A highly sensitive homogeneous electrochemical assay for alkaline phosphatase activity based on single molecular beacon-initiated T7 exonuclease-mediated signal amplification

The Analyst ◽  
2015 ◽  
Vol 140 (12) ◽  
pp. 4030-4036 ◽  
Author(s):  
Lianfang Zhang ◽  
Ting Hou ◽  
Haiyin Li ◽  
Feng Li
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Signal Amplification ◽  
Molecular Beacon ◽  
Electrochemical Assay ◽  
Highly Sensitive ◽  
T7 Exonuclease

A highly sensitive homogeneous electrochemical assay for alkaline phosphatase activity based on single molecular beacon-initiated T7 exonuclease-assisted signal amplification.

Highly sensitive amperometric measurement of alkaline phosphatase activity with glucose oxidase amplification

1995 ◽  
Vol 382 (1-2) ◽  
pp. 129-135 ◽  
Author(s):  
Leopoldo Della Ciana ◽  
Giuliana Bernacca ◽  
Franco Bordin ◽  
Simona Fenu ◽  
Fulvio Garetto
Keyword(s):  
Alkaline Phosphatase ◽  
Glucose Oxidase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Highly Sensitive

Membrane and nucleus targeting for highly sensitive cancer cell detection using pyrophosphate and alkaline phosphatase activity-mediated fluorescence switching of functionalized carbon dots

2018 ◽  
Vol 6 (37) ◽  
pp. 5992-6001 ◽  
Author(s):  
Cheong A Choi ◽  
Zihnil Adha Islamy Mazrad ◽  
Ji Hyun Ryu ◽  
Insik In ◽  
Kang Dae Lee ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Tumor Cells ◽  
Alkaline Phosphatase Activity ◽  
Carbon Dots ◽  
Cell Detection ◽  
Highly Sensitive ◽  
Fluorescence Switching ◽  
Cancer Cell Detection ◽  
Specific Membrane

Fluorescence-switching of Cu2+–CD for specific membrane and nucleus targeting based on PPi and ALP activity in tumor cells.

A sensitive dual colorimetric and fluorescence system for assaying the activity of alkaline phosphatase that relies on pyrophosphate inhibition of the peroxidase activity of copper ions

The Analyst ◽  
2014 ◽  
Vol 139 (18) ◽  
pp. 4691-4695 ◽  
Author(s):  
Ki Soo Park ◽  
Chang Yeol Lee ◽  
Hyun Gyu Park
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Peroxidase Activity ◽  
Copper Ions ◽  
Accurate Determination ◽  
Highly Sensitive ◽  
Optical Assay

A novel and highly sensitive optical assay for the accurate determination of alkaline phosphatase activity is developed by utilizing the peroxidase activity of Cu2+ ions.

LEUCOCYTE METABOLISM IN PREGNANCY

1960 ◽  
Vol XXXV (IV) ◽  
pp. 575-584 ◽  
Author(s):  
C. Borel ◽  
J. Frei ◽  
A. Vannotti
Keyword(s):  
Alkaline Phosphatase ◽  
Oxygen Consumption ◽  
Pregnant Women ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Lactate Production ◽  
Glucose Consumption ◽  
In Pregnancy

ABSTRACT Enzymatic studies, on leucocytes of pregnant women, show an increase of the alkaline phosphatase activity and a decrease of the glucose consumption and lactate production, as well as of proteolysis. The oxygen consumption, with succinate as substrate, does not vary.

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