Light-responsible DNA hydrogel–gold nanoparticle assembly for synergistic cancer therapy

2015 ◽  
Vol 3 (8) ◽  
pp. 1537-1543 ◽  
Author(s):  
Jaejung Song ◽  
Sekyu Hwang ◽  
Kyuhyun Im ◽  
Jaehyun Hur ◽  
Jutaek Nam ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cancer Therapy ◽  
Gold Nanoparticle ◽  
Nanoparticle Assembly ◽  
Delivery Vehicle ◽  
Combination Cancer Therapy ◽  
Synergistic Combination ◽  
Dna Hydrogel

A DNA hydrogel based delivery vehicle that combines assembled gold nanoparticles and Doxorubicins showed light-triggered and synergistic combination cancer therapy at the in vitro and in vivo levels.

Silencing Bag-1 gene via magnetic gold nanoparticle-delivered siRNA plasmid for colorectal cancer therapy in vivo and in vitro

Tumor Biology ◽  
2016 ◽  
Vol 37 (8) ◽  
pp. 10365-10374 ◽  
Author(s):  
Wenbai Huang ◽  
Zhan’ao Liu ◽  
Guanzhou Zhou ◽  
Jianmin Ling ◽  
Ailing Tian ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Therapy ◽  
Gold Nanoparticle

Augmented tumor accumulation and photothermal ablation using gold nanoparticles with a particular cellular entry orientation

2018 ◽  
Vol 33 (6) ◽  
pp. 660-675
Author(s):  
Gwang Jin Noh ◽  
Hongsuk Park ◽  
Eun Seong Lee
Keyword(s):  
Gold Nanoparticles ◽  
Tumor Cell ◽  
Gold Nanoparticle ◽  
Cellular Internalization ◽  
Tat Peptide ◽  
Peptide Expression ◽  
Cellular Entry ◽  
Tumor Accumulation

Gold nanoparticles with various functionalities have served as potential tools in nanotechnology for tumor ablation. In this work, we seek to design and develop gold nanoparticle with poly(ethylene glycol)-containing dopamine (hereafter termed as AuND), and to synthesize the AuND with one-sided Tat peptide expression (OT@AuND). We demonstrate the tumor cell-targeting ability on the basis of anti-nonspecific cell binding of OT@AuND and determine how the chemically modified gold nanoparticle–based product affects photothermal tumor therapy in vitro and in vivo. The OT@AuND with a particular cellular entry orientation–induced delayed endocytosis, which is advantageous for enhanced permeability and retention effect-based tumor accumulation. This is because the slower cellular interaction of OT@AuND allows it to have the time to be transported to and bind to the tumor site. In tumor cell lines, OT@AuND showed a lower cellular uptake than gold nanoparticles with full-sided Tat peptide expression (FT@AuND) in the early period (after its in vitro and in vivo administration), but the cellular internalization rate of OT@AuND caught up with that of FT@AuND in the late period. Importantly, the delayed cellular internalization feature of OT@AuND resulted in efficient tumor accumulation in tumor-bearing mice, because the time interval provided OT@AuND more chances not to bind to any cells, but to enter tumor cells, leading to selective photothermal tumor ablation. These data suggest that gold nanoparticles with a particular cellular entry orientation can be further explored as a potential photothermal therapeutic agent and as a strategy to treat tumors.

Two-dimensional transition metal dichalcogenide nanomaterials for combination cancer therapy

2017 ◽  
Vol 5 (10) ◽  
pp. 1873-1895 ◽  
Author(s):  
Linji Gong ◽  
Liang Yan ◽  
Ruyi Zhou ◽  
Jiani Xie ◽  
Wei Wu ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Transition Metal ◽  
Combination Therapy ◽  
Transition Metal Dichalcogenide ◽  
Two Dimensional ◽  
Combination Cancer Therapy

In this review, we mainly summarize the latest advances in the utilization of 2D TMDCs for PTT combination cancer therapy and imaging-guided cancer combination therapy, as well as their toxicity bothin vitroandin vivo.

