An integrated closed-tube 2-plex PCR amplification and hybridization assay with switchable lanthanide luminescence based spatial detection

The Analyst ◽  
2015 ◽  
Vol 140 (12) ◽  
pp. 3960-3968 ◽  
Author(s):  
Susanne Lahdenperä ◽  
Anni Spangar ◽  
Anna-Maija Lempainen ◽  
Laura Joki ◽  
Tero Soukka
Keyword(s):  
Nucleic Acid ◽  
Pcr Amplification ◽  
Hybridization Assay ◽  
Lanthanide Luminescence ◽  
Closed Tube ◽  
Nucleic Acid Assay ◽  
Spatial Detection ◽  
Proof Of Principle

A proof-of-principle of a genuine closed-tube nucleic acid assay with integrated 2-plex PCR amplification and array-based detection has been presented.

Multifunctional Printable Micropattern Array for Digital Nucleic Acid Assay for Microbial Pathogen Detection

2021 ◽  
Vol 13 (2) ◽  
pp. 3098-3108
Author(s):  
Yunho Choi ◽  
Younseong Song ◽  
Yong Tae Kim ◽  
Seok Jae Lee ◽  
Kyoung G. Lee ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Pathogen Detection ◽  
Microbial Pathogen ◽  
Nucleic Acid Assay

Ultrasensitive Nucleic Acid Assay Based on Cyclometalated Iridium(III) Complex with High Electrochemiluminescence Efficiency

2020 ◽  
Author(s):  
Zhi-Hong Xu ◽  
Hang Gao ◽  
Nan Zhang ◽  
Wei Zhao ◽  
Yi-Xiang Cheng ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Assay

scholarly journals Screening Metagenomic Data for Viruses Using the E-Probe Diagnostic Nucleic Acid Assay

2014 ◽  
Vol 104 (10) ◽  
pp. 1125-1129 ◽  
Author(s):  
A. H. Stobbe ◽  
W. L. Schneider ◽  
P. R. Hoyt ◽  
U. Melcher
Keyword(s):  
Nucleic Acid ◽  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Metagenomic Data ◽  
Data Sets ◽  
Virus Species ◽  
Data Set ◽  
Golden Yellow ◽  
Nucleic Acid Assay ◽  
Ngs Data

Next generation sequencing (NGS) is not used commonly in diagnostics, in part due to the large amount of time and computational power needed to identify the taxonomic origin of each sequence in a NGS data set. By using the unassembled NGS data sets as the target for searches, pathogen-specific sequences, termed e-probes, could be used as queries to enable detection of specific viruses or organisms in plant sample metagenomes. This method, designated e-probe diagnostic nucleic acid assay, first tested with mock sequence databases, was tested with NGS data sets generated from plants infected with a DNA (Bean golden yellow mosaic virus, BGYMV) or an RNA (Plum pox virus, PPV) virus. In addition, the ability to detect and differentiate among strains of a single virus species, PPV, was examined by using probe sets that were specific to strains. The use of probe sets for multiple viruses determined that one sample was dually infected with BGYMV and Bean golden mosaic virus.

A novel high throughput nucleic acid sandwich hybridization assay on a gold substrate

2013 ◽  
Vol 5 (11) ◽  
pp. 2835 ◽  
Author(s):  
C. H. van den Kieboom ◽  
T. S. Y. van Domburg ◽  
M. I. de Jonge ◽  
G. Ferwerda ◽  
P. W. M. Hermans
Keyword(s):  
Nucleic Acid ◽  
High Throughput ◽  
Hybridization Assay ◽  
Gold Substrate ◽  
Sandwich Hybridization ◽  
Sandwich Hybridization Assay

scholarly journals Plasmon-enhanced fluorescence imaging with silicon-based silver chips for protein and nucleic acid assay

2017 ◽  
Vol 955 ◽  
pp. 98-107 ◽  
Author(s):  
Bing Yuan ◽  
Xiangxu Jiang ◽  
Chu Yao ◽  
Meimei Bao ◽  
Jiaojiao Liu ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Fluorescence Imaging ◽  
Enhanced Fluorescence ◽  
Plasmon Enhanced Fluorescence ◽  
Nucleic Acid Assay ◽  
Silicon Based

A Rapid and Accurate Detection Approach for Multidrug-Resistant Tuberculosis Based on PCR-ELISA Microplate Hybridization Assay

2020 ◽  
Vol 51 (6) ◽  
pp. 606-613
Author(s):  
Ye-Cheng Zhou ◽  
Shu-Mei He ◽  
Zi-Lu Wen ◽  
Jun-Wei Zhao ◽  
Yan-Zheng Song ◽  
...  
Keyword(s):  
Clinical Care ◽  
Pcr Amplification ◽  
Drug Susceptibility ◽  
Multidrug Resistant ◽  
Drug Susceptibility Testing ◽  
Hybridization Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Resistant Tuberculosis ◽  
Multidrug Resistant Tuberculosis ◽  
Mdr Tb

Abstract Rapid and accurate diagnosis of multidrug-resistant tuberculosis (MDR-TB) is important for timely and appropriate therapy. In this study, a rapid and easy-to-perform molecular test that integrated polymerase chain reaction (PCR) amplification and a specific 96-well microplate hybridization assay, called PCR-ELISA (enzyme-linked immunosorbent assay), were developed for detection of mutations in rpoB, katG, and inhA genes responsible for rifampin (RIF) and isoniazid (INH) resistance and prediction of drug susceptibility in Mycobacterium tuberculosis clinical isolates. We evaluated the utility of this method by using 32 multidrug-resistent (MDR) isolates and 22 susceptible isolates; subsequently, we compared the results with data obtained by conventional drug susceptibility testing and DNA sequencing. The sensitivity and specificity of the PCR-ELISA test were 93.7% and 100% for detecting RIF resistance, and 87.5% and 100% for detecting INH resistance, respectively. These results were comparable to those yielded by commercially available molecular tests such as the GenoType MTBDRplus assay. Based on the aforementioned results, we conclude that the PCR-ELISA microplate hybridization assay is a rapid, inexpensive, convenient, and reliable test that will be useful for rapid diagnosis of MDR-TB, for improved clinical care.

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