
Magnetic Core ◽  
New Method ◽  
Magnetic Microspheres ◽  
Core Shell ◽  
Selective Enrichment

A new method based on magnetic microspheres with metal ions was developed and applied to characterize putative metal binding proteins.

scholarly journals Isolation of humic acid from oxidized lignite and complexation with metal cations

2017 ◽  
Vol 71 (4) ◽  
pp. 319-327
Author(s):  
Benjamin Catovic ◽  
Minela Sisic ◽  
Majda Srabovic ◽  
Melita Huremovic
Keyword(s):  
Humic Acid ◽  
Metal Ions ◽  
Humic Acids ◽  
Metal Binding ◽  
Base Material ◽  
Metal Cations ◽  
Biological Properties ◽  
Metal Nitrate ◽  
Chemical Difference ◽  
The Difference

Lignite is brown coal, which in its composition contains humic acids. Humic acids are produced by coal combustion, which leads to the enrichment of coal humic acids. Lignite, from the opet pit mine Sikulje, lignite ore ?Kreka?, Bosnia and Herzegovina, was fragmented and sieved to the appropriate size and used as a base material. The isolation of humic acid was carried out from pre-oxidized and dried lignite after which it was refined. Identification thus obtained humic acids was carried out by FTIR spectroscopy and its characterization of UV analysis which is determined by optical density of isolated humic acid and its complexation with metal cations. Data obtained by FTIR spectroscopic analysis of isolated humic acids show no significant structural and chemical difference in relation to the spectrum obtained for standard humic acids (Sigma Aldrich). UV analysis showed that isolated and standard humic acid have E4/E6 ratio in an appropriate range of 3?5, which indicates the presence of a large number of aliphatic structure. Based on the degree of humification was found that the isolated humic acids belong to the type B standard while humic acids belong to type A. The most important property of the humic substances is the ability to interact with the metal ions forming soluble or insoluble complexes which possess different chemical and biological properties and stability. The nature of the complex between humic acid and metal cation derived from the heterogeneous, polyelectric and polydispersive character humic acids that occurs due to the presence of a large number of functional groups. Complexation of humic acid is carried out with different concentrations of metal nitrate solutions and at different pH values. Different amounts of humic acids were used for the complexation. The amount of the free metal ions was measured with the ICP-OES methode. The data were also statistically analyzed with ANOVA. The results showed that increasing the pH reduces the concentration of metal ions adsorbed on humic acid and by increasing the concentrations and amounts of metal humic acid that power increases. On the basis of the difference in absorbance between metals and humic acids can be said that there is an interaction between the metal and the ligand and is based on absorbance values obtained can be determine the next set of metal binding to humic acids Pb>Zn>Ni>Cu.

scholarly journals mebipred: identifying metal-binding potential in protein sequences.

2021 ◽  
Author(s):  
Ariel A Aptekmann ◽  
Joy Buongiorno ◽  
Donato Giovannelli ◽  
Mihaela Glamoclija ◽  
Diego U Ferreiro ◽  
...  
Keyword(s):  
Metal Ions ◽  
Metal Binding ◽  
Binding Proteins ◽  
Rna Binding ◽  
Protein Structures ◽  
Ion Concentration ◽  
Binding Potential ◽  
Structure Stability ◽  
Computational Techniques ◽  
Sequence Alignments

Metal-binding proteins have a central role in maintaining life processes. Nearly one-third of known protein structures contain metal ions that are used for a variety of needs, such as catalysis, DNA/RNA binding, protein structure stability, etc. Identifying metal-binding proteins is thus crucial for understanding the mechanisms of cellular activity. However, experimental annotation of protein metal-binding potential is severely lacking, while computational techniques are often imprecise and of limited applicability. We developed a novel machine learning-based method, mebipred, for identifying metal-binding proteins from sequence-derived features. This method is nearly 90% accurate in recognizing proteins that bind metal ions and ion containing ligands. Moreover, the identity of ten ubiquitously present metal ions and ion-containing ligands can be annotated. mebipred is reference-free, i.e. no sequence alignments are involved, and outperforms other prediction methods, both in speed and accuracy. mebipred can also identify protein metal-binding capabilities from short sequence stretches and, thus, may be useful for the annotation of metagenomic samples metal requirements inferred from translated sequencing reads. We performed an analysis of microbiome data and found that ocean, hot spring sediments and soil microbiomes use a more diverse set of metals than human host-related ones. For human-hosted microbiomes, physiological conditions explain the observed metal preferences. Similarly, subtle changes in ocean sample ion concentration affect the abundance of relevant metal-binding proteins. These results are highlight mebipreds utility in analyzing microbiome metal requirements. mebipred is available as a web server at services.bromberglab.org/mebipred and as a standalone package at https://pypi.org/project/mymetal/

Oil Column Method for Magnetic Microspheres with Core-Shell Structure

2013 ◽  
Vol 743-744 ◽  
pp. 677-680
Author(s):  
Lian Qing Yu ◽  
Kai Tuo Dong ◽  
Qian Qian Zhi ◽  
Ming Jiang Cai
Keyword(s):  
Surface Area ◽  
Shell Structure ◽  
Magnetic Core ◽  
Magnetic Microspheres ◽  
Core Shell ◽  
Average Pore ◽  
Physical Strength ◽  
Catalyst Carrier ◽  
Shaping Time ◽  
Core Shell Structure

Spherical alumina catalysts have been widely used in fluidized bed and slurry-bed or catalyst carrier due to their excellent pore structure, large surface area, good physical strength and resistance to acids and stability. Magnetic microspheres of γ-Fe2O3 @ Al2O3 with core-shell structure were obtained by γ-Fe2O3 magnetic core coating with aluminum sol. After aging, drying and calcinations process, microspheres with certain hardness and magnetic properties can be obtained. During the preparation of core-shell structure microspheres, effect of both the content of aluminum sol, hexamethylenetetramine(HMT), magnetic core γ-Fe2O3 and process parameters of shaping time, aging temperature, calcination temperature on the properties of the spherical carrier was investigated. The optimum properties of microsphere carrier for hardness of 36.32 N/mm, specific surface area of 89.016 m2/g, pore volume of 0.874 cm3/g and average pore diameter 18.51 nm were synthesized, respectively.

Maltodextrin-Modified Magnetic Microspheres for Selective Enrichment of Maltose Binding Proteins

2014 ◽  
Vol 6 (5) ◽  
pp. 3568-3574 ◽  
Author(s):  
Jin Zheng ◽  
Chongjun Ma ◽  
Yangfei Sun ◽  
Miaorong Pan ◽  
Li Li ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Magnetic Microspheres ◽  
Selective Enrichment
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