Self-Monitoring and Self-Delivery of Photosensitizer-Doped Nanoparticles for Highly Effective Combination Cancer Therapy in Vitro and in Vivo

ACS Nano ◽  
2015 ◽  
Vol 9 (10) ◽  
pp. 9741-9756 ◽  
Author(s):  
Jinfeng Zhang ◽  
Yu-Chuan Liang ◽  
Xudong Lin ◽  
Xiaoyue Zhu ◽  
Li Yan ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Self Monitoring ◽  
Effective Combination ◽  
Combination Cancer Therapy ◽  
Highly Effective

Radioactive Gold Nanoparticle in Two Forms (19879Au GNPs and 99mTc-GNPs) for Lung Cancer Antiproliferative Induction and Intralesional Imaging: A Proof of Concept

2020 ◽  
Vol 20 (14) ◽  
pp. 1648-1653 ◽  
Author(s):  
Hongwei Xu ◽  
Shengpan Jiang ◽  
Jimin Wang ◽  
Xuebing Li ◽  
Tingwei Wu ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Au Nanoparticles ◽  
A549 Cells ◽  
Intralesional Injection ◽  
Proof Of Concept ◽  
Efficient Treatment

Background: Lung cancer is among the most common cancers worldwide, responsible for 13% of all new cancer cases. Also, it is the leading cause of cancer death among both men and women. In this scenario, an effective and efficient treatment is required. Objective: Production of two gold nanoparticles: 198Au and 99mTc-Au. The first one has been produced from irradiation of the 197Au in order to produce a beta-emitter gold nanoparticle for cancer therapy. The second one has been produced from the radiolabeling of gold nanoparticles with technetium 99 metastable in order to produce imaging nanoagent. Methods: The 198Au nanoparticles were produced by irradiation and identified by hyper-purity germanium (HPGe). They were then evaluated in vitro in order to confirm the behavior on cell proliferation of lung cancer cell lines by the MTT methodology using A549 cells. The 99mTc-Au nanoparticles were produced by directradiolabeling with 99mTc and evaluated in vivo as intralesional nanoagent. Results: The results showed that in both cases, all the nanoparticles have performed their duties with excellence. The 198Au nanoparticles were capable to kill lung cancer cells, while 99mTc-Au was capable to image the tumor after intralesional injection. In addition, 99mTc-Au nanoparticles were useful for biodistribution assay imaging, showing the main organs responsible for the nanoparticle uptake in healthy animals. Conclusion: Both gold nanoparticles showed to be a highly efficient nanoagent for both: therapy and diagnosing of lung cancer.

Dosimetry and Radioenhancement Comparison of Gold Nanoparticles in Kilovoltage and Megavoltage Radiotherapy using MAGAT Polymer Gel Dosimeter

2019 ◽  
Vol 9 (2Apr) ◽  
Author(s):  
S Farahani ◽  
N Riyahi Alam ◽  
S Haghgoo ◽  
M Khoobi ◽  
Gh Geraily ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
3D Visualization ◽  
Energy Levels ◽  
Polymer Gel ◽  
External Radiotherapy ◽  
Dose Enhancement ◽  
Internal Radiotherapy ◽  
Boost Radiotherapy

Background: Numerous unique characteristics of the nanosized gold, including high atomic number, low toxicity, and high biocompatibility make it one of the most appropriate nanostructures to boost radiotherapy efficacy. Many in-vivo and in-vitro investigations have indicated that gold nanoparticles (AuNPs) can significantly increase tumor injuries in low kilovoltage radiotherapy. While deep-lying tumors require much higher energy levels with greater penetration power, and investigations carried out in megavoltage energy range show contradictory results.Objective: In this study, we quantitatively assess and compare dose enhancement factors (DEFs) obtained through AuNPs under radiation of Cobalt-60 source (1.25MeV) versus Iridium-192 source (0.380 KeV) using MAGAT gel dosimeter.Material and Methods: MAGAT polymer gel in both pure and combined with 0.2 mM AuNPs was synthesized. In order to quantify the effect of energy on DEF, irradiation was carried out by Co-60 external radiotherapy and Ir-192 internal radiotherapy. Finally, readings of irradiated and non-irradiated gels were performed by MR imaging.Result: The radiation-induced R2 (1/T2) changes of the gel tubes doped with AuNPs compared to control samples, upon irradiation of beams released by Ir-192 source showed a significant dose enhancement (15.31% ±0.30) relative to the Co-60 external radiotherapy (5.85% ±0.14).Conclusion: This preliminary study suggests the feasibility of using AuNPs in radiation therapy (RT), especially in low-energy sources of brachytherapy. In addition, MAGAT polymer gel, as a powerful dosimeter, could be used for 3D visualization of radiation dose distribution of AuNPs in radiotherapy.

