In vitro antibacterial capacity and cytocompatibility of SiO2–CaO–P2O5 meso-macroporous glass scaffolds enriched with ZnO

2014 ◽  
Vol 2 (30) ◽  
pp. 4836-4847 ◽  
Author(s):  
Sandra Sánchez-Salcedo ◽  
Shruti Shruti ◽  
Antonio J. Salinas ◽  
Gianluca Malavasi ◽  
Ledi Menabue ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Cell Development ◽  
Osteoblast Cell ◽  
Macroporous Glass ◽  
Antibacterial Capacity

The biocompatibility and the antibacterial capability of meso-macroporous bioactive glass scaffolds in the SiO2–CaO–P2O5–ZnO system were investigated. Zn2+ ions release greatly influenced the osteoblast cell development and provides antibacterial capability against S. aureus.

scholarly journals Flexible organic–inorganic hybrid bioceramic for bone tissue regeneration

2020 ◽  
Vol 10 (04) ◽  
pp. 2050013
Author(s):  
Jing Chen ◽  
Xingmei Zhang ◽  
Beibei Li ◽  
Yawei Yang
Keyword(s):  
Simulated Body Fluid ◽  
Bioactive Glass ◽  
Tissue Regeneration ◽  
Body Fluid ◽  
Bone Tissue Regeneration ◽  
Osteoblast Cell ◽  
Butylene Succinate ◽  
Inorganic Hybrid ◽  
Cell Biocompatibility

Development of novel biomaterials for bone regeneration is based on the sufficient bone-bonding ability, bioactivity and biocompatibility. In this study, novel flexible poly(butylene succinate)/polydimethysiloxane-modified bioactive glass/nano-hydroxyapatite (PBSu/PDMS-BG/nHA) hybrid bioceramic with various nHA concentration on the in vitro bone-like hydroxyapatite (HA) formation, biomineralization activity and osteoblast cell biocompatibility were investigated. The rapid precipitation of HA on the hybrid bioceramic surfaces was found after being immersed in simulated body fluid (SBF) for seven days. Results show that the amount of HA deposition increased with the increase of nHA concentration. The optimized PBSu/PDMS-BG/nHA hybrid bioceramic exhibited good flexibility, high biomineralization activity and good osteoblast cell biocompatibility.

scholarly journals Bioactive glass S53P4 eradicates Staphylococcus aureus in biofilm/planktonic states in vitro

2020 ◽  
Vol 125 (3) ◽  
pp. 217-225
Author(s):  
Torstein Grønseth ◽  
Lene K. Vestby ◽  
Live L. Nesse ◽  
Magnus von Unge ◽  
Juha T. Silvola
Keyword(s):  
Staphylococcus Aureus ◽  
Bioactive Glass

scholarly journals Altered Erythrocytes and a Leaky Block in B-Cell Development in CD24/HSA-Deficient Mice

Blood ◽  
1997 ◽  
Vol 89 (3) ◽  
pp. 1058-1067 ◽  
Author(s):  
P.J. Nielsen ◽  
B. Lorenz ◽  
A.M. Müller ◽  
R.H. Wenger ◽  
F. Brombacher ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
B Cell ◽  
Embryonic Stem ◽  
Cell Development ◽  
B Cell Development ◽  
Surface Glycoprotein ◽  
Malarial Parasite ◽  
Heat Stable Antigen ◽  
Deficient Mice

Abstract The heat stable antigen (HSA, or murine CD24) is a glycosyl phosphatidylinositol-linked surface glycoprotein expressed on immature cells of most, if not all, major hematopoietic lineages, as well as in developing neural and epithelial cells. It has been widely used to stage the maturation of B and T lymphocytes because it is strongly induced and then repressed again during their maturation. Terminally differentiated lymphocytes, as well as most myeloid lineages, are negative for HSA. Erythrocytes are an exception in that they maintain high levels of HSA expression. HSA on naive B cells has been shown to mediate cell-cell adhesion, while HSA on antigen-presenting cells has been shown to mediate a costimulatory signal important for activating T lymphocytes during an immune response. Here, we characterize mice that lack a functional HSA gene, constructed by homologous recombination in embryonic stem cells. While T-cell and myeloid development appears normal, these mice show a leaky block in B-cell development with a reduction in late pre-B and immature B-cell populations in the bone marrow. Nevertheless, peripheral B-cell numbers are normal and no impairment of immune function could be detected in these mice in a variety of immunization and infection models. We also observed that erythrocytes are altered in HSA-deficient mice. They show a higher tendency to aggregate and are more susceptible to hypotonic lysis in vitro. In vivo, the mean half-life of HSA-deficient erythrocytes was reduced. When infected with the malarial parasite Plasmodium chabaudi chabaudi, the levels of parasite-bearing erythrocytes in HSA-deficient mice were also significantly elevated, but the mice were able to clear the infection with kinetics similar to wild-type mice and were immune to a second challenge. Thus, apart from alterations in erythrocytes and a mild block in B-cell development, the regulated expression of HSA appears to be dispensable for the maturation and functioning of those cell lineages that normally express it.

scholarly journals Synthesis and Characterization of Silver–Strontium (Ag-Sr)-Doped Mesoporous Bioactive Glass Nanoparticles

Gels ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 34
Author(s):  
Shaher Bano ◽  
Memoona Akhtar ◽  
Muhammad Yasir ◽  
Muhammad Salman Maqbool ◽  
Akbar Niaz ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Composite Coatings ◽  
Antibacterial Properties ◽  
Bone Diseases ◽  
Osteogenic Potential ◽  
Diffusion Method ◽  
Charge Composition ◽  
In Vitro Bioactivity ◽  
Mesoporous Bioactive Glass

Biomedical implants are the need of this era due to the increase in number of accidents and follow-up surgeries. Different types of bone diseases such as osteoarthritis, osteomalacia, bone cancer, etc., are increasing globally. Mesoporous bioactive glass nanoparticles (MBGNs) are used in biomedical devices due to their osteointegration and bioactive properties. In this study, silver (Ag)- and strontium (Sr)-doped mesoporous bioactive glass nanoparticles (Ag-Sr MBGNs) were prepared by a modified Stöber process. In this method, Ag+ and Sr2+ were co-substituted in pure MBGNs to harvest the antibacterial properties of Ag ions, as well as pro-osteogenic potential of Sr2 ions. The effect of the two-ion concentration on morphology, surface charge, composition, antibacterial ability, and in-vitro bioactivity was studied. Scanning electron microscopy (SEM), X-Ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) confirmed the doping of Sr and Ag in MBGNs. SEM and EDX analysis confirmed the spherical morphology and typical composition of MBGNs, respectively. The Ag-Sr MBGNs showed a strong antibacterial effect against Staphylococcus carnosus and Escherichia coli bacteria determined via turbidity and disc diffusion method. Moreover, the synthesized Ag-Sr MBGNs develop apatite-like crystals upon immersion in simulated body fluid (SBF), which suggested that the addition of Sr improved in vitro bioactivity. The Ag-Sr MBGNs synthesized in this study can be used for the preparation of scaffolds or as a filler material in the composite coatings for bone tissue engineering.

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