A one-step and scalable production route to metal nanocatalyst supported polymer nanospheres via flash nanoprecipitation

2014 ◽  
Vol 2 (41) ◽  
pp. 17286-17290 ◽  
Author(s):  
Rui Liu ◽  
Chris Sosa ◽  
Yao-Wen Yeh ◽  
Fengli Qu ◽  
Nan Yao ◽  
...  
Keyword(s):  
Flash Nanoprecipitation ◽  
Production Route ◽  
One Step ◽  
Polymer Nanospheres ◽  
Scalable Production

scholarly journals One-step synthesis of Ag@PS nanospheres via flash nanoprecipitation

2019 ◽  
Vol 33 (3) ◽  
pp. e4713 ◽  
Author(s):  
Xianxiang Hu ◽  
Zhijie Zhang ◽  
Yao Lu ◽  
Rui Liu ◽  
Lei Sun ◽  
...  
Keyword(s):  
Flash Nanoprecipitation ◽  
One Step

Ionic Flash NanoPrecipitation (iFNP) for the facile, one-step synthesis of inorganic–organic hybrid nanoparticles in water

Nanoscale ◽  
2017 ◽  
Vol 9 (4) ◽  
pp. 1403-1408 ◽  
Author(s):  
N. M. Pinkerton ◽  
L. Behar ◽  
K. Hadri ◽  
B. Amouroux ◽  
C. Mingotaud ◽  
...  
Keyword(s):  
Hybrid Nanoparticles ◽  
Flash Nanoprecipitation ◽  
Organic Hybrid ◽  
One Step

Efficient preparation of size tunable PEGylated gold nanoparticles

2016 ◽  
Vol 4 (28) ◽  
pp. 4813-4817 ◽  
Author(s):  
Christina Tang ◽  
Chris L. Sosa ◽  
Robert F. Pagels ◽  
Rodney D. Priestley ◽  
Robert K. Prud'homme
Keyword(s):  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Flash Nanoprecipitation ◽  
One Step ◽  
Translational Imaging

Flash NanoPrecipitation enables rapid, scalable, one-step synthesis of size-tunable PEGylated gold nanoparticles for drug delivery and translational imaging applications.

scholarly journals CRISPR-mediated multigene integration enables Shikimate pathway refactoring for enhanced 2-phenylethanol biosynthesis in Kluyveromyces marxianus

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Mengwan Li ◽  
Xuye Lang ◽  
Marcos Moran Cabrera ◽  
Sawyer De Keyser ◽  
Xiyan Sun ◽  
...  
Keyword(s):  
Kluyveromyces Marxianus ◽  
Shikimate Pathway ◽  
Pathway Engineering ◽  
Fuel Additive ◽  
Range Analysis ◽  
Production Route ◽  
Fragrance Compound ◽  
One Step ◽  
Selection For ◽  
The One

Abstract Background 2-phenylethanol (2-PE) is a rose-scented flavor and fragrance compound that is used in food, beverages, and personal care products. Compatibility with gasoline also makes it a potential biofuel or fuel additive. A biochemical process converting glucose or other fermentable sugars to 2-PE can potentially provide a more sustainable and economical production route than current methods that use chemical synthesis and/or isolation from plant material. Results We work toward this goal by engineering the Shikimate and Ehrlich pathways in the stress-tolerant yeast Kluyveromyces marxianus. First, we develop a multigene integration tool that uses CRISPR-Cas9 induced breaks on the genome as a selection for the one-step integration of an insert that encodes one, two, or three gene expression cassettes. Integration of a 5-kbp insert containing three overexpression cassettes successfully occurs with an efficiency of 51 ± 9% at the ABZ1 locus and was used to create a library of K. marxianus CBS 6556 strains with refactored Shikimate pathway genes. The 33-factorial library includes all combinations of KmARO4, KmARO7, and KmPHA2, each driven by three different promoters that span a wide expression range. Analysis of the refactored pathway library reveals that high expression of the tyrosine-deregulated KmARO4K221L and native KmPHA2, with the medium expression of feedback insensitive KmARO7G141S, results in the highest increase in 2-PE biosynthesis, producing 684 ± 73 mg/L. Ehrlich pathway engineering by overexpression of KmARO10 and disruption of KmEAT1 further increases 2-PE production to 766 ± 6 mg/L. The best strain achieves 1943 ± 63 mg/L 2-PE after 120 h fed-batch operation in shake flask cultures. Conclusions The CRISPR-mediated multigene integration system expands the genome-editing toolset for K. marxianus, a promising multi-stress tolerant host for the biosynthesis of 2-PE and other aromatic compounds derived from the Shikimate pathway.

