scholarly journals Toxicity of arsenite and thio-DMAV after long-term (21 days) incubation of human urothelial cells: cytotoxicity, genotoxicity and epigenetics

2014 ◽  
Vol 3 (6) ◽  
pp. 456-464 ◽  
Author(s):  
Marlies Unterberg ◽  
Larissa Leffers ◽  
Florian Hübner ◽  
Hans-Ulrich Humpf ◽  
Konstantin Lepikhov ◽  
...  
Keyword(s):  
Arsenic Species ◽  
Urothelial Cells ◽  
Modes Of Action

This study aims to further mechanistically understand toxic modes of action after chronic arsenic species exposure.

scholarly journals Associations between Arsenic Species in Exfoliated Urothelial Cells and Prevalence of Diabetes among Residents of Chihuahua, Mexico

2014 ◽  
Vol 122 (10) ◽  
pp. 1088-1094 ◽  
Author(s):  
Jenna M. Currier ◽  
María C. Ishida ◽  
Carmen González-Horta ◽  
Blanca Sánchez-Ramírez ◽  
Lourdes Ballinas-Casarrubias ◽  
...  
Keyword(s):  
Arsenic Species ◽  
Urothelial Cells

Bioresorbable Microspheres as Injectable Cell Carrier: FRom Preparation to in Vitro Evaluation

1998 ◽  
Vol 550 ◽  
Author(s):  
Y. Senuma ◽  
S. Franceschin ◽  
J. G. Hilborn ◽  
P. Tissiéres ◽  
P. Frey
Keyword(s):  
Cultured Cells ◽  
Urothelial Cells ◽  
New Approach ◽  
Muscular Structure ◽  
Cell Carrier ◽  
Support Matrix ◽  
Vesico Ureteral Reflux

AbstractA new approach to the vesico-ureteral reflux could be a local regeneration of the defective vesicoureteral junction by transplanting living cells to the target site. The aim of this work is to provide a long-term effective treatment by producing bioresorbable microspheres which can act as support matrix for those cells, with the goal of an in vivo transfer of the in vitro cultured cells with a minimal surgical procedure. After microsphere degradation, the cells should be integrated into the muscular structure of the junction. Most innovative is that these are cultured muscle and urothelial cells from the bladder of the same patient.

Anti-cancer palladium complexes: a focus on PdX2L2, palladacycles and related complexes

2014 ◽  
Vol 43 (13) ◽  
pp. 4751-4777 ◽  
Author(s):  
Anant R. Kapdi ◽  
Ian J. S. Fairlamb
Keyword(s):  
Chemotherapeutic Agents ◽  
Palladium Complexes ◽  
Modes Of Action ◽  
Anti Cancer

Much success has been achieved with platinum-based chemotherapeutic agents,i.e.through interactions with DNA. The long-term application of Pt complexes is thwarted by issues, leading scientists to examine other metals such as palladium which could exhibit complementary modes of action.

EXPANSION AND LONG-TERM CULTURE OF DIFFERENTIATED NORMAL RAT UROTHELIAL CELLS IN VITRO

2001 ◽  
Vol 37 (7) ◽  
pp. 419 ◽  
Author(s):  
YUAN YUAN ZHANG ◽  
BARBARA LUDWIKOWSKI ◽  
ROBERT HURST ◽  
PETER FREY
Keyword(s):  
Urothelial Cells ◽  
Long Term Culture ◽  
Normal Rat

176 Long-term culture of urothelial cells: Effects on gene expression and proliferation

2014 ◽  
Vol 13 (1) ◽  
pp. e176
Author(s):  
C.I. Chamorro ◽  
S. Zeiai ◽  
Engberg G. Reinfeldt ◽  
A. Nordenskjöld ◽  
M. Fossum ◽  
...  
Keyword(s):  
Gene Expression ◽  
Urothelial Cells ◽  
Long Term Culture

scholarly journals Measuring thousands of single vesicle leakage events reveals the mode of action of antimicrobial peptides

2021 ◽  
Author(s):  
Kareem Al Nahas ◽  
Marcus Fletcher ◽  
Katharine Hammond ◽  
Christian Nehls ◽  
Jehangir Cama ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Host Defense ◽  
Mode Of Action ◽  
Antimicrobial Agents ◽  
Spatiotemporal Resolution ◽  
Detailed Understanding ◽  
Modes Of Action ◽  
Structure Activity ◽  
Single Vesicle

