Fluorination on polyethylenimine allows efficient 2D and 3D cell culture gene delivery

2015 ◽  
Vol 3 (4) ◽  
pp. 642-650 ◽  
Author(s):  
Jia Lv ◽  
Hong Chang ◽  
Yu Wang ◽  
Mingming Wang ◽  
Jianru Xiao ◽  
...  
Keyword(s):  
Cell Culture ◽  
Gene Delivery ◽  
Cell Cultures ◽  
Gene Transfection ◽  
3D Cell Culture ◽  
Transfection Efficacy ◽  
3D Cell Cultures ◽  
2D And 3D

Fluorinationviaanhydride and oxirane reactions enhances the gene transfection efficacy of PEI on 3D cell cultures.

Ratiometric Nanoviscometers: Applications for Measuring Cellular Physical Properties in 3D Cultures

2020 ◽  
Vol 25 (3) ◽  
pp. 234-246
Author(s):  
Charles McRae White ◽  
Mark A. Haidekker ◽  
William S. Kisaalita
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Cell Biology ◽  
Low Cost ◽  
3D Cell Culture ◽  
Biomechanical Properties ◽  
Practical Applications ◽  
3D Cell Cultures

New insights into the biomechanical properties of cells are revealing the importance of these properties and how they relate to underlying molecular, architectural, and behavioral changes associated with cell state and disease processes. However, the current understanding of how these in vitro biomechanical properties are associated with in vivo processes has been developed based on the traditional monolayer (two-dimensional [2D]) cell culture, which traditionally has not translated well to the three-dimensional (3D) cell culture and in vivo function. Many gold standard methods and tools used to observe the biomechanical properties of 2D cell cultures cannot be used with 3D cell cultures. Fluorescent molecules can respond to external factors almost instantaneously and require relatively low-cost instrumentation. In this review, we provide the background on fluorescent molecular rotors, which are attractive tools due to the relationship of their emission quantum yield with environmental microviscosity. We make the case for their use in both 2D and 3D cell cultures and speculate on their fundamental and practical applications in cell biology.

scholarly journals Comparison of antioxidant activity between cyanidin-3-O-glucoside (C3G) liposome and cyanidin-3-O-glucoside (C3G) in 2D and 3D cell cultures

2018 ◽  
Author(s):  
Tisong Liang ◽  
Rongfa Guan ◽  
Guozhou Cao ◽  
Haitao Shen ◽  
Zhenfeng Liu ◽  
...  
Keyword(s):  
Cell Culture ◽  
Antioxidant Activity ◽  
Cell Cultures ◽  
Cell Model ◽  
3D Cell Culture ◽  
Culture Model ◽  
Mda Content ◽  
2D And 3D ◽  
Cells Cultured

ABSTRACTThe 2D cell culture is the predominant in vitro model for numerous studies. However, 2D cell cultures may not accurately reflect the functions of three-dimensional (3D) tissues, which have extensive cell–cell and cell–matrix interactions; thus, using 2D cell cultures may lead to inaccurate experimental results. Therefore, to obtain adequate and detailed information about the antioxidant activity of cyanidin-3-O-glucoside (C3G) and C3G liposomes in the 2D and 3D cell culture models, we used in this study H2O2to construct the cell damage model and assess the antioxidant activity of C3G and C3G liposomes on Caco-2 cells cultured in the 3D model. We also measured the cell viability, cell morphology, and activity of glutathione (GSH), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), and malondialdehyde (MDA) content of Caco-2 cells treated with H2O2, C3G, and C3G liposomes. Results showed that cells cultured in the 3D culture model formed a 3D structure and tight spheroids and showed increased cell activity and IC50. The C3G and C3G liposomes can enhance the activity of GSH, SOD, and T-AOC but decrease the MDA content. At the same time, the effect was more obvious in the 3D cell culture model than in the cells cultured in the 2D model. This study revealed that the results obtained from the 2D cell model may be inaccurate compared with the results obtained from the 3D cell model. A realistic mechanism study of antioxidant activity of C3G and C3G liposomes in the 3D cell model, which acts as an intermediate stage bridging the in vitro 2D and in vivo models, was observed.

scholarly journals Upscaling of pneumatic membrane valves for the integration of 3D cell cultures on chip

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Nina Compera ◽  
Scott Atwell ◽  
Johannes Wirth ◽  
Bernhard Wolfrum ◽  
Matthias Meier
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Large Scale ◽  
3D Cell Culture ◽  
3D Cell Cultures ◽  
Culture Formation ◽  
Large Scale Integration ◽  
3D Printed ◽  
On Chip ◽  
Scale Integration

For integration of 3D cell cultures on microfluidic large-scale integration chips, we upscaled pneumatic membrane valves using 3D-printed replica molds. Unit cell operations for 3D cell culture formation, culture, retrieval, and fusion are designed.

scholarly journals Simplified One-stop Protocols to Extract Collagen from Various Animal Tissues for 3D Cell Cultures