Facile one step method realizing scalable production of g-C3N4nanosheets and study of their photocatalytic H2evolution activity

2014 ◽  
Vol 2 (44) ◽  
pp. 18924-18928 ◽  
Author(s):  
Xiuli Lu ◽  
Kun Xu ◽  
Pengzuo Chen ◽  
Kaicheng Jia ◽  
Si Liu ◽  
...  
Keyword(s):  
Step Method ◽  
One Step ◽  
Scalable Production

We report a facile one step method for fabricating g-C3N4nanosheets with enhanced photocatalytic H2evolution activity.

scholarly journals Growth of Reduced Graphene Oxide

2014 ◽  
Vol 1702 ◽  
Author(s):  
Jingfeng Huang ◽  
Hu Chen ◽  
Derrick Fam ◽  
Steve H. Faulkner ◽  
Wenbin Niu ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Electrical Resistance ◽  
Large Scale ◽  
Chemical Vapour ◽  
The Novel ◽  
Production Route ◽  
Reduced Graphene ◽  
Pressure Chemical ◽  
Scalable Production

ABSTRACTReduced graphene oxide (RGO) has the advantage of an aqueous and industrial-scalable production route. However, one of the main limitations that prevent the use of RGO in electronics is the high electrical resistance deviation between fabricated chips. In this article, we present the novel growth of RGO which can bridge the gaps in-between existing flakes and thus reduce the electrical resistance standard deviation from 80.5 % to 16.5 %. The average resistivity of the treated RGO of ∼ 3.8 nm thickness was 200 Ω/square. The study uses an atmospheric-pressure chemical vapour deposition (CVD) system with hydrogen and argon gas bubbling through ethanol before entering the furnace. With a treatment of 2 hours, 100 % of the silicon dioxide substrate was covered with RGO from an initial 65 % coverage. This technology could enable large-scale application of RGO use in practical electronic devices.

scholarly journals Purification and Characterization of Recombinant Expressed Apple Allergen Mal d 1

2020 ◽  
Vol 4 (1) ◽  
pp. 3
Author(s):  
Julia A. H. Kaeswurm ◽  
Bettina Nestl ◽  
Sven M. Richter ◽  
Max Emperle ◽  
Maria Buchweitz
Keyword(s):  
Metal Ion ◽  
Cd Spectroscopy ◽  
Anion Exchange Column ◽  
Production Route ◽  
Allergenic Potential ◽  
Sds Page ◽  
Thawing Process ◽  
Mal D 1 ◽  
One Step ◽  
The Impact

Mal d 1 is the primary apple allergen in northern Europe. To explain the differences in the allergenicity of apple varieties, it is essential to study its properties and interaction with other phytochemicals, which might modulate the allergenic potential. Therefore, an optimized production route followed by an unsophisticated purification step for Mal d 1 and respective mutants is desired to produce sufficient amounts. We describe a procedure for the transformation of the plasmid in competent E. coli cells, protein expression and rapid one-step purification. r-Mal d 1 with and without a polyhistidine-tag are purified by immobilized metal ion affinity chromatography (IMAC) and fast-protein liquid chromatography (FPLC) using a high-resolution anion-exchange column, respectively. Purity is estimated by SDS-PAGE using an image-processing program (Fiji). For both mutants an appropriate yield of r-Mal d 1 with purity higher than 85% is achieved. The allergen is characterized after tryptic in gel digestion by peptide analyses using HPLC-MS/MS. Secondary structure elements are calculated based on CD-spectroscopy and the negligible impact of the polyhistidine-tag on the folding is confirmed. The formation of dimers is proved by mass spectrometry and reduction by DTT prior to SDS-PAGE. Furthermore, the impact of the freeze and thawing process, freeze drying and storage on dimer formation is investigated.

Scalable Production of Therapeutic Protein Nanoparticles Using Flash Nanoprecipitation

2018 ◽  
Vol 8 (6) ◽  
pp. 1801010 ◽  
Author(s):  
Zhipeng Zeng ◽  
Cong Dong ◽  
Pengfei Zhao ◽  
Zhijia Liu ◽  
Lixin Liu ◽  
...  
Keyword(s):  
Therapeutic Protein ◽  
Flash Nanoprecipitation ◽  
Protein Nanoparticles ◽  
Scalable Production

A one-step approach to green and scalable production of graphene inks for printed flexible film heaters

2021 ◽  
Author(s):  
Lingyun Xu ◽  
Haopeng Wang ◽  
Yadong Wu ◽  
Zian Wang ◽  
Lixin Wu ◽  
...  
Keyword(s):  
Flexible Film ◽  
One Step ◽  
Scalable Production

Green and scalable production of graphene inks by microfluidization for preparing flexible film heaters.

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