scholarly journals DNA-based visual majority logic gate with one-vote veto function

2015 ◽  
Vol 6 (3) ◽  
pp. 1973-1978 ◽  
Author(s):  
Daoqing Fan ◽  
Kun Wang ◽  
Jinbo Zhu ◽  
Yong Xia ◽  
Yanchao Han ◽  
...  
Keyword(s):  
Logic Gate ◽  
Label Free ◽  
Majority Logic ◽  
First Time

A label-free and enzyme-free three-input majority logic gate with one-vote veto function was developed for the first time.

scholarly journals Recognition of Bimolecular Logic Operation Pattern Based on a Solid-State Nanopore

Sensors ◽  
2020 ◽  
Vol 21 (1) ◽  
pp. 33
Author(s):  
Han Yan ◽  
Zhen Zhang ◽  
Ting Weng ◽  
Libo Zhu ◽  
Pang Zhang ◽  
...  
Keyword(s):  
Self Assembly ◽  
Detection Method ◽  
Logic Gate ◽  
Detection Methods ◽  
Label Free ◽  
Recognition Method ◽  
Operation Pattern ◽  
Simple Detection ◽  
Dna Tetrahedron ◽  
Marker Molecule

Nanopores have a unique advantage for detecting biomolecules in a label-free fashion, such as DNA that can be synthesized into specific structures to perform computations. This method has been considered for the detection of diseased molecules. Here, we propose a novel marker molecule detection method based on DNA logic gate by deciphering a variable DNA tetrahedron structure using a nanopore. We designed two types of probes containing a tetrahedron and a single-strand DNA tail which paired with different parts of the target molecule. In the presence of the target, the two probes formed a double tetrahedron structure. As translocation of the single and the double tetrahedron structures under bias voltage produced different blockage signals, the events could be assigned into four different operations, i.e., (0, 0), (0, 1), (1, 0), (1, 1), according to the predefined structure by logic gate. The pattern signal produced by the AND operation is obviously different from the signal of the other three operations. This pattern recognition method has been differentiated from simple detection methods based on DNA self-assembly and nanopore technologies.

scholarly journals Nanoscale mapping of newly-synthesised phospholipid molecules in a biological cell using tip-enhanced Raman spectroscopy

2017 ◽  
Vol 53 (16) ◽  
pp. 2451-2454 ◽  
Author(s):  
Naresh Kumar ◽  
Marek M. Drozdz ◽  
Haibo Jiang ◽  
Daniela M. Santos ◽  
David J. Vaux
Keyword(s):  
Raman Spectroscopy ◽  
Biological Cell ◽  
Label Free ◽  
Tip Enhanced Raman Spectroscopy ◽  
First Time ◽  
Non Destructive

Non-destructive & label-free nanoscale mapping of newly-synthesised phospholipid molecules inside a biological cell is demonstrated using tip-enhanced Raman spectroscopy for the first time.

A Label-Free Fluorescent AND Logic Gate Aptasensor for Carbohydrate Antigen 15-3 Detection Based on Graphene Oxide

2021 ◽  
Vol 24 ◽  
Author(s):  
Wenxiao Hu ◽  
Yafei Dong ◽  
Luhui Wang ◽  
Yue Wang ◽  
Mengyao Qian ◽  
...  
Keyword(s):  
Fluorescent Dyes ◽  
Logic Gate ◽  
High Sensitivity ◽  
Carbohydrate Antigen ◽  
Fluorescence Signal ◽  
Label Free ◽  
Molecular Logic ◽  
Long Stem ◽  
Middle Ring ◽  
Fluorescent Aptasensor

Background: Molecular logic gate always used fluorescent dyes to realize fluorescence signal. The labeling of the fluorophore is relatively expensive, low yield and singly labeled impuritiesaffects the affinity between the target and the aptamer. Label-free fluorescent aptamer biosensor strategy has attracted widespread interest due to lower cost and simple. Objective: Herein, we have designed a AND logic gate fluorescent aptasensor for detecting carbohydrate antigen 15-3(CA15-3) based on label-free fluorescence signal output. Materials and Methods: A hairpin DNA probe consists of CA15-3 aptamer and partly anti-CA15-3 aptamer sequences as a long stem and G-rich sequences of the middle ring as a quadruplex-forming oligomer. G-rich sequences can fold into a quadruplex by K+, and then G-quadruplex interacts specifically with N-methylmesoporphyrin IX(NMM), leading to a dramatic increase in fluorescence of NMM. With CA15-3 and NMM as the two inputs, the fluorescence intensity of the NMM is the output signal. Lacking of CA15-3 or NMM, there is no significant fluorescence enhancing, and the output of the signal is “0”. The fluorescence signal was dramatically increasing and the output of the signal is “1” only when CA15-3 protein and NMM were added at the same time. Results: This biosensor strategy possessed selectivity, high sensitivity for detecting CA15-3 protein from 10 to 500 U mL-1 and the detection limit was 10 U mL-1, and also showed good reproducibility in spiked human serum. Conclusion: In summary, the proposed AND logic gate fluorescent aptasensor could specifically detect CA15-3.