Host defense or antimicrobial peptides hold promise for providing new pipelines of effective antimicrobial agents. Their activity quantified against model phospholipid membranes is fundamental to a detailed understanding of their structure-activity relationships. However, existing characterization assays lack the resolution necessary to achieve this insight. Leveraging a highly parallelized microfluidic platform for trapping and studying thousands of giant unilamellar vesicles, we conducted quantitative long-term microscopy studies to monitor the membrane-disruptive activity of archetypal antimicrobial peptides with a high spatiotemporal resolution. We described the modes of action of these peptides via measurements of the disruption of the vesicle population under the conditions of continuous peptide dosing using a range of concentrations, and related the observed modes with the molecular activity mechanisms of these peptides. The study offers an effective approach for characterizing membrane-targeting antimicrobial agents in a standardized manner, and for assigning specific modes of action to the corresponding antimicrobial mechanisms.

scholarly journals Long-Term Toxicological Monitoring of a Multibarrier Advanced Wastewater Treatment Plant Comprising Ozonation and Granular Activated Carbon with In Vitro Bioassays

Water ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 3245
Author(s):  
Lam T. Phan ◽  
Heidemarie Schaar ◽  
Daniela Reif ◽  
Sascha Weilguni ◽  
Ernis Saracevic ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Activated Carbon ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Granular Activated Carbon ◽  
Advanced Treatment ◽  
Modes Of Action ◽  
In Vitro Bioassays

A set of CALUX in vitro bioassays was applied for long-term toxicity monitoring at an advanced wastewater treatment plant comprising ozonation and granular activated carbon filtration for the abatement of contaminants of emerging concern (CEC). During the 13-month monitoring, eight reporter gene assays targeting different modes of action along the cellular toxicity pathway were accessed to evaluate the suitability and robustness of the technologies. Two approaches were followed: on the one hand, signal reduction during advanced treatment was monitored; on the other hand, results were compared to currently available effect-based trigger values (EBTs). A decrease of the corresponding biological equivalent concentrations after the multibarrier system could be observed for all modes of action; while the estrogenic activity decreased below the EBT already during ozonation, the potencies of oxidative stress-like and toxic PAH-like compounds still exceeded the discussed EBT after advanced treatment. Overall, the long-term monitoring confirmed the positive effect of the multibarrier system, commonly evaluated only by CEC abatement based on chemical analysis. It could be demonstrated that advanced WWTPs designed for CEC abatement are suitable to significantly decrease toxicity responses not only in the frame of pilot studies but under real-world conditions as well.

scholarly journals Arsenic species and selected metals in human urine: validation of HPLC/ICPMS and ICPMS procedures for a long-term population-based epidemiological study

2012 ◽  
Vol 4 (2) ◽  
pp. 406 ◽  
Author(s):  
Jürgen Scheer ◽  
Silvia Findenig ◽  
Walter Goessler ◽  
Kevin A. Francesconi ◽  
Barbara Howard ◽  
...  
Keyword(s):  
Epidemiological Study ◽  
Human Urine ◽  
Arsenic Species ◽  
Population Based

scholarly journals Extended performance analysis of polyurethane–iron oxide nanocomposite for efficient removal of arsenic species from water

2016 ◽  
Vol 17 (3) ◽  
pp. 889-896 ◽  
Author(s):  
Faten B. Hussein ◽  
Nidal H. Abu-Zahra
Keyword(s):  
Iron Oxide ◽  
Operation Mode ◽  
Absorption Spectrometry ◽  
Arsenic Species ◽  
Removal Capacity ◽  
Pu Foam ◽  
Cyclic Operation ◽  
Removal Of Arsenic ◽  
20 Nm

A polyurethane (PU) foam nanocomposite impregnated with iron oxide nanoparticles (IONPs) was developed to remove arsenic (As) from drinking water at ppb concentrations. The effect of synthesis and application parameters such as the size of IONPs, pH levels, weight of adsorbents, and arsenic concentrations on the performance of PU-IONP adsorbents in removing arsenic were studied. The prepared adsorbents were characterized by scanning electron microscopy and energy dispersive X-ray microscopy to evaluate the microstructure of PU-IONPs and the surface adsorption of arsenic species, respectively. Atomic absorption spectrometry was conducted to measure the concentration of arsenic in the treated solutions in order to calculate the removal capacity of PU-IONPs. The experimental results revealed that decreasing the size of IONPs from 50–100 nm to 15–20 nm yields a higher removal capacity. Increasing the weight of the used adsorbents and the contact time led to an increase in the removal capacity as well. As the arsenic species (III and V) concentration increased in the solution, the removal capacity of PU-IONPs decreased. In a column study, a long-term cyclic operation mode was found to be very effective in removing arsenic; 100% removal capacity was achieved when 500 ml of As solution (100 ppb) was treated.

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