Additive nanocomplexes of cationic lipopolymers for improved non-viral gene delivery to mesenchymal stem cells

2015 ◽  
Vol 3 (19) ◽  
pp. 3972-3982 ◽  
Author(s):  
Remant Bahadur KC ◽  
Cezary Kucharski ◽  
Hasan Uludağ
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Hyaluronic Acid ◽  
Gene Delivery ◽  
Viral Gene ◽  
Viral Gene Delivery

Additive polyplexes composed of cationic lipopolymers and hyaluronic acid–pDNA combination for implementing gene delivery to mesenchymal stem cells.

scholarly journals Genetic modification of mesenchymal stem cells to enhance their anti-tumor efficacy

2021 ◽  
pp. 1-6
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gene Delivery ◽  
Genetic Modification ◽  
Chemokine Receptors ◽  
Viral Vectors ◽  
Viral Gene ◽  
Delivery Methods ◽  
Differentiation Potential ◽  
Viral Gene Delivery

Non-hematopoietic mesenchymal stem cells (MSCs) are widely used in regenerative medicine and tissue engineering as they possess multilineage differentiation potential and self-renewal properties. MSCs can be easily isolated from several tissues and expanded following standard cell culture procedures. MSCs have the capability of mobilization to the tumor site; so, they can automatically relocate to the tumor sites through their chemokine receptors following intravenous transplantation. In this respect, they can be used for MSC-based gene therapy. In this therapeutic technique, beneficial genes are inserted by viral and non-viral methods into MSCs that lead to transgene expression in them. Genetic modifications of MSCs have been widely studied and thoroughly investigated to further enhance their therapeutic efficacy. The current strategies of MSC-based therapies emphasize the incorporation of beneficial genes, which will enhance the therapeutic ability of MSCs and have better homing efficiency. Non-viral methods produce less toxicity and immunogenicity compared to viral gene delivery methods and thus represent a promising and efficient tool for the genetic engineering of MSCs. Several non-viral gene delivery strategies have been developed in recent decades, and some of them have been used for MSCs modification. This mini review provides an overview of current gene delivery approaches used for the genetic modification of MSCs with beneficial genes including viral and non-viral vectors.

Non-Viral Gene Delivery to Mesenchymal Stem Cells: Methods, Strategies and Application in Bone Tissue Engineering and Regeneration

2011 ◽  
Vol 11 (1) ◽  
pp. 46-57 ◽  
Author(s):  
Jose L. Santos ◽  
Deepti Pandita ◽  
Joao Rodrigues ◽  
Ana P. Pego ◽  
Pedro L. Granja ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gene Delivery ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Viral Gene ◽  
Viral Gene Delivery

scholarly journals Genetic engineering of mesenchymal stem cells by non-viral gene delivery

2014 ◽  
Vol 58 (1) ◽  
pp. 19-48 ◽  
Author(s):  
Weiwei Wang ◽  
Xun Xu ◽  
Zhengdong Li ◽  
Andreas Lendlein ◽  
Nan Ma
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gene Delivery ◽  
Genetic Engineering ◽  
Viral Gene ◽  
Viral Gene Delivery

Minicircle microporation-based non-viral gene delivery improved the targeting of mesenchymal stem cells to an injury site

Biomaterials ◽  
2016 ◽  
Vol 101 ◽  
pp. 310-320 ◽  
Author(s):  
Ji-Young Mun ◽  
Keun Koo Shin ◽  
Ohsuk Kwon ◽  
Yong Taik Lim ◽  
Doo-Byoung Oh
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gene Delivery ◽  
Viral Gene ◽  
Injury Site ◽  
Viral Gene Delivery

Non-viral gene delivery of DNA polyplexed with nanoparticles transfected into human mesenchymal stem cells

Biomaterials ◽  
2010 ◽  
Vol 31 (1) ◽  
pp. 124-132 ◽  
Author(s):  
Ji S. Park ◽  
Kun Na ◽  
Dae G. Woo ◽  
Han N. Yang ◽  
Jung M. Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gene Delivery ◽  
Human Mesenchymal Stem Cells ◽  
Viral Gene ◽  
Viral Gene Delivery

scholarly journals Growth Factor-free Chondrogenic Differentiation from Induced Pluripotent Stem Cells using Minicircle Vectors

2018 ◽  
Author(s):  
Yeri Alice Rim ◽  
Yoojun Nam ◽  
Ji Hyeon Ju
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Growth Factor ◽  
Gene Delivery ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Bone Morphogenetic Protein 2 ◽  
Viral Gene ◽  
Induced Pluripotent ◽  
Viral Gene Delivery

The human degenerative cartilage has low regenerative potential. Chondrocyte transplantation offers a promising strategy for cartilage treatment and regeneration. Currently chondrogenesis using human pluripotent stem cells are accomplished using human recombinant growth factors. Here, we differentiated human induced pluripotent stem cells (hiPSCs) into chondrocytes and cartilage pellet using minicircle vectors. Minicircles are used as a non-viral gene delivery system for gene therapy in various diseases. Non-viral gene delivery can produce growth factors without integrating into the host genome. Minicircle vectors containing bone morphogenetic protein 2 (BMP2) and transforming growth factor, beta 3 (TGFβ3) were successfully generated and delivered to hiPSC-derived outgrowth (OG) cells. Cell pellets generated using minicircle-transfected OG cells successfully differentiated into chondrogenic lineage. Chondrogenic pellets transfected with growth factor-encoding minicircles effectively recovered osteochondral defect in rat models. Taken together, this work shows the potential application of minicircles in cartilage regeneration using hiPSCs.

scholarly journals Non-viral gene delivery of HIF-1α promotes angiogenesis in human adipose-derived stem cells

2020 ◽  
Vol 113 ◽  
pp. 279-288
Author(s):  
Savannah E. Est-Witte ◽  
Ashley L. Farris ◽  
Stephany Y. Tzeng ◽  
Daphne L. Hutton ◽  
Dennis H. Gong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Delivery ◽  
Viral Gene ◽  
Adipose Derived Stem Cells ◽  
Viral Gene Delivery
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