Tracking the Formation and Degradation of Fatty-Acid-Accumulated Mitochondria Using Label-Free Chemical Imaging

2020 ◽  
Author(s):  
Chi Zhang ◽  
Stephen Boppart
Keyword(s):  
Fatty Acid ◽  
Structural Features ◽  
Chemical Imaging ◽  
Live Cells ◽  
Label Free ◽  
Dynamic Changes ◽  
Regulatory Processes ◽  
Transport Chain ◽  
Anti Stokes ◽  
First Time

Abstract The mitochondrion is one of the key organelles for maintaining cellular homeostasis. External environmental stimuli and internal regulatory processes alter the metabolism and functions of mitochondria. To understand these activities of mitochondria, it is critical to probe the key metabolic molecules inside these organelles. In this study, we used label-free chemical imaging modalities including coherent anti-Stokes Raman scattering and multiphoton-excited autofluorescence to study the mitochondrial activities in living cancer cells. We found that hypothermia exposure tends to induce fatty-acid (FA) accumulation in some mitochondria of MIAPaCa-2 cells. Autofluorescence images show that the FA-accumulated mitochondria also have abnormal NADH and FAD metabolism, likely induced by the dysfunction of the electron transport chain. We also found that when the cells were re-warmed to physiological temperature after a period of hypothermia, the FA-accumulated mitochondria changed their structural features, likely caused by the mitophagy process. To the best of our knowledge, this is the first time that FA accumulation in mitochondria was observed in live cells. Our research also demonstrates that multimodal label-free chemical imaging is an attractive tool to discover abnormal functions of mitochondria at the single-organelle level and can be used to quantify the dynamic changes of this organelle under perturbative conditions.

A highly sensitive and label-free electrochemiluminescence immunosensor for beta 2-microglobulin

2017 ◽  
Vol 9 (17) ◽  
pp. 2570-2577 ◽  
Author(s):  
Mohammad Rizwan ◽  
Noor Faizah Mohd-Naim ◽  
Natasha Ann Keasberry ◽  
Minhaz Uddin Ahmed
Keyword(s):  
Quantum Dot ◽  
Cadmium Selenide ◽  
Gold Nanoparticle ◽  
Label Free ◽  
Screen Printed Electrode ◽  
Highly Sensitive ◽  
Beta 2 ◽  
Beta 2 Microglobulin ◽  
First Time ◽  
Screen Printed

A highly sensitive and label-free electrochemiluminescence (ECL) immunosensor was fabricated for the detection of β-2-microglobulin (β2M) based on a gold nanoparticle-doped@carbon nano-onion chitosan nanocomposite modified cadmium selenide quantum dot screen-printed electrode (QDs-SPE/AuNPs@CNOs-CS) for the first time.

scholarly journals Simultaneous Detection of Dopamine and Serotonin—A Comparative Experimental and Theoretical Study of Neurotransmitter Interactions

Biosensors ◽  
2018 ◽  
Vol 9 (1) ◽  
pp. 3 ◽  
Author(s):  
Felicia Manciu ◽  
Marian Manciu ◽  
John Ciubuc ◽  
Emma Sundin ◽  
Katia Ochoa ◽  
...  
Keyword(s):  
Experimental Study ◽  
Theoretical Study ◽  
Simultaneous Detection ◽  
Pharmaceutical Research ◽  
Label Free ◽  
Neural Processes ◽  
Experimental Approaches ◽  
Biological Relationship ◽  
First Time ◽  
Accurate Quantification

With the goal of accurately detecting and quantifying the amounts of dopamine (DA) and serotonin (5-HT) in mixtures of these neurotransmitters without using any labelling, we present a detailed, comparative computational and Raman experimental study. Although discrimination between these two analytes is achievable in such mixtures for concentrations in the millimolar range, their accurate quantification remains unattainable. As shown for the first time in this work, the formation of a new composite resulting from their interactions with each other is the main reason for this lack of quantification. While this new hydrogen-bonded complex further complicates potential analyte discrimination and quantification at concentrations characteristic of physiological levels (i.e., nanomolar concentrations), it can also open new avenues for its use in drug delivery and pharmaceutical research. This remark is based not only on chemical interactions analyzed here from both theoretical and experimental approaches, but also on biological relationship, with consideration of both functional and neural proximity perspectives. Thus, this research constitutes an important contribution toward better understanding of neural processes, as well as toward possible future development of label-free biosensors.

Multiplexed determination of human growth hormone and prolactin at a label free electrochemical immunosensor using dual carbon nanotube–screen printed electrodes modified with gold and PEDOT nanoparticles

The Analyst ◽  
2014 ◽  
Vol 139 (18) ◽  
pp. 4556-4563 ◽  
Author(s):  
V. Serafín ◽  
G. Martínez-García ◽  
L. Agüí ◽  
P. Yáñez-Sedeño ◽  
J. M. Pingarrón
Keyword(s):  
Growth Hormone ◽  
Carbon Nanotube ◽  
Simultaneous Determination ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Electrochemical Immunosensor ◽  
Label Free ◽  
Screen Printed Electrodes ◽  
First Time

A label-free dual electrochemical immunosensor for simultaneous determination of human growth and prolactin hormones was prepared for the first time.

scholarly journals Proposal of Global Strain Clocking Scheme for Majority Logic Gate

IEEE Access ◽  
2020 ◽  
Vol 8 ◽  
pp. 77802-77810
Author(s):  
Yabo Chen ◽  
Xiaokuo Yang ◽  
Bo Wei ◽  
Huanqing Cui ◽  
Mingxu Song
Keyword(s):  
Logic Gate ◽  
Majority Logic ◽  
Global Strain

scholarly journals Majority Logic Decoding Under Data-Dependent Logic Gate Failures

2017 ◽  
Vol 63 (10) ◽  
pp. 6295-6306 ◽  
Author(s):  
Srdan Brkic ◽  
Predrag Ivanis ◽  
Bane Vasic
Keyword(s):  
Logic Gate ◽  
Majority Logic ◽  
Majority Logic Decoding
Sign in / Sign up

Export Citation Format

Share Document