Interference of Gold Nanoparticles with In vitro Endotoxin Detection Assays

2020 ◽  
Vol 16 (2) ◽  
pp. 204-213 ◽  
Author(s):  
Melissa A. Vetten ◽  
Mary Gulumian
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Surface Reactivity ◽  
In Vitro Assays ◽  
Limulus Amebocyte Lysate ◽  
Chromogenic Assay ◽  
Nanoparticle Suspensions ◽  
Endotoxin Contamination ◽  
Factor C

Background: Endotoxin-free engineered nanoparticle suspensions are imperative for their successful applications in the field of nanomedicine as well as in the investigations in their toxicity. Gold nanoparticles are known to interfere with various in vitro assays due to their optical properties and potential for surface reactivity. In vitro endotoxin testing assays are known to be susceptible to interference caused by the sample being tested. Objective: This study aimed to identify a preferred assay for the testing of endotoxin contamination in gold nanoparticle suspensions. Methods: The interference by gold nanoparticles on three assays namely, the commonly used limulus amebocyte lysate chromogenic assay, the limulus amebocyte lysate gel-clot method, and the less common recombinant Factor C (rFC) assay, was tested. Results: Possible interference could be observed with all three assays. The interference with the absorbance- based chromogenic assay could not be overcome by dilution; whilst the qualitative nature of the gel-clot assay excluded the possibility of distinguishing between a false positive result due to enhancement of the sensitivity of the assay, and genuine endotoxin contamination. However, interference with the rFC assay was easily overcome through dilution. Conclusion: The rFC assay is recommended as an option for endotoxin contamination detection in gold nanoparticle suspensions.

scholarly journals Radiosensitization of Prostate Cancers In Vitro and In Vivo to Erbium-filtered Orthovoltage X-rays Using Actively Targeted Gold Nanoparticles

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Allison M. Khoo ◽  
Sang Hyun Cho ◽  
Francisco J. Reynoso ◽  
Maureen Aliru ◽  
Kathryn Aziz ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
X Rays ◽  
Prostate Cancers

Biocompatibility of gold nanoparticles: In-vitro and In-vivo study

2021 ◽  
Vol 42 ◽  
pp. 3041-3045
Author(s):  
Rua J. Kadhim ◽  
Esraa H. Karsh ◽  
Zainab J. Taqi ◽  
Majid S. Jabir
Keyword(s):  
Gold Nanoparticles ◽  
In Vivo Study

scholarly journals In vitro and in vivo applications of Euphorbia wallichii shoot extract-mediated gold nanospheres

2021 ◽  
Vol 10 (1) ◽  
pp. 101-111
Author(s):  
Rehman Ullah ◽  
Sumaira Shah ◽  
Zahir Muhammad ◽  
Sajjad Ali Shah ◽  
Shah Faisal ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Muscular Contraction ◽  
Surface Plasmon Resonance Peak ◽  
Plasmon Resonance Peak ◽  
Optical Absorption Peak ◽  
Visible Spectra ◽  
Scattering Spectra ◽  
Dose Dependent

Abstract The current study was designed to investigate the potential of Euphorbia wallichii shoot extract for reducting Au3+ and stabilizing gold nanoparticles. UV-visible spectra of gold nanoparticles showed obvious surface plasmon resonance peak at 548 nm. Microscopy (SEM and TEM) showed spherical dimensions, and the energy dispersive X-ray spectra displayed the strongest optical absorption peak for gold (Au) at 2.1 keV. Dynamic light scattering spectra represent polydispersed mixture with particulate diameter of 2.5–103.2 nm. The IR spectra confirm the potential functional groups of shoot extract responsible for the reduction of Au3+ to gold nanoparticles which exhibit tremendous antibacterial potential of 76.31%, 68.47%, 79.85%, 48.10%, and 65.53% against Escherichia coli, Staphylococcus aureus, Bacillus pumilus, Pseudomonas aeruginosa, and Klebsiella pneumoniae, respectively. Gold nanoparticles showed markedly elevated fungicidal potency compared to the shoot extract alone against the tested fungal strains. IC50 for 2,2-diphenyl-1-picrylhydrazyl scavenging was 31.52, 18.29, and 15.32 µg/mL at 30, 60, and 90 min of reaction time, respectively. Both shoot extract and nanoparticles revealed 71% mortality at 100 µg/mL, with LD90 values of 310.56 µg/mL. Experimental mice acquired dose-dependent analgesia of 54.21%, 82.60%, and 86.53% when treated with gold nanoparticles at 50, 100, and 200 mg/kg bw. Inhibition of gastrointestinal muscular contraction was 21.16%, 30.49%, and 40.19% in mice feed with 50, 100, and 200 mg/kg bw, respectively.

Sign in / Sign up

Export Citation Format

Share Document