2020 ◽  
Author(s):  
John A. Terrell ◽  
Chengpeng Chen
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
3D Cell Culture ◽  
Animal Tissues ◽  
3D Cell Cultures ◽  
One Stop

In this manuscript, we present a one-stop protocol to extract collagen from various animal tissues, and apply it to fabricate 3D cell culture scaffolds.

scholarly journals Simplified One-stop Protocols to Extract Collagen from Various Animal Tissues for 3D Cell Cultures

2020 ◽  
Author(s):  
John A. Terrell ◽  
Chengpeng Chen
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
3D Cell Culture ◽  
Animal Tissues ◽  
3D Cell Cultures ◽  
One Stop

In this manuscript, we present a one-stop protocol to extract collagen from various animal tissues, and apply it to fabricate 3D cell culture scaffolds.

scholarly journals SpheroidPicker: An Automated 3D cell culture manipulator robot using deep learning

2020 ◽  
Author(s):  
Istvan Grexa ◽  
Akos Diosdi ◽  
Andras Kriston ◽  
Nikita Moshkov ◽  
Maria Harmati ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
High Throughput Screening ◽  
Ex Vivo ◽  
Low Cost ◽  
3D Cell Culture ◽  
Experimental Conditions ◽  
The Past ◽  
3D Cell Cultures ◽  
Manipulator Robot

AbstractRecent statistics report that more than 3.7 million new cases of cancer occur in Europe yearly, and the disease accounts for approximately 20 % of all deaths. High-throughput screening of cancer cell cultures has dominated the search for novel, effective anticancer therapies in the past decades. Recently, ex vivo 3D cell cultures from the patient’s own cancer cells have gained importance. We recently evaluated the major advancements and needs of the 3D cell cultures screening field, and we concluded that strictly standardized sample preparation is the most desired development. Here we propose an artificial intelligence-guided low-cost 3D cell culture delivery system. It consists of a light microscope, a micromanipulator, a syringe pump, and a controller computer. The system performs morphology-based feature analysis on spheroids and transfers the most appropriate ones between various sample holders. It can select the samples from standard sample holders, including Petri dishes and microwell plates, and then transfer them to a variety of holders up to 384 well plates. The device performs reliable semi- and fully automated spheroid transfer. This results in highly controlled experimental conditions and eliminates non-trivial side effects of sample variability that is a key aspect towards next-generation precision medicine.

An antioxidant self-healing hydrogel for 3D cell cultures

2020 ◽  
Vol 8 (7) ◽  
pp. 1383-1388 ◽  
Author(s):  
Lei Yang ◽  
Yuan Zeng ◽  
Haibo Wu ◽  
Chunwu Zhou ◽  
Lei Tao
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Biginelli Reaction ◽  
3D Cell Culture ◽  
Self Healing ◽  
3D Cell Cultures

An antioxidant self-healing hydrogel has been facilely prepared using the Biginelli reaction and then used for 3D cell culture.

scholarly journals Correction: Assessment of the toxicity and inflammatory effects of different-sized zinc oxide nanoparticles in 2D and 3D cell cultures

RSC Advances ◽  
2020 ◽  
Vol 10 (72) ◽  
pp. 44397-44397
Author(s):  
Zhipan Wu ◽  
Rongfa Guan ◽  
Miao Tao ◽  
Fei Lyu ◽  
Guozhou Cao ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Cell Cultures ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
3D Cell Cultures ◽  
2D And 3D

Correction for ‘Assessment of the toxicity and inflammatory effects of different-sized zinc oxide nanoparticles in 2D and 3D cell cultures’ by Zhipan Wu, Rongfa Guan, Miao Tao et al., RSC Adv., 2017, 7, 12437–12445, DOI: 10.1039/C6RA27334C.

Layer-by-Layer Biomimetic Microgels for 3D Cell Culture and Nonviral Gene Delivery

2021 ◽  
Author(s):  
Bruna G. Carvalho ◽  
Franciele F. Vit ◽  
Hernandes F. Carvalho ◽  
Sang W. Han ◽  
Lucimara G. de la Torre
Keyword(s):  
Cell Culture ◽  
Gene Delivery ◽  
3D Cell Culture ◽  
Nonviral Gene Delivery ◽  
Layer By Layer

scholarly journals Conjunctival melanoma and electrochemotherapy: preliminary results using 2D and 3D cell culture models in vitro

2018 ◽  
Vol 97 (4) ◽  
pp. e632-e640 ◽  
Author(s):  
Miltiadis Fiorentzis ◽  
Periklis Katopodis ◽  
Helen Kalirai ◽  
Berthold Seitz ◽  
Arne Viestenz ◽  
...  
Keyword(s):  
Cell Culture ◽  
3D Cell Culture ◽  
Conjunctival Melanoma ◽  
Preliminary Results ◽  
Culture Models ◽  
2D And 3D ◽  
Cell Culture Models
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